Involvement of 14-3-3 Signaling Protein Binding in the Functional Regulation of the Transcriptional Activator REPRESSION OF SHOOT GROWTH by Gibberellins

Ishida, Sarahmi; Fukazawa, Jutarou; Yuasa, Takashi; Takahashi, Yohsuke

doi:10.1105/tpc.104.024604

Cited by 131 publications

(167 citation statements)

References 47 publications

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“…IgG HC, IgG H chain. Ishida et al, 2004Ishida et al, , 2008, whereas other bZIP proteins, such as AREB1/2 (group A) and HY5 (group H), are limited to the nucleus (Chattopadhyay et al, 1998;Yoshida et al, 2010). Nuclear-cytoplasmic shuttling is probably a mechanism for regulating the functions of these proteins.…”

Section: Discussion Factors Involved In the Regulation Of Vip1 Localimentioning

confidence: 99%

A bZIP Protein, VIP1, Is a Regulator of Osmosensory Signaling in Arabidopsis

Tsugama

Liu²,

Takano³

2012

Plant Physiology

118

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Abscisic acid is a stress-related phytohormone that has roles in dehydration and rehydration. In Arabidopsis (Arabidopsis thaliana), two genes that inactivate abscisic acid, CYP707A1 and CYP707A3, are rapidly up-regulated upon rehydration. The factors that regulate CYP707A1/3 are not well characterized. We expressed a bZIP protein, VIP1, as a green fluorescent protein fusion protein in Arabidopsis and found that the nuclear localization of VIP1 was enhanced within 10 min after rehydration. A yeast one-hybrid assay revealed that the amino-terminal region of VIP1 has transcriptional activation potential. In a transient reporter assay using Arabidopsis protoplasts, VIP1 enhanced the promoter activities of CYP707A1/3. In gel shift and chromatin immunoprecipitation analyses, VIP1 directly bound to DNA fragments of the CYP707A1/3 promoters. Transgenic plants expressing VIP1-green fluorescent protein were found to overexpress CYP707A1/3 mRNAs. The time course of nuclearcytoplasmic shuttling of VIP1 was consistent with the time courses of the expression of CYP707A1/3. These results suggest that VIP1 functions as a regulator of osmosensory signaling in Arabidopsis.

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Section: Discussion Factors Involved In the Regulation Of Vip1 Localimentioning

confidence: 99%

A bZIP Protein, VIP1, Is a Regulator of Osmosensory Signaling in Arabidopsis

Tsugama

Liu²,

Takano³

2012

Plant Physiology

118

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“…In barley, 14-3-3 proteins are induced by abscisic acid and function as transcriptional coactivators by binding to ABI5 and related transcription factors to activate abscisic acid -responsive gene expression (44). In tobacco, 14-3-3 protein binding promotes cytoplasmic retention of RSG, which is a transcriptional activator for gibberellins-biosynthetic genes (23,24). Gibberellins and BR are known to promote similar growth responses such as seed germination, stem elongation, and flowering, whereas abscisic acid is known to antagonize most actions of gibberellins and BR.…”

Section: Conserved Structure and Function Of Bzr1 And Osbzr1 Inmentioning

confidence: 99%

“…14-3-3 proteins can regulate their target proteins by changing their stability, enzymatic activity, or subcellular localization (23,24,32). To test whether binding of 14-3-3 proteins affects the subcellular localization of OsBZR1, we compared the localization pattern of the OsBZR1-GFP fusion protein with that of OsBZR1S156G-GFP.…”

Section: Binding By 14-3-3 Proteins Reduces the Nuclear Localization Ofmentioning

confidence: 99%

“…Studies in metazoans have demonstrated important roles of 14-3-3 proteins as major regulators of primary metabolism and cellular signal transduction (21). In plants, 14-3-3 proteins have been shown to interact with large numbers of proteins, which include metabolic enzymes, signaling proteins, and transcription factors (22)(23)(24)(25). However, their specific role in a signal transduction pathway has yet to be illustrated.…”

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Functions of OsBZR1 and 14-3-3 proteins in brassinosteroid signaling in rice

Bai

Zhang²,

Gampala³

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

386

336

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Brassinosteroids (BR) are essential growth hormones found throughout the plant kingdom. BR bind to the receptor kinase BRI1 on the cell surface to activate a signal transduction pathway that regulates nuclear gene expression and plant growth. To understand the downstream BR signaling mechanism in rice, we studied the function of OsBZR1 using reverse genetic approaches and identified OsBZR1-interacting proteins. Suppressing OsBZR1 expression by RNAi resulted in dwarfism, erect leaves, reduced BR sensitivity, and altered BR-responsive gene expression in transgenic rice plants, demonstrating an essential role of OsBZR1 in BR responses in rice. Moreover, a yeast two-hybrid screen identified 14-3-3 proteins as OsBZR1-interacting proteins. Mutation of a putative 14-3-3-binding site of OsBZR1 abolished its interaction with the 14-3-3 proteins in yeast and in vivo. Such mutant OsBZR1 proteins suppressed the phenotypes of the Arabidopsis bri1-5 mutant and showed an increased nuclear distribution compared with the wild-type protein, suggesting that 14-3-3 proteins directly inhibit OsBZR1 function at least in part by reducing its nuclear localization. These results demonstrate a conserved function of OsBZR1 and an important role of 14-3-3 proteins in brassinosteroid signal transduction in rice.steroid ͉ nuclear localization ͉ GSK3 ͉ phosphorylation B rassinosteroids (BR) are polyhydroxylated steroid phytohormones with important roles in regulating a wide range of cellular and development processes, such as stem elongation, senescence, seed germination, reproductive development, and photomorphogenesis (1, 2). BR-deficient and BR-insensitive mutants have been identified in Arabidopsis thaliana (1), pea, tomato, barley, and rice (3). Although strong BR mutants show multiple growth defects, including dwarfism, male sterility, delayed senescence, and constitutive photomorphogenesis in the dark (1), weak BR mutants in rice and barley have increased grain yield because of erect leaves and reduced plant height (4-6). It is believed that manipulating BR level or sensitivity can improve plant productivity.Molecular genetic studies in A. thaliana have illustrated a BRsignaling pathway that includes two receptor kinases (BRI1 and BAK1) on the cell surface, a soluble glycogen synthase kinase 3-like kinase (BIN2), a phosphatase (BSU1), and two homologous transcription factors (BZR1 and BZR2/BES1) (1, 7). BR directly bind to the extracellular domain of BRI1 and activate its intracellular kinase activity (8). Activation of BRI1 leads to interaction with, and activation of, another receptor kinase, BAK1, which has been proposed to act as BRI1's coreceptor (9, 10). Downstream of the receptor kinases, the BIN2 kinase and BSU1 phosphatase regulate the phosphorylation status of BZR1 and BZR2/BES1 (11-14). BR activate BZR1 and BZR2/BES1 by inducing their dephosphorylation, possibly by inhibiting BIN2 or activating BSU1. Activated BZR1 and BES1 bind to promoters of BR target genes to regulate their expression (15, 16).BZR1 and BZR2/BES1 share 88...

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“…RF2a and RF2b, two close VIP1 homologs in rice (Oryza sativa), also are involved in regulating vascular gene expression and in regulating responses to the Rice tungro virus (Yin et al, 1997;Petruccelli et al, 2001;Dai et al, 2004Dai et al, , 2006Dai et al, , 2008. RSG, a close VIP1 homolog in tobacco (Nicotiana tabacum), regulates the biosynthesis of a phytohormone, GA (Fukazawa et al, 2000(Fukazawa et al, , 2011Igarashi et al, 2001;Ishida et al, 2004Ishida et al, , 2008Ito et al, 2010Ito et al, , 2014.…”

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confidence: 99%

The bZIP protein VIP1 is involved in touch responses in Arabidopsis roots

Tsugama¹,

Liu²,

Takano³

2016

Plant Physiol.

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VIP1 is a bZIP transcription factor in Arabidopsis (Arabidopsis thaliana). VIP1 transiently accumulates in the nucleus when cells are exposed to hypoosmotic conditions, but its physiological relevance is unclear. This is possibly because Arabidopsis has approximately 10 close homologs of VIP1 and they function redundantly. To examine their physiological roles, transgenic plants overexpressing a repression domain-fused form of VIP1 (VIP1-SRDXox plants), in which the gene activation mediated by VIP1 is expected to be repressed, were generated. Because hypoosmotic stress can mimic mechanical stimuli (e.g. touch), the touchinduced root-waving phenotypes and gene expression patterns in those transgenic plants were examined. VIP1-SRDXox plants exhibited more severe root waving and lower expression of putative VIP1 target genes. The expression of the VIP1-green fluorescent protein (GFP) fusion protein partially suppressed the VIP1-SRDX-induced increase in root waving when expressed in the VIP1-SRDXox plants. These results suggest that VIP1 can suppress the touch-induced root waving. The VIP1-SRDX-induced increase in root waving was also suppressed when the synthetic auxin 2,4-dichlorophenoxy acetic acid or the ethylene precursor 1-aminocyclopropane-1-carboxylic acid, which is known to activate auxin biosynthesis, was present in the growth medium. Root cap cells with the auxin marker DR5rev::GFP were more abundant in the VIP1-SRDXox background than in the wild-type background. Auxin is transported via the root cap, and the conditions of outermost root cap layers were abnormal in VIP1-SRDXox plants. These results raise the possibility that VIP1 influences structures of the root cap and thereby regulates the local auxin responses in roots.

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Involvement of 14-3-3 Signaling Protein Binding in the Functional Regulation of the Transcriptional Activator REPRESSION OF SHOOT GROWTH by Gibberellins

Cited by 131 publications

References 47 publications

A bZIP Protein, VIP1, Is a Regulator of Osmosensory Signaling in Arabidopsis

A bZIP Protein, VIP1, Is a Regulator of Osmosensory Signaling in Arabidopsis

Functions of OsBZR1 and 14-3-3 proteins in brassinosteroid signaling in rice

The bZIP protein VIP1 is involved in touch responses in Arabidopsis roots

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