1983
DOI: 10.1080/17450398309425668
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Investigations on the pancreatic and stomach secretion in pigs by means of continuous infusion of14C-amino acids

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Cited by 39 publications
(12 citation statements)
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“…It has been reported that up to 25% of the daily protein synthesis in the body may be secreted into the gut (Simon et al., 1983). Endogenous nitrogen consists mainly of nitrogen from digestive enzymes, mucoproteins, desquamated cells, serum albumin, peptides, free amino acids and amines (Moughan and Schuttert, 1991).…”
Section: Introductionmentioning
confidence: 99%
“…It has been reported that up to 25% of the daily protein synthesis in the body may be secreted into the gut (Simon et al., 1983). Endogenous nitrogen consists mainly of nitrogen from digestive enzymes, mucoproteins, desquamated cells, serum albumin, peptides, free amino acids and amines (Moughan and Schuttert, 1991).…”
Section: Introductionmentioning
confidence: 99%
“…Taking into account that both extracellular amino acids originating from plasma or digesta and intracellular amino acids derived from intracellular protein degradation are used for protein synthesis (Simon et al, 1982(Simon et al, , 1983 and that endogenous N in the gut lumen is derived from multiple precursor pools (Moughan et al, 1992), the ,5 N-enrichment in urinary N was used as an indicator of that in endogenous N during a steady state after withdrawal of 15 NH 4 C1. At this time, the 15 N in urine originated only from the catabolism of 15 N-labelled body protein.…”
Section: Methodical Aspectsmentioning
confidence: 99%
“…The continuous-infusion method (Waterlow et al 1978 a) requires several hours to reach a plateau of constant labelling, even in the rat, and has largely been replaced by giving a flooding dose of tracer amino acid, which produces uniform labelling of free amino acid over 10-20 min (Kean, 1959;Scornik & Botbol, 1976;McNurlan, Tomkins & Garlick, 1979;Garlick, McNurlan & Preedy, 1980 c). The method is particularly useful for rapidly turning over tissues such as gut mucosa (McNurlan, Pain & Garlick, 1980) and pancreas (Simon, Zebrowska, Bergner & Munchmeyer, 1983). Because of the short time scale, the method measures the synthesis of both fixed and secreted proteins.…”
Section: Measurement Ofprotein Turnover Rates In Individual Tissuesmentioning
confidence: 99%