Involvement of apolipoprotein D in desiccation tolerance and adult fecundity of Acyrthosiphon pisum

Qiao, Jian-Wen; Fan, Yongliang; Wu, Bingjin; Wang, Dun; Liu, Tong‐Xian

doi:10.1016/j.jinsphys.2020.104160

Cited by 6 publications

(7 citation statements)

References 52 publications

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“…As for the duration of RNAi suppression, the RNAi efficiency for downregulating ApCPR reached the maximum on the 2nd day after dsRNA injection, and the effective silencing lasted until the 5th day. This result was similar to that of a previous study on ApApoD and cathepsin‐l of A. pisum which describes a significant silencing effect maintained for 5 days after dsRNA injection in the 3rd instar nymphs (Sapountzis et al ., 2014; Qiao et al ., 2020); in contrast, a study on ApLpR and CYP4G51 showed that the inhibition effect lasts for only 4 and 3 days, respectively (Chen et al ., 2016; Qiao et al ., 2021). These studies suggested that the duration of the gene silencing effect in A. pisum may differ depending on the gene and induced short‐term RNAi effects.…”

Section: Discussionmentioning

confidence: 95%

“…The most effective dose targeting ApCPR was found to be 1.82 μg, and RNAi efficiency was slightly higher than that obtained using a dose of 1.52 μg. Our previous studies found that dsRNA doses exceeding the optimal dose for ApLpR and ApApoD do not further increase the RNAi efficiency; in contrast, the interference efficiency of ApLpR has been shown to decrease (Qiao et al ., 2020, 2021). This may be attributed to a relatively higher activity of RNase induced by excessive dsRNA doses (Niu et al ., 2019).…”

Section: Discussionmentioning

confidence: 99%

“…To analyze the expression level of ApCPR in response to desiccation, the 3rd instar aphids (n = ≈50) were placed in a 5% RH drying tube (50 mL); the specific preparation method was described in our previous study (Qiao et al, 2020(Qiao et al, , 2021). An RH of 70% was used as a control.…”

Section: Evaluation Of Desiccation Tolerancementioning

confidence: 99%

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Downregulation of NADPH‐cytochrome P450 reductase via RNA interference increases the susceptibility of Acyrthosiphon pisum to desiccation and insecticides

Qiao

Fan

et al. 2022

Insect Science

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Nicotinamide adenine dinucleotide phosphate (NADPH)‐cytochrome P450 reductase (CPR) is involved in the metabolism of endogenous and exogenous substances, and detoxification of insecticides. RNA interference (RNAi) of CPR in certain insects causes developmental defects and enhanced susceptibility to insecticides. However, the CPR of Acyrthosiphon pisum has not been characterized, and its function is still not understood. In this study, we investigated the biochemical functions of A. pisum CPR (ApCPR). ApCPR was found to be transcribed in all developmental stages and was abundant in the embryo stage, and in the gut, head, and abdominal cuticle. After optimizing the dose and silencing duration of RNAi for downregulating ApCPR, we found that ApCPR suppression resulted in a significant decrease in the production of cuticular and internal hydrocarbon contents, and of cuticular waxy coatings. Deficiency in cuticular hydrocarbons (CHCs) decreased the survival rate of A. pisum under desiccation stress and increased its susceptibility to contact insecticides. Moreover, desiccation stress induced a significant increase in ApCPR mRNA levels. We further confirmed that ApCPR participates in CHC production. These results indicate that ApCPR modulates CHC production, desiccation tolerance, and insecticide susceptibility in A. pisum, and presents a novel target for pest control.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

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Downregulation of NADPH‐cytochrome P450 reductase via RNA interference increases the susceptibility of Acyrthosiphon pisum to desiccation and insecticides

Qiao

Fan

et al. 2022

Insect Science

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“…Apolipoprotein D is a lipocalin superfamily member that plays important roles in lipid metabolism, cell differentiation, aging, stress resistance, ootheca formation, and reproduction [ 39 , 40 , 41 , 42 ]. Takeout protein has the characteristics of small soluble proteins and is most similar to juvenile hormone binding protein [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

Proteinaceous Venom Expression of the Yellow Meadow Ant, Lasius flavus (Hymenoptera: Formicidae)

Wang

Xiao

et al. 2023

Toxins

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Ants are one of the important groups of venomous animals with about 14,000 described species. Studies so far focused on the discovery of venom proteins are only available for limited stinging ants, and the proteinaceous compositions of the stingless ants are completely unknown. Here, we used the transcriptomic approach to identify venom components from the yellow meadow ant, Lasius flavus, a stingless ant. The transcriptomic analysis yielded an extraordinary simplicity of the venom expression profile, with 17 venom proteins, such as phospholipase B, odorant binding protein, and apolipoprotein D. Ten of them were discovered as novel toxins for future functional investigations. Quantitative real time PCR analysis revealed that genes encoding the identified venom proteins display exclusively or highly expression profiles in venom glands, validating them as venom compositions. Our findings contribute to the understanding of the evolutional diversity of toxins between stinging and stingless ants.

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“…Insects undergo many environmental stresses in natural environments and have evolved a wide range of mechanisms to cope with them. Accumulating evidence indicates that lipocalin genes are involved in the response to abiotic stresses (Zhou et al, 2018(Zhou et al, , 2020Qiao et al, 2020). Lipocalins have been implicated in the regulation of cell homoeostasis and the modulation of the stress response, and, as transport proteins, to act in the general clearance of endogenous and exogenous compounds (Flower, 1996).…”

Section: Discussionmentioning

confidence: 99%

Genome‐wide identification and comparative analysis of lipocalin families in Lepidoptera with an emphasis on Bombyx mori

et al. 2022

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Lipocalins exhibit functional diversity, including roles in retinol transport, invertebrate cryptic coloration, and stress response. However, genome-wide identification and characterization of lipocalin in the insect lineage have not been thoroughly explored. Here, we found that a lineage-specific expansion of the lipocalin genes in Lepidoptera occurred in large part due to tandem duplication events and several lipocalin genes involving insect coloration were expanded more via tandem duplication in butterflies. A comparative analysis of conserved motifs showed both conservation and divergence of lepidopteran lipocalin family protein structures during evolution. We observe dynamic changes in tissue expression preference of paralogs in Bombyx mori, suggesting differential contribution of paralogs to specific organ functions during evolution. Subcellular localization experiments revealed that lipocalins localize to the cytoplasm, nuclear membrane, or nucleus in BmN cells. Moreover, several lipocalin genes exhibited divergent responses to abiotic and biotic stresses, and 1 lipocalin gene was upregulated by 300 fold in B. mori. These results suggest that lipocalins act as signaling components in defense responses by mediating crosstalk between abiotic and biotic stress responses. This study deepens our understanding of the comprehensive characteristics of lipocalins in insects.

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Involvement of apolipoprotein D in desiccation tolerance and adult fecundity of Acyrthosiphon pisum

Cited by 6 publications

References 52 publications

Downregulation of NADPH‐cytochrome P450 reductase via RNA interference increases the susceptibility of Acyrthosiphon pisum to desiccation and insecticides

Downregulation of NADPH‐cytochrome P450 reductase via RNA interference increases the susceptibility of Acyrthosiphon pisum to desiccation and insecticides

Proteinaceous Venom Expression of the Yellow Meadow Ant, Lasius flavus (Hymenoptera: Formicidae)

Genome‐wide identification and comparative analysis of lipocalin families in Lepidoptera with an emphasis on Bombyx mori

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