Involvement of 5-HT1A in the anxiolytic-like effect of dichloromethane fraction of Pimenta pseudocaryophyllus

Fajemiroye, James Oluwagbamigbe; Galdino, Pablinny Moreira; Alves, Suzana Ferreira; Paula, Joelma Abadia Marciano de; Paula, José Realino de; Ghedini, Paulo César; Costa, Élson Alves

doi:10.1016/j.jep.2012.03.043

Cited by 18 publications

(30 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…DISCUSSION Antioxidants obtained from natural sources may provide new possibilities for the treatment and prevention of oxidative stress-mediated diseases; therefore plant extracts rich in antioxidant compounds have gained special attention (29). As described above, P. pseudocaryophyllus has been used as medicinal plant in folk medication system and is rich in bioactive compounds responsible for that properties (11,12). In this study, we have found that polyphenols content was approximately seven times higher than total of flavonoids content of PPE, corroborating other studies that show that P. pseudocaryophyllus contains not only flavonoids, but other polyphenolic substances, like tannins Fig.…”

Section: In Vivo Studiessupporting

confidence: 90%

“…Commonly, teas prepared with its leaves are used as tranquilizers, digestive regulators, and for the relief of cold symptoms (11). Despite being the only species of the gender native to Brazil (12), there are still few studies with this plant. The leaves of P. pseudocaryophyllus present high concentrations of polyphenolic compounds such as tannins and flavonoids (11,12), which suggests that it might have the ability to act as an antioxidant.…”

Section: Introductionmentioning

confidence: 99%

“…Despite being the only species of the gender native to Brazil (12), there are still few studies with this plant. The leaves of P. pseudocaryophyllus present high concentrations of polyphenolic compounds such as tannins and flavonoids (11,12), which suggests that it might have the ability to act as an antioxidant. Corroborating, it was reported that the antioxidant activity (AA) could be used to evaluate the functional activity of topical functionalized formulations (13,14) and the release of antioxidant components from these formulations (15,16).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Topical Formulations Containing Pimenta pseudocaryophyllus Extract: In Vitro Antioxidant Activity and In Vivo Efficacy Against UV-B-Induced Oxidative Stress

et al. 2013

View full text Add to dashboard Cite

Abstract. Pimenta pseudocaryophyllus is a Brazilian native plant that presents high concentrations of flavonoids and other polyphenolic compounds. Herein, we evaluated: (1) the chemical properties of P. pseudocaryophyllus ethanolic extract (PPE), (2) the in vitro antioxidant activity (AA) of PPE and of two different topical formulations (F1 and F2) containing PPE, (3) physico-chemical and functional stability, (4) in vitro release of PPE, and (5) in vivo capacity of formulations to prevent UV-B irradiation-induced skin damage. Results show that the polyphenol and flavonoid contents in PPE were 199.33 and 28.32 mg/g, respectively, and HPLC results show the presence of eugenol, tannic acid, and rutin. Evaluation of the in vitro AA of PPE demonstrated a dose-dependent effect and an IC 50 of 4.75 μg/mL in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 3.0 μg/mL in 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. The ferric-reducing antioxidant power (FRAP assay) was 0.046 μmol/L trolox equivalent/ μg/mL of extract. Among the AA, only the capacity to scavenge DPPH radical of PPE was maintained in F1 and F2. In addition, both formulations satisfactorily released the extract. The evaluation of the functional stability of F1 and F2 did not demonstrate loss of activity by storage at room temperature and at 4°C/6 months. In irradiated mice, treatment with F1 and F2 added with PPE significantly increased the capacity to scavenge ABTS radical and the FRAP of skin compared to vehicle-treated mice. In conclusion, the present results suggest that formulations containing PPE may be a topical source of antioxidant compounds to decrease oxidative damages of the skin.

show abstract

Section: In Vivo Studiessupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Topical Formulations Containing Pimenta pseudocaryophyllus Extract: In Vitro Antioxidant Activity and In Vivo Efficacy Against UV-B-Induced Oxidative Stress

et al. 2013

View full text Add to dashboard Cite

show abstract

“…For the purpose of our study, the dose of OA was estimated from previous studies in our laboratory on the organic extract (Fajemiroye et al, 2012). The organic extract that demonstrated anxiolytic-and antidepressant-like activities (Fajemiroye et al, 2012(Fajemiroye et al, , 2013 in animal models was composed of OA (relative composition 8%) among other phytoconstituents. This organic extract was administered orally at an effective dose of 250 mg/kg.…”

Section: Introductionmentioning

confidence: 99%

Plurality of anxiety and depression alteration mechanism by oleanolic acid

et al. 2014

View full text Add to dashboard Cite

Our study sought to evaluate the anxiolytic and antidepressant activities of oleanolic acid as well as the neural mechanisms involved. Animal models such as barbiturate sleep-induction, light-dark box, elevated plus maze, forced swimming test, tail suspension test and open field test were conducted. Male Albino Swiss mice were treated orally with vehicle 10 mL/kg, fluoxetine 20 mg/kg, imipramine 15 mg/kg, diazepam 1 mg/kg or oleanolic acid 5-40 mg/kg. Pretreatment (intraperitoneal) of animals with pentylenetetrazole (PTZ) 20 mg/kg, 1-(2-methoxyphenyl)-4-[4- (2-phthalimido) butyl]piperazine hydrobromide (NAN-190) 0.5 mg/kg, p-chlorophenylalanine methyl ester (PCPA) 100 mg/kg or α-methyl-p-tyrosine (AMPT) 100 mg/kg, WAY100635 (WAY) 0.3 mg/kg, prazosin (PRAZ) 1 mg/kg, yohimbine 2 mg/kg as well as monoamine oxidase assay and hippocampal brain-derived neurotrophic factor (BDNF) quantification were carried out. Oleanolic acid potentiated the hypnotic effect of barbiturate and demonstrated an anxiolytic effect in both the light-dark box and elevated plus maze. This effect was not reversed by PTZ. Acute and/or chronic oral treatment of mice with oleanolic acid (5-20 mg/kg) elicited an antidepressant effect in the forced swimming test and the tail suspension test without interfering with the locomotor activity. The antidepressant effect of oleanolic acid was attenuated by NAN-190, AMPT, PCPA, WAY and PRAZ. Although monoamine oxidase activity remained unaltered by oleanolic acid, chronic administration of oleanolic acid augmented hippocampal BDNF level. These findings demonstrate multiple mechanisms of the anxiolytic and antidepressant effect of oleanolic acid.

show abstract

“…Evaluation of the antidepressive-like property of dichloromethane fraction (DF) of Pimenta pseudocaryophyllus is borne out of its anxiolytic property that has been associated with monoaminergic receptor [8]. …”

Section: Introductionmentioning

confidence: 99%

Antidepressive-Like Property of Dichloromethane Fraction ofPimenta pseudocaryophyllusand Relevance of Monoamine Metabolic Enzymes

Fajemiroye

Martins

Ghedini

et al. 2013

Evidence-Based Complementary and Alternative Medicine

Self Cite

View full text Add to dashboard Cite

Pimenta pseudocaryophyllus popularly referred to as craveiro is considered as a calming agent in different local preparations. The present study attempted to examine antidepressant-like effect of dichloromethane fraction (DF) and role of monoamine oxidase (MAO), tryptophan, and tyrosine hydroxylase. Based on the research focus, tail suspension (TS), forced swimming (FS), and open field (OF) tests were conducted after oral administration of DF (125, 250, or 500 mg/Kg). Ex vivo assay of MAO was also conducted to evaluate inhibitory effect of DF (250 mg/Kg). Administration of DF elicits antidepressant-like response in the TS and FS. However, DF 500 mg/Kg did not alter mice performance in these models. The data obtained in the OF showed a reduction in total crossing and rearing activity; these effects suggest motor interference in TS and FS performance. Mice pretreatment with p-chlorophenylalanine methyl ester (PCPA) (100 mg/kg, i.p.—serotonin biosynthesis inhibitor) for 4 consecutive days or acute administration of α-methyl-p-tyrosine (AMPT) (100 mg/kg, i.p.—catecholamine synthesis inhibitor) blocked anti-immobility effect of DF in the FS. In ex vivo assay of MAO, DF did not inhibit catabolic activity of MAO. Our findings support antidepressant-like activity of DF and suggest an effect that depends on monoamine biosynthesis.

show abstract

Involvement of 5-HT1A in the anxiolytic-like effect of dichloromethane fraction of Pimenta pseudocaryophyllus

Cited by 18 publications

References 31 publications

Topical Formulations Containing Pimenta pseudocaryophyllus Extract: In Vitro Antioxidant Activity and In Vivo Efficacy Against UV-B-Induced Oxidative Stress

Topical Formulations Containing Pimenta pseudocaryophyllus Extract: In Vitro Antioxidant Activity and In Vivo Efficacy Against UV-B-Induced Oxidative Stress

Plurality of anxiety and depression alteration mechanism by oleanolic acid

Antidepressive-Like Property of Dichloromethane Fraction ofPimenta pseudocaryophyllusand Relevance of Monoamine Metabolic Enzymes

Contact Info

Product

Resources

About