Involvement of arachidonic acid metabolites in increases in vascular permeability in experimental dental pulpal inflammation in the rat

Gene Expression Analysis of Membrane Transport Proteins in Normal and Lipopolysaccharide-inflamed Rat Dental Pulp

“…Moreover, increases in mRNA might not always be related to gain of function; global gain or loss of the function of the transporters on the plasma membrane of cells might be regulated by several mechanisms such as post-transcriptional modifications, trafficking, internalization, and degradation. However, the overall level of intercellular PGs might be elevated, because LPS administration is known to induce PGE 2 -mediated inflammatory changes such as the increase in vascular permeability in the rat dental pulp (12).…”

“…Experimental pulp inflammation was induced by applying bacterial LPS (Escherichia coli 0.55:B5), according to the method previously described (11,12). Under pentobarbital sodium anesthesia (30 mg/kg, intraperitoneally), the crowns of upper incisors were cut off at the gingival margin, and the coronal pulp chamber was instrumented to size 45 and to 7 mm in length with stainless steel files.…”

“…Regarding the dental pulp, PGE 2 is considered an important modulator of inflammation, because PGE 2 synthesis increases in experimentally inflamed pulps of rats (11)(12)(13) and dogs (14), and PGE 2 levels in pulp blood samples are higher in inflamed pulps than in clinically intact teeth (15). NSAIDs reduce the increase of PGE 2 synthesis and vascular permeability in lipopolysaccharide (LPS)-stimulated rat incisor pulps (12), which might implicate PGE 2 in the increase of vascular permeability.…”

“…NSAIDs reduce the increase of PGE 2 synthesis and vascular permeability in lipopolysaccharide (LPS)-stimulated rat incisor pulps (12), which might implicate PGE 2 in the increase of vascular permeability. Moreover, PGE 2 has also been implicated in pulpal pain on the basis of the findings that systemic NSAIDs cause inhibition of stimulated intradental nerve activity in cats (16), and that PGE 2 levels are higher in painful pulps than in clinically asymptomatic pulps (17).…”

IL-1&beta; production and gene expression by peptidoglycan from <i>Lactobacillus casei</i> in human dental pulp cells of deciduous teeth and permanent teeth

“…PGE2has been strongly implicated in pulpal inflammation, particularly vascular permeability. (8) Hence, the author thought that peptidoglycan from L. casei in microorganisms of carious lesions would induce inflammation reaction in HPF most strongly.…”