Investigations on the binding of SYBR Green I to double-stranded (ds)DNA

Vitzthum, Frank; Zipper, Hubert; mmle, Katrin L; Buta, Christiane; Brunner, Herwig; Bernhagen, J.

doi:10.1240/sav_gbm_2002_h_000090

Cited by 2 publications

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“…The ability to detect analytes such as DNA, proteins, or other biologically active molecules at small concentrations and in the presence of a complicated matrix or even in vivo remains a challenging task. Many of these bioassays often rely on lanthanide fluorescence, which can furnish detection limits in the nano and picomolar region .…”

Section: Introductionmentioning

confidence: 99%

Highly Luminescent Lanthanide Complexes of 1-Hydroxy-2-pyridinones

et al. 2008

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The synthesis, X-ray structure, stability, and photophysical properties of several trivalent lanthanide complexes formed from two differing bis-bidentate ligands incorporating either alkyl or alkyl ether linkages and featuring the 1-hydroxy-2-pyridinone (1,2-HOPO) chelate group in complex with Eu(III), Sm(III) and Gd(III) are reported. The Eu(III) complexes are among some of the best examples, pairing highly efficient emission ( Eu tot Φ ~ 21.5 %,) with high stability (pEu ~ 18.6) in aqueous solution, and are excellent candidates for use in biological assays. A comparison of the observed behavior of the complexes with differing backbone linkages shows remarkable similarities, both in stability and photophysical properties. Low temperature photophysical measurements for a Gd(III) complex were also used to gain insight into the electronic structure, and were found to agree with corresponding TD-DFT calculations for a model complex. A comparison of the high resolution Eu(III) emission spectra in solution and from single crystals also revealed a more symmetric coordination geometry about the metal ion in solution due to dynamic rotation of the observed solid state structure.

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Section: Introductionmentioning

confidence: 99%

Highly Luminescent Lanthanide Complexes of 1-Hydroxy-2-pyridinones

et al. 2008

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“…The use of fluorescent assays is widespread and essential for the detection of a range of analytes, such as DNA, proteins, or other biologically active molecules typically present at very low concentrations (e.g., nano- or picomolar) and often in the presence of a complicated matrix of other chemical species (e.g., whole blood, serum, saliva). Due to their specific luminescent properties (sharp emission bands, large Stokes shift, and long-lived luminescence), lanthanide cations, such as Eu(III), are attractive as reporters for these assays. , Fluorescence detection is an exquisitely sensitive analytical tool, rapidly approaching the single molecule level .…”

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confidence: 99%

“Cymothoe sangaris”: An Extremely Stable and Highly Luminescent 1,2-Hydroxypyridinonate Chelate of Eu(III)

et al. 2006

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The synthesis, structure, and characterization of a new class of luminescent agents based on the 1,2-hydroxypyridinone chelator are reported. The prototype complex, [Eu(5LIO-1,2-HOPO)2]-, demonstrates superb aqueous stability [pEu = 18.64(10)] and is highly emissive [Phit = 0.21(3)], emitting almost pure "red" light (lambdaem = 612 nm). The crystal structure reveals a coordination geometry reminiscent of a "butterfly", hence the soubriquet of "Cymothoe sangaris" to identify with the unique species of red butterfly found only in Central Africa.

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Investigations on the binding of SYBR Green I to double-stranded (ds)DNA

Cited by 2 publications

References 0 publications

Highly Luminescent Lanthanide Complexes of 1-Hydroxy-2-pyridinones

Highly Luminescent Lanthanide Complexes of 1-Hydroxy-2-pyridinones

“Cymothoe sangaris”: An Extremely Stable and Highly Luminescent 1,2-Hydroxypyridinonate Chelate of Eu(III)

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