Investigations on vinylene carbonate. VI. Immobilization of alkaline phosphatase onto poly(vinylene carbonate) ‐jeffamine® hydrogel beads

Abstract

SYNOPSISHydrogel-like polymers with reactive cyclic carbonate ( CCA) groups have been obtained by the cross-linking reaction of poly(viny1ene carbonate) (PVCA) with Jeffamines@. By this reaction, microspheric hydrogel beads with a high water content and a high concentration of reactive CCA groups were prepared. The beads were used as a matrix for the immobilization of the enzyme alkaline phosphatase (ALP) and showed a considerable capacity to couple ALP and a reasonable retention of activity for the immobilized ALP, depending on the reaction conditions. The immobilized ALP exhibited a better thermal stability than did native ALP and a high residual activity was found after repeated use.

The Use of Transesterification Method for Obtaining Phosphorus‐Containing Polymers

“…9,26,[32][33][34] The synthesis of reactive polymers is one of the most challenging, interesting, and important areas in the research of polymer science. 54,55 Poly(oxyethylene H-phosphonate) 5 was synthesized following the same procedure shown in Fig. In such a way, several important drugs and biopharmaceuticals could be obtained under mild reaction conditions.…”

“…45 A new class of biodegradable poly(alkylene-Hphosphonate)s was obtained from the conversion of poly(alkylene phosphate)s. 3,[39][40][41][46][47][48][49][50][51][52] The 1,3-dioxolan-2-one ring displays high chemoselective reactivity toward aliphatic amines 53 and it can be used for immobilization of enzymes and drugs. 54,55 Poly(oxyethylene H-phosphonate) 5 was synthesized following the same procedure shown in Fig. 4 from dimethyl Hphosphonate and poly(ethylene glycol).…”

“…7). 26,27,54,55 Koseva et al proved that a common precursor polymer can be transformed into three different PPEs by different reactions. 25 On the other hand, the synthesis of a triblock copolymer (Fig.…”

Synthesis of poly(isopropenylphenoxy propylene carbonate) and its facile side‐chain functionalization into hydroxy‐polyurethanes

“…The non‐isocyanate route (NIR) for production of polyurethanes (PUs) using ring‐opening reaction of polycyclic carbonates with diamines has been touted as one of the most potentially useful green chemistry routes to PUs in recent years . This ring‐opening reaction involving a cyclic carbonate with aliphatic amines will result in β‐hydroxyls as side‐groups on urethane main chains, and thus this route has been applied to synthesize hydrophilic PUs . However, this NIR process generally produces PUs with low molecular weights and low T g which have hampered its usefulness in applications.…”

“…Synthesis of highly functionalized cyclic carbonate prepolymers through polymerization of vinyl‐monomers bearing cyclic carbonates have been reported as yet another NIR‐strategy of making crosslinked polyurethanes as means of enhancing physical properties. In 1993, G. Chen et al prepared PU‐hydrogel beads for immobilization of enzymes by reacting poly(vinylene carbonate) with difunctional Jeffamines. More recently, several other groups carried out homo and copolymerized of acrylic and styrenic intermediates possessing cyclic carbonates.…”