Investigations on the influence of duodenal histidine infusion on nitrogen and amino acid turnover of growing German holstein bulls

Abstract

The effect of a continuous duodenal infusion of L-histidine (His) (8 g/d) on the retention of nitrogen was investigated in two experiments (I, II), each of which was carried out using two young bulls. In Exps. I and II, the animals (150-250 kg BW) were fitted with a re-entrant cannula in the proximal duodenum and were fed diets containing 125 g CP/kg DM and 11.5 MJ ME/kg DM. A third experiment (III) using two young bulls (140-200 kg BW) fitted with a simple T-cannula was carried out infusing 6 g L-His. The animals were fed a low protein diet (94 g CP/kg DM and 11 MJ ME/kg DM). The study was done to find out whether or not L-His is the first limiting amino acid (AA) for growing ruminants. N retention was 28 and 31, 38 and 38, 22 and 24 g/d without L-His infusion and with L-His infusion for Exps. I, II and III, respectively. Both in the experiments with a standard protein supply (I, II) and in the experiment with reduced protein supply (III), no significant differences were found between periods with and without infusion of L-His. The utilisation of duodenal NAN varied between 39% and 50% and was also not significantly influenced by the duodenal infusion of L-His. No significant effect was observed on the flow of AA into the duodenum. The faecal excretion of AA was also not significantly influenced by the infusion of L-His. The utilisation of individual amino acids as calculated by the ratio of retained AA to intestinal apparently digested AA, did not differ significantly following the duodenal infusion of L-His. As expected, the utilisation of His decreased. Of the different essential AA, L-His was the most utilised (80%) followed by Arg (72%), Met (60%), Leu (45%) and Lys (44%), during periods without supplementation of L-His. It is concluded that the intestinal supply of L-His from the basal diet was sufficient for the potential growth level of animals under these experimental conditions. In all AA present at the proximal duodenum, L-His could have at first a limiting effect on the performance of growing young bulls with high body gain. Arg and Met, but not Lys, could be second or co-limiting AA.

Histidine: A Systematic Review on Metabolism and Physiological Effects in Human and Different Animal Species

“…The study concluded that histidine from the basal diet was sufficient to maintain growth under the experimental conditions employed. Nevertheless, in all AA present in the duodenum, histidine could be the first limiting AA for growth [108]. Similarly, a study by Oldham [109] also concluded that histidine was the first limiting AA in raising cattle with maize silage.…”

“…Schoof et al [108] explored whether histidine is the first limiting AA for growing ruminants. As such, they used a canula to evaluate the effects of continuous duodenal infusion of histidine on the retention of nitrogen and AA (AA) utilization.…”

Safety and efficacy of l‐histidine monohydrochloride monohydrate produced by fermentation with Escherichia coli (NITE BP‐02526) for all animal species

“…Proteins and histidine dipeptide (carnosine and anserine) content in the muscles increased by diet supplemented with histidine. Schoof et al (2000) evaluated the effects of continuous duodenal infusion of L-histidine on the retention of nitrogen and amino acids utilisation in young bulls. No significant differences were observed following histidine infusion compared to the control.…”

Verwertung der im Intestinum wachsender Bullen absorbierten essentiellen Aminosäuren

“…Diese Schwierigkeiten erklären die große Schwankungsbreite dieser Größe von 50 bis 80 % in verschiedenen Proteinbewertungssystemen für Wiederkäuer (ALDERMAN, 1987). Die Mehrzahl der Autoren, die unterschiedlichste experimentelle Methoden mit dem Ziel der Bestimmung dieses Parameters anwandten, gehen vorwiegend auf Untersuchungen mit Schafen und Kühen zurück (STORM und 0RSKOV, 1982;SMITH, 1984;WHITELAW et al, 1986;OLDHAM, 1987;0RSKOV und CHEN, 1989). Eine Literaturzusammenstellung von ROHR und LEBZIN (1991) ergab eine Verwertung des AA-N für die Milchproduktion von 75 bis 80 %.…”

“…Ausnahmen bilden das His und Met, die trotz einer weiteren Abnahme der Verwertung 2 bei einem Ernährungsniveau von EB • 2 im Gegensatz zu den übrigen AA weiterhin eine Verwertung von über 100 % aufweisen. Zu ähnlich hohen Verwertungsraten von über 100 % beim His und Met gelangtenSCHOOF et al (2000) bei Untersuchungen an wachsenden Jungbullen in einem Le-bis 200 kg. Aus Untersuchungen vonGABEL und POPPE (1988), die die Verwertung der einzelnen essentiellen scheinbar verdaulichen AA in Abhängigkeit vom Ernährungsniveau (Vielfache des energetischen Erhaltungsbedarfes) ermittelten, ergab sich gleichfalls eine abnehmende Verwertung dieser AA mit zunehmendem Ernährungsniveau, wobei His gefolgt von Lys und Leu die höchsten Verwertungsraten aufwiesen.…”