Involvement of an Oct4-related PouV gene, pou5f3/pou2, in neurogenesis in the early neural plate of zebrafish embryos

Inomata, Chihiro; Yuikawa, Tatsuya; Nakayama-Sadakiyo, Yukiko; Kobayashi, Kana; Ikeda, Masa‐Aki; Chiba, Masanori; Konishi, Chihiro; Ishioka, Akiko; Tsuda, Sachiko; Yamasu, Kyo

doi:10.1016/j.ydbio.2019.09.002

Cited by 8 publications

(13 citation statements)

References 94 publications

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“…We first focused on the zebrafish paralogues of mammalian genes involved in pluripotency (i.e., pou5f3 , sox2 , klf17 , nanog , myc , and sall4 ). Expression of pou5f3 was observed on the dorsal side of the tail bud in addition to the previously described anterior midbrain and hindbrain (Inomata et al., 2020; Figure 1A). sox2 was not expressed in the tail bud region (Figure Aa), whereas two other zebrafish soxB1 genes, sox3 and sox19a , were expressed weakly (Figure 1B) and strongly (Figure 1C), respectively, in the dorsal tail bud (similarly to pou5f3 ).…”

Section: Resultssupporting

confidence: 75%

“…This region was further shown to express neurogenesis genes such as two soxB1 genes ( sox3 and sox19a ), neurog1 , deltaA , and her4.1/her9 , implicating pou5f3 in neural specification in this region. Indeed, we recently found that pou5f3 is involved in neural development in the hindbrain (Inomata et al., 2020). Broad expression of notch1a throughout the tail bud indicates that this region is responsive to Delta‐mediated lateral inhibition.…”

Section: Discussionmentioning

confidence: 99%

“…Intense sox19a expression throughout the neural tube suggests its involvement in the entire neurogenesis process. In this regard, despite the similar functions of soxB1 genes (Okuda et al., 2010), respective soxB1 genes are reportedly regulated in distinct manners (Inomata et al., 2020; Rogers et al., 2008) and have different roles in embryos (Rogers et al., 2009). We previously observed differences in the regulatory functions of Sox2 and Sox3 in vitro in the presence of Pou5f3 (Kobayashi et al., 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Of note, endogenous expression of pou5f3 was enhanced in the caudal region, suggesting negative feedback regulation of pou5f3 expression. We previously showed that pou5f3 is involved in neurogenesis in the hindbrain (Inomata et al., 2020). Furthermore, all three soxB1 genes examined as well as sall4 , which are known to play pivotal roles in early neurogenesis, were downregulated in the posterior neural plate/tube.…”

Section: Discussionmentioning

confidence: 99%

“…In zebrafish, pou5f3 is expressed maternally throughout embryos, but this expression is progressively restricted to the neural plate and then to the tail bud during gastrulation (Hauptmann & Gerster, 1995; Takeda et al., 1994; Figure ). Of note, the expression pattern almost coincides with proneural clusters in the forebrain, midbrain, hindbrain, and spinal cord at early somite stages, suggesting a role in primary neurogenesis (Inomata et al., 2020). Importantly, after early somitogenesis, pou5f3 expression is observed specifically in the posterior end of the embryo (Figure ).…”

Section: Introductionmentioning

confidence: 96%

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Involvement of Oct4‐type transcription factor Pou5f3 in posterior spinal cord formation in zebrafish embryos

et al. 2021

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In vertebrate embryogenesis, elongation of the posterior body is driven by de novo production of the axial and paraxial mesoderm as well as the neural tube at the posterior end. This process is presumed to depend on the stem cell‐like population in the tail bud region, but the details of the gene regulatory network involved are unknown. Previous studies suggested the involvement of pou5f3, an Oct4‐type POU gene in zebrafish, in axial elongation. In the present study, we first found that pou5f3 is expressed mainly in the dorsal region of the tail bud immediately after gastrulation, and that this expression is restricted to the posterior‐most region of the elongating neural tube during somitogenesis. This pou5f3 expression was complementary to the broad expression of sox3 in the neural tube, and formed a sharp boundary with specific expression of tbxta (orthologue of mammalian T/Brachyury) in the tail bud, implicating pou5f3 in the specification of tail bud‐derived cells toward neural differentiation in the spinal cord. When pou5f3 was functionally impaired after gastrulation by induction of a dominant‐interfering pou5f3 mutant gene (en‐pou5f3), trunk and tail elongation were markedly disturbed at distinct positions along the axis depending on the stage. This finding showed involvement of pou5f3 in de novo generation of the body from the tail bud. Conditional functional abrogation also showed that pou5f3 downregulates mesoderm‐forming genes but promotes neural development by activating neurogenesis genes around the tail bud. These results suggest that pou5f3 is involved in formation of the posterior spinal cord.

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Section: Resultssupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

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Involvement of Oct4‐type transcription factor Pou5f3 in posterior spinal cord formation in zebrafish embryos

et al. 2021

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Elongation of the developing spinal cord is driven by Oct4‐type transcription factor‐mediated regulation of retinoic acid signaling in zebrafish embryos

Yuikawa,

Sato,

Ikeda

et al. 2023

Developmental Dynamics

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BackgroundElongation of the spinal cord is dependent on neural development from neuromesodermal progenitors in the tail bud. We previously showed the involvement of the Oct4‐type gene, pou5f3, in this process in zebrafish mainly by dominant‐interference gene induction, but, to compensate for the limitation of this transgene approach, mutant analysis was indispensable. pou5f3 involvement in the signaling pathways was another unsolved question.ResultsWe examined the phenotypes of pou5f3 mutants and the effects of Pou5f3 activation by the tamoxifen‐ERT2 system in the posterior neural tube, together confirming the involvement of pou5f3. The reporter assays using P19 cells implicated tail bud‐related transcription factors in pou5f3 expression. Regulation of tail bud development by retinoic acid (RA) signaling was confirmed by treatment of embryos with RA and the synthesis inhibitor, and in vitro reporter assays further showed that RA signaling regulated pou5f3 expression. Importantly, the expression of the RA degradation enzyme gene, cyp26a1, was down‐regulated in embryos with disrupted pou5f3 activity.ConclusionsThe involvement of pou5f3 in spinal cord extension was supported by using mutants and the gain‐of‐function approach. Our findings further suggest that pou5f3 regulates the RA level, contributing to neurogenesis in the posterior neural tube.

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Spatiotemporal expression patterns of maternal-zygotic gene pou5f3 in hybrid fish embryos

Wang,

Zhang,

Wang

et al. 2025

Aquaculture

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Involvement of an Oct4-related PouV gene, pou5f3/pou2, in neurogenesis in the early neural plate of zebrafish embryos

Cited by 8 publications

References 94 publications

Involvement of Oct4‐type transcription factor Pou5f3 in posterior spinal cord formation in zebrafish embryos

Involvement of Oct4‐type transcription factor Pou5f3 in posterior spinal cord formation in zebrafish embryos

Elongation of the developing spinal cord is driven by Oct4‐type transcription factor‐mediated regulation of retinoic acid signaling in zebrafish embryos

Spatiotemporal expression patterns of maternal-zygotic gene pou5f3 in hybrid fish embryos

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