Investigations on the relationship between DNA ethenobase adduct levels in several organs of vinyl chloride-exposed rats and cancer susceptibility

Ciroussel, Françoise; Barbin, Alain; Eberle, Gertrud; Bartsch, Helmut

doi:10.1016/0006-2952(90)90291-r

Cited by 32 publications

(25 citation statements)

References 17 publications

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“…7-(2'-Oxoethyl)guanine and three etheno adducts (-A; £C; and N2,3-EG; Figure 2) have been detected in DNA from rats and mice exposed to VC (123,(128)(129)(130)(131). Highly variable background levels ofcA and -C were found in all the tissues examined (132,133).…”

Section: Vc Initiation Of Hepatocarcinogenesismentioning

confidence: 93%

Vinyl Chloride: Still a Cause for Concern

Kielhorn¹,

Melber²,

Wahnschaffe³

et al. 2000

Environmental Health Perspectives

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Section: Vc Initiation Of Hepatocarcinogenesismentioning

confidence: 93%

Vinyl Chloride: Still a Cause for Concern

Kielhorn¹,

Melber²,

Wahnschaffe³

et al. 2000

Environmental Health Perspectives

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“…For exposure to VC, animals were placed in an inhalation chamber with an airflow of ~15 chamber volume changes per hour, as described previously (22). VC concentration at four different positions inside the chamber was monitored hourly by gas chromatography on Porapack QS (80-100 mesh, from Waters Associates, Milford, MA), with flame ionisation detection.…”

Section: Vc Exposurementioning

confidence: 99%

“…The analysis by HPLC/RIA was further used to measure edAdo and edCyd in DNA samples from various tissues (liver, lung, brain) of 7-day old and from liver of 13-week old BD VI rats exposed to 500 p.p.m. VC, 7 h/day during 2 weeks (22). Finally, a comparative study on the formation and persistence of the four VC-DNA adducts was carried out with 10-day old Sprague-Dawley rats exposed to 600 p.p.m.…”

Section: Introductionmentioning

confidence: 99%

“…7-(2-Oxoethyl)-guanine and the ethenobases were quantitated in the liver, lung and kidney: all three etheno adducts appeared to be persistent in liver DNA 2 weeks after the end of exposure (20). At that time, a similar study on adult animals was not feasible because of the lower levels of DNA alkylation produced by VC in adult, as compared to young rats (19,22,23).…”

Section: Introductionmentioning

confidence: 99%

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ACCELERATED PAPER: Formation and accumulation of DNA ethenobases in adult Sprague-Dawley rats exposed to vinyl chloride

Guichard¹,

Ghissassi²,

Nair³

et al. 1996

Carcinogenesis

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DNA ethenobases are promutagenic lesions formed by carcinogens such as vinyl chloride (VC). Their formation was investigated in 6-week old, male Sprague-Dawley rats exposed to 500 p.p.m. VC by inhalation (4 h/day, 5 days/ week) for 1, 2, 4 or 8 weeks and in 7- and 14-week old, matched control animals. 1,N6-Ethenoadenine (epsilon A) and 3, N4-ethenocytosine (epsilon C) deoxyribonucleotides were analysed by immunoaffinity purification and 32P-postlabelling. This postlabelling method was compared with a radio-immunoassay method, which yielded similar results. Background levels of ethenobases were found in DNA from the liver, lungs, kidneys and circulating lymphocytes of unexposed, control rats. In the liver, the following background molar ratios of ethenobase to parent base in DNA were detected (mean values x 10(-8)): epsilon A/A, 0.04-0.05; epsilon C/C, 0.06-0.07. In the lungs, kidneys and circulating lymphocytes, background levels of epsilon A and epsilon C ranged from 1.7 to 4.2 x 10(-8) and from 4.8 to 11.2 x 10(-8), respectively. Following a 5-day exposure to VC, a significant increase of epsilon A and epsilon C was measured in hepatic DNA from rats sacrificed immediately after treatment. Further, a dose-dependent increase of both etheno adducts was observed in liver DNA of VC-treated rats. Compared to the 5-day exposure, approximately 4-fold higher levels of epsilon A and epsilon C were observed in the liver of animals after 8 weeks of exposure. In contrast, there was an accumulation of epsilon C but not of epsilon A in lungs and kidneys. In circulating lymphocytes, no significant increase of ethenobase levels above control values was observed after 2 months of exposure to VC. Both etheno adducts were found to be persistent in liver DNA, after 2 months following the termination of VC exposure. These results further support the notion that DNA etheno-bases are critical lesions in VC-induced carcinogenesis. The possible contribution of lipid peroxidation products that also yield ethenobases, on the formation and persistence of these DNA adducts, remains to be clarified.

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“…The role of DNA etheno adducts in mutagenic and carcinogenic processes triggered by known occupational and environmental carcinogens, such as vinyl chloride and ethyl carbamate, has been well investigated (Foiles et al, 1993;. It has been shown that some of these lesions accumulate in DNA after chronic carcinogen exposure, leading to miscoding upon replication or transcription (Swenberg et al, 1992 been detected in different tissues of vinyl chloride exposed rats (Eberle et al, 1989;Ciroussel et al, 1990). Interestingly, etheno adducts have also been detected as background lesions in tissues of rodents and humans not exposed to carcinogens (Chung et al, 1996;.…”

Section: Introductionmentioning

confidence: 99%

Avaliação de variações bioquímicas em moluscos bivalves em resposta ao estresse ambiental

Almeida¹

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In this work, we report direct evidence of the formation of singlet molecular oxygen [O 2 (1 g)] in the reaction of hydrogen peroxide (H 2 O 2) with acetonitrile in alkaline solutions. The formation of O 2 (1 g) was characterized by: (i) the dimol light emission in the red spectral region (>610 nm) using a red-sensitive photomultiplier tube (PMT); (ii) the monomol light emission in the near-infrared region (1270 nm) with a newly available tube coupled to a monochromator; and (iii) the quenching effect of sodium azide. Direct spectral characterization of the near-infrared emission that attributes the emission to the transition of O 2 (1 g) to the triplet ground state (O 2 (3 − g)) was done to unequivocally demonstrate the presence of O 2 (1 g). For comparison, O 2 (1 g) derived from the thermolysis of the endoperoxide of 1,4-dimethylnaphthalene or from the H 2 O 2 /hypochlorite system were also monitored. The product of the reaction (acetamide) was also detected by mass spectrometry (MS). This evidence clearly demonstrates that the reaction of H 2 O 2 with acetonitrile in alkaline solutions generates O 2 (1 g), confirming the mechanism proposed by McKeown and Waters.

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Investigations on the relationship between DNA ethenobase adduct levels in several organs of vinyl chloride-exposed rats and cancer susceptibility

Cited by 32 publications

References 17 publications

Vinyl Chloride: Still a Cause for Concern

Vinyl Chloride: Still a Cause for Concern

ACCELERATED PAPER: Formation and accumulation of DNA ethenobases in adult Sprague-Dawley rats exposed to vinyl chloride

Avaliação de variações bioquímicas em moluscos bivalves em resposta ao estresse ambiental

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