Investigations on the specificity of hormone receptors in planarians

Csaba, G.; Bierbauer, J

doi:10.1016/0016-6480(74)90095-1

Cited by 17 publications

(4 citation statements)

References 3 publications

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“…As observed previously (Ireland et al 2022), lesions appear to be an early indicator of systemic toxicity. Histamine (180 µM) has been found to induce morphological distortions (primarily supernumerary eyes) in regenerating trunk and tail fragments of Dugesia lugubris (Csaba and Bierbauer 1974). We did not observe any effects on eye regeneration at up to 100 µM histamine, though we did find an increase in c-shapes and contraction on day 12 in 100 µM histamine-exposed regenerating D. japonica.…”

Section: Hts Can Recapitulate Findings From Low-throughput Studiescontrasting

confidence: 71%

Adult and regenerating planarians respond differentially to chronic drug exposure

Bayingana

Ireland

Rosenthal

et al. 2023

Neurotoxicology and Teratology

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Section: Hts Can Recapitulate Findings From Low-throughput Studiescontrasting

confidence: 71%

Adult and regenerating planarians respond differentially to chronic drug exposure

Bayingana

Ireland

Rosenthal

et al. 2023

Neurotoxicology and Teratology

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“…In another study, it was shown that some animal polypeptide hormones have binding sites on the surface of unicellular organisms. However, these hormones are not naturally found in these living organisms or in their growth media, and may have appeared on the membrane surface during evolution of the species [51,52]. The most interesting difference between the effect of insulin one other micro or macroorganisms and its effect on algae is the impact of insulin on the presence of sugars such as glucose in the medium, because the algal medium used did not include any type of sugar or any other carbon sources except for EDTA, which was used only as a chelating agent by algae [3].…”

Section: Resultsmentioning

confidence: 98%

Physiological studies on microalgal culture additives to optimize growth rate and oil content

Gomaa

Hafez²,

Lim

et al. 2011

Bioprocess Biosyst Eng

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Insulin, in nature, has a stimulatory effect on microorganisms. These effects include the acceleration of sugar metabolism, triacylglycerol anabolism, growth rate, and formation of oils. We also observed that insulin may cause indirect activation of triacylglycerol lipase by forcing the cell to permanently require an energy source. Thus, cells can consume all of their accumulated internal fuel sources such as lipids, proteins, and carbohydrates. After studying the effects of using two types of insulin (Humulin 70/30, and human insulin expressed in yeast) at different concentrations on microalgae (Chlorella sp.), we found that with certain concentrations of insulin (1:3.3 ml unit Humulin 70/30 per ml; 1:2.6 ml unit yeast insulin per ml), there was an increase in algal growth rate and decrease in cell size. We therefore studied the effect of insulin under conditions of lipase inhibition by Triton WR 1339 (Tyloxapol), which was used at different concentrations with and without insulin. We found strong regression in the growth rate with increasing Triton concentrations. However, we also observed that the cell size under the effect of Triton and Triton-insulin was larger than the cell size under the effect of insulin alone, and also larger than for control cells. Also, the oil content of the Triton-insulin cells was higher than those of the control cells or the cells under the effect of insulin alone.

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Section: Discussionmentioning

confidence: 99%

Adult and regenerating planarians respond differentially to chronic drug exposure

Bayingana

Ireland

Rosenthal

et al. 2022

Preprint

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There is a lack of data on the effects of chronic exposure to common drugs and stimulants on the developing nervous system. Freshwater planarians have emerged as a useful invertebrate model amenable to high-throughput behavioral phenotyping to assay chemical safety in adult and developing brains. Here, we leverage the unique strength of the system to test in parallel for effects on the adult and developing nervous system, by screening ten common drugs and stimulants (forskolin, clenbuterol, LRE-1, MDL-12,330A, adenosine, caffeine, histamine, mianserin, fluoxetine and sertraline) using the asexual freshwater planarian Dugesia japonica. The compounds were tested up to 100 μM nominal concentration for their effects on planarian morphology and behavior. Quantitative phenotypic assessments were performed on days 7 and 12 of exposure using an automated screening platform. The antidepressants sertraline and fluoxetine were the most potent to induce lethality, with significant lethality observed at 10 μM. All ten compounds caused sublethal morphological and/or behavioral effects, with the most effects, in terms of potency and breadth of endpoints affected, seen with mianserin and fluoxetine. Four of the compounds (forskolin, clenbuterol, mianserin, and fluoxetine) were developmentally selective, causing effects at lower concentrations in regenerating planarians. Of these, fluoxetine showed the greatest differences between the two developmental stages, inducing many behavioral endpoints in regenerating planarians but only a few in adult planarians. While some of these behavioral effects may be due to neuroefficacy, these results substantiate the need for better evaluation of the safety of these common drugs on the developing nervous system.

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Investigations on the specificity of hormone receptors in planarians

Cited by 17 publications

References 3 publications

Adult and regenerating planarians respond differentially to chronic drug exposure

Adult and regenerating planarians respond differentially to chronic drug exposure

Physiological studies on microalgal culture additives to optimize growth rate and oil content

Adult and regenerating planarians respond differentially to chronic drug exposure

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