Involvement of alternative oxidase (AOX) in adventitious rooting of Olea europaea L. microshoots is linked to adaptive phenylpropanoid and lignin metabolism

Macedo, Elisete Santos; Sircar, Debabrata; Cardoso, Hélia; Peixe, Augusto; Arnholdt‐Schmitt, Birgit

doi:10.1007/s00299-012-1272-6

Cited by 39 publications

(34 citation statements)

References 37 publications

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“…Further evidence showed that H 2 O 2 treatments, which enhanced adventitious rooting, stimulated PPO activity in Chrysanthemum (Liao et al, 2010), possibly through activation of AOX (Santos Macedo et al, 2009). IBA treatments promote AOX gene transcription in olive (Santos Macedo et al, 2012), which in turn can stimulate phenylpropanoid biosynthesis (Sircar et al, 2012;Vogt, 2010) leading to an increased concentration of monophenolic compounds, which are natural substrates of PPO. This would also explain why in microshoots treated with SHAM, an AOX inhibitor, no visible changes in PPO were detected.…”

Section: Temporal Changes In Oxidative Enzymes Activitymentioning

confidence: 98%

“…The base of each explant (approx. 1.0 cm) was submitted to a 10 s quick-dip treatment either in a sterile solution of 14.7 mM IBA or a sterile solution of 14.7 mM IBA plus 100 mM SHAM (concentration optimized by Santos Macedo et al, 2012). The explants were then inoculated, in vitro, in 500 ml glass flasks containing 75 ml semi-solid olive culture medium (OM), devoid of plant growth regulators and supplemented with 7 g L -1 commercial agar-agar, 30 g L -1 D-mannitol and 2 g L -1 activated charcoal (Rugini, 1984).…”

Section: Plant Material Rooting Procedures and Culture Conditionsmentioning

confidence: 99%

“…'Cobrançosa' under in vitro culture conditions, microshoots of the cultivar 'Galega Vulgar' were treated with salicylhydroxamic acid (SHAM). SHAM has been shown to inhibit root formation in olive (Santos Macedo et al, 2012), thus providing a negative control by imitating a difficult-to-root cultivar.…”

Section: Introductionmentioning

confidence: 99%

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Tracking biochemical changes during adventitious root formation in olive (Olea europaea L.)

Porfírio

Calado

Noceda

et al. 2016

Scientia Horticulturae

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The activity of oxidative enzymes and the levels of free auxins were determined during adventitious root formation in olive explants. Rooting trials were performed both with in vitro-cultured microshoots of the cultivar 'Galega Vulgar', treated with indole-3-butyric acid (IBA) and with salicylhydroxamic acid (SHAM) + IBA, as well as with semi-hardwood cuttings of the cultivars 'Galega Vulgar' (difficult-to-root) and 'Cobrançosa' (easy-to-root), treated with IBA. The auxin (IBA) was used in all experiments as a rooting promoter, while SHAM was used in micropropagation trials as rooting inhibitor, providing a negative control. Free indole-3-acetic acid (IAA) and IBA concentrations were determined in microshoots, as well as in semi-hardwood cuttings, throughout the rooting period at pre-established time-points. At the same time-points, the enzymatic activity of polyphenol oxidases (PPO), peroxidases (POX), and IAA oxidase (IAAox) was evaluated in the microshoots. Microshoots treated with SHAM+IBA revealed higher POX and IAAox activity, as well as lower PPO activity, than those treated only with IBA. IAA levels were higher in IBA-treated microshoots during induction phase, but lower during early initiation phase. In contrast, free IBA levels were higher in microshoots treated with SHAM+IBA during induction, but lower during initiation. A similar pattern of free auxin levels was observed in semi-hardwood cuttings of the two contrasting cultivars under evaluation. The similarities found on the auxin patterns of microshoots treated with SHAM and those of semi-hardwood cuttings of the difficult-to-root olive cultivar allow considering SHAM a reliable control for when simulation of a difficult-to-root behavior is necessary. The inhibitory effect of SHAM in root formation could be related with 1) the inhibition of alternative oxidase (AOX), leading to a downregulation of phenylpropanoid biosynthetic pathways, which would decrease the concentration of phenolic substrates for PPO; 2) an increase in IAAox activity resulting in lower free IAA levels or; 3) a defective conversion of IBA into IAA.

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Section: Temporal Changes In Oxidative Enzymes Activitymentioning

confidence: 98%

Section: Plant Material Rooting Procedures and Culture Conditionsmentioning

confidence: 99%

See 1 more Smart Citation

Tracking biochemical changes during adventitious root formation in olive (Olea europaea L.)

Porfírio

Calado

Noceda

et al. 2016

Scientia Horticulturae

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“…Acclimation to stress is often accompanied by metabolic re-adjustment. The alternative oxidase (AOX) plays a central role in determining reactive oxygen species equilibrium in plants and can be induced in response to diverse abiotic and biotic stress conditions (Santos-Macedo et al, 2012). Secondary metabolism during AR may be associated with AOX activity.…”

Section: Wound Responsementioning

confidence: 99%

“…Moreover, a complex interaction between AOX and H 2 O 2 signaling is apparent. Application of H 2 O 2 could replace added auxin as a rooting agent in olive cuttings (Santos-Macedo et al, 2009) and the presence of an AOX inhibitor, salicylhydroxamic acid (SHAM), reduced rooting even in presence of exogenous auxin (Santos-Macedo et al, 2012). …”

Section: Wound Responsementioning

confidence: 99%

When stress and development go hand in hand: main hormonal controls of adventitious rooting in cuttings

Costa¹,

Almeida²,

Ruedell³

et al. 2013

Front. Plant Sci.

311

280

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Adventitious rooting (AR) is a multifactorial response leading to new roots at the base of stem cuttings, and the establishment of a complete and autonomous plant. AR has two main phases: (a) induction, with a requirement for higher auxin concentration; (b) formation, inhibited by high auxin and in which anatomical changes take place. The first stages of this process in severed organs necessarily include wounding and water stress responses which may trigger hormonal changes that contribute to reprogram target cells that are competent to respond to rooting stimuli. At severance, the roles of jasmonate and abscisic acid are critical for wound response and perhaps sink strength establishment, although their negative roles on the cell cycle may inhibit root induction. Strigolactones may also inhibit AR. A reduced concentration of cytokinins in cuttings results from the separation of the root system, whose tips are a relevant source of these root induction inhibitors. The combined increased accumulation of basipetally transported auxins from the shoot apex at the cutting base is often sufficient for AR in easy-to-root species. The role of peroxidases and phenolic compounds in auxin catabolism may be critical at these early stages right after wounding. The events leading to AR strongly depend on mother plant nutritional status, both in terms of minerals and carbohydrates, as well as on sink establishment at cutting bases. Auxins play a central role in AR. Auxin transporters control auxin canalization to target cells. There, auxins act primarily through selective proteolysis and cell wall loosening, via their receptor proteins TIR1 (transport inhibitor response 1) and ABP1 (Auxin-Binding Protein 1). A complex microRNA circuitry is involved in the control of auxin response factors essential for gene expression in AR. After root establishment, new hormonal controls take place, with auxins being required at lower concentrations for root meristem maintenance and cytokinins needed for root tissue differentiation.

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Technical protocol for mitochondria isolation for different studies

Horn

2015

Alternative Respiratory Pathways in Higher Plants

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Involvement of alternative oxidase (AOX) in adventitious rooting of Olea europaea L. microshoots is linked to adaptive phenylpropanoid and lignin metabolism

Abstract: The manuscript reports the general importance of AOX in olive adventitious rooting and the association of alternative respiration to adaptive phenylpropanoid and lignin metabolism.

Cited by 39 publications

References 37 publications

Tracking biochemical changes during adventitious root formation in olive (Olea europaea L.)

Tracking biochemical changes during adventitious root formation in olive (Olea europaea L.)

When stress and development go hand in hand: main hormonal controls of adventitious rooting in cuttings

Technical protocol for mitochondria isolation for different studies

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