Involucrin in Laryngeal Dysplasia: A Marker for Differentiation

Kaplan, Michael; Mills, Stacey E.; Rice, Robert H.; Johns, Michael E.

doi:10.1001/archotol.1984.00800370015003

Cited by 17 publications

(5 citation statements)

References 11 publications

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“…Using microarray and TCGA transcriptomic datasets, mRNA expression levels of IVL and KRT13 were found to be significantly downregulated in OSCC/head and neck SCC as compared to normal controls (Figure 2(A,B)). Interestingly, their expression levels were similar in normal controls and oral dysplasia (Figure 2(A)), despite some previous studies that have shown a downregulation of CK13 or IVL in dysplastic lesions compared to normal controls 29–31 . This discrepancy could be due to different methods used for expression analysis or due to variation in the grade of dysplasia of the specimens used.…”

Section: Discussionmentioning

confidence: 70%

“…Interestingly, their expression levels were similar in normal controls and oral dysplasia (Figure 2(A)), despite some previous studies that have shown a downregulation of CK13 or IVL in dysplastic lesions compared to normal controls. [29][30][31] This discrepancy could be due to different methods used for expression analysis or due to variation in the grade of dysplasia of the specimens used. In contrast, the upregulation of CK10 mRNA already in oral dysplasia and OSCC/head and neck SCC (Figure 2(A,B)) suggests that its upregulation might be an early event in OSCC.…”

Section: Discussionmentioning

confidence: 99%

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Combined loss of expression of involucrin and cytokeratin 13 is associated with poor prognosis in squamous cell carcinoma of mobile tongue

et al. 2021

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Background This study aimed to evaluate the prognostic significance of expression levels of involucrin (IVL), cytokeratin (CK)‐10 and ‐13 at different intratumor sites (tumor center and invading area) of oral tongue squamous cell carcinoma (OTSCC). Methods IVL, CK13 and CK10 expression levels were examined in a multicenter cohort of 146 OTSCCs using immunohistochemistry. External mRNA datasets were used for expression analysis and/or to validate survival associations. Results External transcriptomic datasets showed downregulation of IVL and KRT13 in oral malignancies including OTSCC as compared to normal controls. The combined loss of IVL and CK13 expression at the invading core but not at the center core was significantly associated with poor differentiation and reduced 5‐year overall survival. Multivariate Cox analysis confirmed the loss of CK13 and IVL expression to be an independent prognostic factor. Transcriptomic dataset corroborated immunohistochemistry results. Conclusions Combined expression levlels of IVL and CK13 might be useful as prognostic biomarkers in OTSCC.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Combined loss of expression of involucrin and cytokeratin 13 is associated with poor prognosis in squamous cell carcinoma of mobile tongue

et al. 2021

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“…Within the CK13 + cell layers, however, the involucrin + subset of layers occurs at the luminal surface of the epithelium and likely represents the most mature differentiated squamous cells. 22,23 Indeed, involucrin is known to be expressed at the surface of stratified squamous epithelia of the epidermis, hard palate, and buccal mucosa. 24 Of note, an association between involucrin expression and well-differentiated tumors has been demonstrated in several studies.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, we find that CK13 and CK14 together reliably and reproducibly mark every one of the cells of the epithelium, but they overlap considerably. Within the CK13 + cell layers, however, the involucrin + subset of layers occurs at the luminal surface of the epithelium and likely represents the most mature differentiated squamous cells . Indeed, involucrin is known to be expressed at the surface of stratified squamous epithelia of the epidermis, hard palate, and buccal mucosa .…”

Section: Discussionmentioning

confidence: 99%

Identification of distinct layers within the stratified squamous epithelium of the adult human true vocal fold

et al. 2015

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Objectives/Hypothesis A precise molecular schema for classifying the different cell types of the normal human vocal fold epithelium is lacking. We hypothesize that the true vocal fold epithelium has a cellular architecture and organization similar to that of other stratified squamous epithelia including the skin, cornea, oral mucosa, and esophagus. In analogy to disorders of the skin and gastrointestinal tract, a molecular definition of the normal cell types within the human vocal fold epithelium and a description of their geometric relationships should serve as a foundation for characterizing cellular changes associated with metaplasia, dysplasia, and cancer. Study Design Qualitative study with adult human larynges. Methods Histologic sections of normal human laryngeal tissue were analyzed for morphology (hematoxylin and eosin) and immunohistochemical protein expression profile, including cytokeratins (CK13 and CK14), cornified envelope proteins (involucrin), basal cells (NGFR/p75), and proliferation markers (Ki67). Results We demonstrated that three distinct cell strata with unique marker profiles are present within the stratified squamous epithelium of the true vocal fold. We used these definitions to establish that cell proliferation is restricted to certain cell types and layers within the epithelium. These distinct cell types are reproducible across five normal adult larynges. Conclusion We have established that three layers of cells are present within the normal adult stratified squamous epithelium of the true vocal fold. Furthermore, replicating cell populations are largely restricted to the parabasal strata within the epithelium. This delineation of distinct cell populations will facilitate future studies of vocal fold regeneration and cancer. Level of Evidence N/A.

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“…Expression of involucrin, cytokeratins and of p21 WAF1 is also disturbed in dysplasias but does not discriminate between groups. [18][19][20][21] All of these markers have the potential to facilitate the recognition of abnormalities associated with epithelial hyperplasias and dysplasias, but none has yet been shown to be superior to morphological grading.…”

Section: Table 1 Evaluation Of Diagnostic Guidelinesmentioning

confidence: 99%

‘Risky’ epithelium in the larynx — a practical diagnosis?

Helliwell

1999

Histopathology

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Involucrin in Laryngeal Dysplasia: A Marker for Differentiation

Cited by 17 publications

References 11 publications

Combined loss of expression of involucrin and cytokeratin 13 is associated with poor prognosis in squamous cell carcinoma of mobile tongue

Combined loss of expression of involucrin and cytokeratin 13 is associated with poor prognosis in squamous cell carcinoma of mobile tongue

Identification of distinct layers within the stratified squamous epithelium of the adult human true vocal fold

‘Risky’ epithelium in the larynx — a practical diagnosis?

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