Investigations on the effects of ginsenoside-Rg1 on glucose uptake and metabolism in insulin resistant HepG2 cells

Fan, Xiaoming; Tao, Junyan; Zhou, Yulin; Hou, Yunhe; Wang, Yiyu; Gu, Danshan; Su, Yan-mei; Jang, Yanping; Li, Shude

doi:10.1016/j.ejphar.2018.11.024

Cited by 34 publications

(19 citation statements)

References 31 publications

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“…Then, the cells were continuously cultured for 30 min in the incubator and washed with PBS for two times, the cells were collected and filtered with 200 using mesh screen. Then the intracellular ROS levels were determined by flow cytometry according to our previous research methods (Fan et al, 2019b).…”

Section: Dcfh-da Staining Combined With Flow Cytometry Assaymentioning

confidence: 99%

Skimmin Improves Insulin Resistance via Regulating the Metabolism of Glucose: In Vitro and In Vivo Models

Zhang

Cai

et al. 2020

Front. Pharmacol.

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Skimmin is the major pharmacologically active component present in Hydrangea paniculata, in the traditional Chinese medicine as an anti-inflammatory agent, and its anti-inflammation and anti-diabetic effect has had been studied in previous studies. The metabolism of glucose plays an important role in the pathophysiology of diabetes. Therefore, it was identified as an important target for improving diabetic. Herein, we found that skimmin relieved the palmitic acid and high-fat and high sugar-induced insulin resistance. Furthermore, skimmin enhanced the glucose uptake via inhibiting reactive oxygen species (ROS) and reducing the level of inflammatory correlation factor. Meanwhile, skimmin reduced the glucose output by promoting PI3K/Akt signaling pathway and down-regulating the expression of glycogen synthase kinase-3b (GSK3b) and glucose-6-phosphatase (G6Pase). In conclusion, skimmin can improve the insulin resistance by increasing glucose uptake and decreasing glucose output in vitro and in vivo.

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Section: Dcfh-da Staining Combined With Flow Cytometry Assaymentioning

confidence: 99%

Skimmin Improves Insulin Resistance via Regulating the Metabolism of Glucose: In Vitro and In Vivo Models

Zhang

Cai

et al. 2020

Front. Pharmacol.

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“…In this study, HepG2 cells were selected to establish the insulinresistant cell model. Referring to relevant literature (Fan et al, 2019;Liu et al, 2018), four concentrations of insulin (10 −5 , 10 −6 , 10 −7 , 10 −8 mol/L) and three action times (24 hr, 36 hr, 48 hr) were set up to select the optimal concentration and time on the model of insulinresistant in HepG2 cells in the experiment. By comparing the glucose consumption rate and cell survival rate in each model group with those in the normal group, it was concluded that HepG2 cells treated with 10 −7 mol/L of insulin for 36 hr was the best condition to establish the model of insulin-resistant cells.…”

Section: Discussionmentioning

confidence: 99%

Limonoids isolated from fruits of Swietenia macrophylla king enhance glucose consumption in insulin‐resistant HepG2 cells via activating PPARγ

et al. 2021

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The fruits of Swietenia macrophylla King have been processed commercially to a variety of health foods and healthcare products and exhibited antidiabetic, antiinflammatory, antimutagenicity, antitumor activity, and so on. This study was aimed to examine the glucose consumption in human hepatoma HepG2 cells and the expression of PPARγ of limonoids isolated from the fruits of S. macrophylla.The phytochemical investigation of the fruits led to the isolation of ten limonoids which structures were elucidated by spectroscopic analysis as swietenine (1), khayasin T (2), 6-deoxyswietenine (3), 3-O-tigloylswietenolide (4), swietenolide (5), 3,6-O,O-diacetylswietenolide (6), 7-deacetoxy-7-oxogedunin (7), fissinolide (8), proceranolide (9), 7-deacetoxy-7α-hydroxygedunin (10), and compound 10 was isolated from this plant for the first time. The glucose consumption assay revealed that compounds 1, 2, 3, 5, and 9 could promote glucose consumption significantly in normal hyperglycemia-induced HepG2 cells, furthermore, compounds 1, 5, and 9 had a better effect on promoting glucose consumption in insulin-resistant HepG2 cells. In addition, compounds 1 and 5 could dramatically enhance the expression of PPARγ protein in insulin-resistant HepG2 cells according to the western blotting analysis result.How to cite this article: Duan J-Y, Wang Y-J, Chen W, et al.Limonoids isolated from fruits of Swietenia macrophylla king enhance glucose consumption in insulin-resistant HepG2 cells via activating PPARγ.

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“…When insulin resistance occurs, the glucose metabolism of the liver is disordered, and the uptake and utilization of glucose in liver will be reduced. At the same time, the synthesis of glycogen is inhibited, and the endogenous glycogen is partially decomposed (Fan et al., 2019). In the present research, HepG2 cells were used to establish an insulin resistance model to further study the hypoglycemic effect of DRE‐w, RAE, and their combination in vitro.…”

Section: Discussionmentioning

confidence: 99%

Hypoglycemic effect of Taraxacum officinale root extract and its synergism with Radix Astragali extract

Chai

Guo

et al. 2021

Food Science & Nutrition

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Taraxacum officinale (dandelion) and Radix Astragali are traditional medicinal and edible plants with high nutritional value. In this study, the synergistic hypoglycemic effect of DRE and Radix Astragali extract (RAE) was evaluated. Our results showed that water extract of dandelion (DRE‐w), mainly containing polysaccharides (63.92 ± 1.82 mg/g), total flavonoid (2.57 ± 0.06 mg/g), total phenolic compounds (8.93 ± 0.34 mg/g), and saponins (0.54 ± 0.05 mg/g), exhibited significantly inhibitory effect on α‐glucosidase and α‐amylase. DRE‐w and RAE had synergistic hypoglycemic effect; we found that DRE‐w and its combination with RAE could relieve the state of insulin resistance in IR‐HepG2 cells. The combination could more significantly increase the glucose consumption and intracellular glycogen content, and improve the activity of hexokinase and pyruvate kinase in IR‐HepG2 cells. In summary, DRE and its combination with RAE can be developed as the drugs or functional foods for diabetes prevention and treatment.

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Investigations on the effects of ginsenoside-Rg1 on glucose uptake and metabolism in insulin resistant HepG2 cells

Cited by 34 publications

References 31 publications

Skimmin Improves Insulin Resistance via Regulating the Metabolism of Glucose: In Vitro and In Vivo Models

Skimmin Improves Insulin Resistance via Regulating the Metabolism of Glucose: In Vitro and In Vivo Models

Limonoids isolated from fruits of Swietenia macrophylla king enhance glucose consumption in insulin‐resistant HepG2 cells via activating PPARγ

Hypoglycemic effect of Taraxacum officinale root extract and its synergism with Radix Astragali extract

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