Invitro regulation of RNA polymerase in estrogen-treated uteri

Nicolette, John A.; Mueller, Gerald C.

doi:10.1016/0006-291x(66)90326-3

Cited by 47 publications

(7 citation statements)

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“…This explanation was found to account for the increased activity of DNA-dependent RNA polymerase observed in rat uterus after oestrogen administration (Noteboom & Gorski, 1963 ;Nicolette & Mueller, 1966) or in rat liver after partial hepatectomy (Tsukada & Lieberman, 1964). This possibility was therefore tested by pretreating animals with cycloheximide or puromycin, feeding with the T+ diet and killing the animals 15min later.…”

Section: Resultsmentioning

confidence: 96%

The effect of feeding with a tryptophan-free amino acid mixture on rat liver magnesium ion-activated deoxyribonucleic acid-dependent ribonucleic acid polymerase

Henderson

1970

Biochemical Journal

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1. The Widnell & Tata (1966) assay method for Mg(2+)-activated DNA-dependent RNA polymerase was used for initial-velocity determinations of rat liver nuclear RNA polymerase. One unit (U) of RNA polymerase was defined as that amount of enzyme required for 1 mmol of [(3)H]GMP incorporation/min at 37 degrees C. 2. Colony fed rats were found to have a mean RNA polymerase activity of 65.9muU/mg of DNA and 18h-starved rats had a mean activity of 53.2muU/mg of DNA. Longer periods of starvation did not significantly decrease RNA polymerase activity further. 3. Rats that had been starved for 18h were used for all feeding experiments. Complete and tryptophan-deficient amino acid mixtures were given by stomach tube and the animals were killed 15-120min later. The response of RNA polymerase to the feeding with the complete amino acid mixture was rapid and almost linear over the first hour of feeding, resulting in a doubling of activity. The activity was still elevated above the starvation value at 120min after feeding. The tryptophan-deficient amino acid mixture produced a much less vigorous response about 45min after the feeding, and the activity had returned to the starvation value by 120min after the feeding. 4. The response of RNA polymerase to the feeding with the complete amino acid mixture was shown to occur within a period of less than 5min to about 10min after the feeding. 5. Pretreatment of the animals with puromycin or cycloheximide was found to abolish the 15min RNA polymerase response to the feeding with the complete amino acid mixture, but the activity of the controls was unaffected. 6. The characteristics of the RNA polymerase from 18h-starved animals and animals fed with the complete or incomplete amino acid mixtures for 1h were examined. The effects of Mg(2+) ions, pH, actinomycin D and nucleoside triphosphate omissions were determined. The [Mg(2+)]- and pH-activity profiles of the RNA polymerase from the animal fed with the complete mixture appeared to differ from those of the enzyme from the other groups, but this difference is probably not significant. 7. [5-(3)H]Orotic acid incorporation by rat liver nuclei in vivo was shown to be affected by the amino acid mixtures in a similar manner to the RNA polymerase. 8. The tryptophan concentrations of plasma and liver were determined up to 120 min after feeding with the amino acid mixtures. Feeding with the complete mixture produced a rapid increase in free tryptophan concentrations in both plasma and liver, but feeding with the incomplete mixture did not alter the plasma concentration. The liver tryptophan concentration increased at about 45min after feeding with the tryptophan-deficient diet. 9. There was a good correlation between the liver tryptophan concentration and RNA polymerase activity in all groups of animals. 10. It was concluded that the rat liver nucleus responded to an increase in amino acid supply by increased synthesis of RNA as a result of synthesis of RNA polymerase de novo. The correlation of tryptophan concentration and RNA polymerase activi...

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Section: Resultsmentioning

confidence: 96%

The effect of feeding with a tryptophan-free amino acid mixture on rat liver magnesium ion-activated deoxyribonucleic acid-dependent ribonucleic acid polymerase

Henderson

1970

Biochemical Journal

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“…It has been shown that the biological action of oestrogens in immature rat uterus is preceded by increased uptake of labelled precursors into RNA (Gorski & Nicolette, 1963) and that the DNAdependent RNA polymerase (EC 2.7.7.6) activity of the uterus also increases (Gorski, 1964;Nicolette & Mueller, 1966). There is also evidence that increased labelling of RNA occurs in the uterus of the adult ovariectomized rat in response to oestrogen treatment (Means & Hamilton, 1966;Teng & Hamilton, 1967).…”

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confidence: 99%

Changes in the patterns of synthesis of ribonucleic acid species in immature rat uterus in response to oestradiol-17β

Billing

Barbiroli

Smellie

1969

Biochemical Journal

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1. After treatment of immature rats with diethylstilboestrol, the wet weight and RNA content of uterine tissue increased rapidly, reaching a peak at 40hr. After an initial lag of a few hours, the acid-soluble ribose and protein contents also rose to maxima at 40hr. No increase in DNA content occurred until at least 24hr. after treatment. 2. The RNA from immature rat uterus isolated at various times up to 6hr. after administration of oestradiol-17beta was labelled by injecting [(3)H]uridine and [(3)H]guanosine intraperitoneally 30min. before the animals were killed. It was fractionated on columns of kieselguhr coated with methylated serum albumin and the radioactivity in fractions corresponding to transfer RNA, 7s RNA, ribosomal RNA, Q(1)-RNA, Q(2)-RNA and DNA-like RNA was determined. 3. The radioactivity of the whole RNA increased steadily for 6hr. after hormone treatment. The earliest changes occurred in the Q(1)-RNA (ribosomal RNA precursor), whereas at longer time-intervals the radioactivity of the ribosomal RNA, 7s RNA and transfer RNA increased by four- to five-fold. The radioactivity of the DNA-like RNA increased by about 50%, but only at the longer time-intervals. 4. It is concluded that one of the earliest changes in response to oestradiol treatment is a major increase in synthesis of ribosomal RNA followed later by a similar increase in synthesis of transfer RNA and by a much smaller increase in synthesis of DNA-like RNA. The change in synthesis of ribosomal RNA in immature rat uterus may represent one of the most important responses to oestradiol treatment.

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“…The time course studies indicate that PGDH is a very short-lived enzyme with a half-life of approximately 1 h after cycloheximide injection. The life of proteins within cells may vary from less than I h to 20 days or more (Schapira, Kruh, Dreyfus & Schapira, 1960;Kenney, 1962;Nicolette & Mueller, 1966;Gorski & Morgan, 1967). The short life span of PGDH may be due to an inherent instability of the protein molecule.…”

Section: Discussionmentioning

confidence: 99%

“…The fact that we have obtained the same effect on kidney slices in vitro (Blackwell et al, 1975) & Schapira, 1960;Kenney, 1962;Nicolette & Mueller, 1966;Gorski & Morgan, 1967 What are the physiological implications of this work? PDGH belongs to that group of enzymes (which includes phosphodiesterase, acetylcholinesterase, monoamine-oxidase and kininase) responsible for the inactivation of endogenous substances of high biological activity (Marazzi & Andersen, 1974).…”

Section: Effect Of Cycloheximide On Pgdh In the Lungmentioning

confidence: 99%

Rapid Reduction of Prostaglandin 15‐hydroxy Dehydrogenase Activity in Rat Tissues After Treatment With Protein Synthesis Inhibitors

Blackwell

Flower

Vane

1975

British J Pharmacology

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1The capacity of rat kidneys to metabolize prostaglandins is rapidly lost when de novo protein synthesis is reduced by cycloheximide and puromycin. Enzyme activity declines to about half control values 45-75 min after a single dose of cycloheximide. Prostaglandin metabolism by the lung is also diminished. 2 These inhibitors apparently act by preventing the synthesis of new prostaglandin 15-hydroxy dehydrogenase enzyme. 3 The RNA synthesis inhibitor actinomycin D has no effect on metabolism. 4 It is concluded that prostaglandin dehydrogenase is a short-lived enzyme in the cell whose replacement depends upon de novo protein (but not RNA) synthesis. The implications of this finding are discussed.

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Invitro regulation of RNA polymerase in estrogen-treated uteri

Cited by 47 publications

References 7 publications

The effect of feeding with a tryptophan-free amino acid mixture on rat liver magnesium ion-activated deoxyribonucleic acid-dependent ribonucleic acid polymerase

The effect of feeding with a tryptophan-free amino acid mixture on rat liver magnesium ion-activated deoxyribonucleic acid-dependent ribonucleic acid polymerase

Changes in the patterns of synthesis of ribonucleic acid species in immature rat uterus in response to oestradiol-17β

Rapid Reduction of Prostaglandin 15‐hydroxy Dehydrogenase Activity in Rat Tissues After Treatment With Protein Synthesis Inhibitors

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