Involvement of Carbonic Anhydrase CAH3 in the Structural and Functional Stabilization of the Water-Oxidizing Complex of Photosystem II from Chlamydomonas reinhardtii

Shukshina, A.K.; Terentyev, Vasily V.

doi:10.1134/s0006297921070075

Cited by 4 publications

(4 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is supported by the increased portion of the low-potential form of the cytochrome b559 in PSII from the cia3 mutant [26]. Later, it was shown that the WOC in PSII from the cia3 mutant is indeed characterized by lower functional stability under increased Cl − content with a simultaneously stronger binding of the PsbP protein to the WOC [27]. Interestingly, the inhibition of CA activity had no influence on the obtained results, directly indicating the structural role of the CAH3 protein in the PSII complex of C. reinhardtii.…”

Section: Functional and Structural Interconnection Between Cah3 And Psiimentioning

confidence: 91%

“…Thus, the absence of CAH3 in the lumen of tubules can suppress the supply of CO 2 for fixation by RuBisCO impairing the CCM. It should be noted that the authors of [58] used relatively distinct strains (CC-400, cia3) compared, for example, with the works by [10,23,[25][26][27], where closely related strains (CC-503, cia3) obtained from WT CC-137c [59] were used. Cells were not preadapted to low-CO 2 conditions, and measurements required a long assay period to complete each data set.…”

Section: Involvement Cah3 In Ccmmentioning

confidence: 99%

“…Interestingly, to date, CAH3 from C. reinhardtii has been the only CA clearly observed in PSII preparations [10,23,[25][26][27] isolated from cyanobacteria, algae, and higher plants whose activity supports the function of the WOC. This makes CAH3 a unique protein for the study of the involvement of CA(s) in the functioning of the photosynthetic apparatus in general and of PSII in particular.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

CAH3 from Chlamydomonas reinhardtii: Unique Carbonic Anhydrase of the Thylakoid Lumen

Terentyev,

Shukshina

2024

Cells

View full text Add to dashboard Cite

CAH3 is the only carbonic anhydrase (CA) present in the thylakoid lumen of the green algae Chlamydomonas reinhardtii. The monomer of the enzyme has a molecular weight of ~29.5 kDa with high CA activity. Through its dehydration activity, CAH3 can be involved either in the carbon-concentrating mechanism supplying CO2 for RuBisCO in the pyrenoid or in supporting the maximal photosynthetic activity of photosystem II (PSII) by accelerating the removal of protons from the active center of the water-oxidizing complex. Both proposed roles are considered in this review, together with a description of the enzymatic parameters of native and recombinant CAH3, the crystal structure of the protein, and the possible use of lumenal CA as a tool for increasing biomass production in higher plants. The identified involvement of lumenal CAH3 in the function of PSII is still unique among green algae and higher plants and can be used to understand the mechanism(s) of the functional interconnection between PSII and the proposed CA(s) of the thylakoid lumen in other organisms.

show abstract

Section: Functional and Structural Interconnection Between Cah3 And Psiimentioning

confidence: 91%

Section: Involvement Cah3 In Ccmmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

CAH3 from Chlamydomonas reinhardtii: Unique Carbonic Anhydrase of the Thylakoid Lumen

Terentyev,

Shukshina

2024

Cells

View full text Add to dashboard Cite

show abstract

“…The two strains of C. reinhardtii studied in the present work were the cell wall-less mutant CC-503 cw92 mt+, taken as the standard wild type (WT) in the series of previous work [ 3 , 22 , 30 , 31 , 32 ], and the basic 137c (CC-125 mt+) WT. Cultures were grown photoautotrophically in parallel in the minimal medium at 25 °C under aeration with air enriched with 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

Action of 2,6-Dichloro-1,4-benzoquinone on the O2-Evolving Activity of Photosystem II in Chlamydomonas reinhardtii Cells with and without Cell Wall: Inhibitory Effect of Its Oxidized Form

Terentyev

Shukshina

Chetverkina

2023

Cells

View full text Add to dashboard Cite

Chlamydomonas reinhardtii is a widely used object in studies on green algae concerning both photosynthesis aspects and possible biotechnological approaches. The measurement of the maximum O2 evolution by photosystem II (PSII) in living algal cells in the presence of artificial acceptors is one of the commonly used methods for determining the photosynthetic apparatus state or its change as compared to a control, parent strain, etc., because PSII is the most sensitive component of the thylakoid membrane. The present study shows the need to use low concentrations of 2,6-dichloro-1,4-benzoquinone (DCBQ) paired with potassium ferricyanide (FeCy) for achieving the maximum O2 evolution rate, while a DCBQ concentration above certain threshold results in strong suppression of O2 evolution. The required DCBQ concentration depends on the presence of the cell wall and should be exactly ~0.1 mM or in the range of 0.2–0.4 mM for cells with and without a cell wall, respectively. The inhibition effect is caused, probably, by a higher content of DCBQ in the oxidized form inside cells; this depends on the presence of the cell wall, which influences the efficiency of DCBQ diffusion into and out of the cell, where it is maintained by FeCy in the oxidized state. The possible mechanism of DCBQ inhibition action is discussed.

show abstract

Macromolecular conformational changes in photosystem II: interaction between structure and function

Terentyev

2022

Biophys Rev

View full text Add to dashboard Cite

Involvement of Carbonic Anhydrase CAH3 in the Structural and Functional Stabilization of the Water-Oxidizing Complex of Photosystem II from Chlamydomonas reinhardtii

Cited by 4 publications

References 50 publications

CAH3 from Chlamydomonas reinhardtii: Unique Carbonic Anhydrase of the Thylakoid Lumen

CAH3 from Chlamydomonas reinhardtii: Unique Carbonic Anhydrase of the Thylakoid Lumen

Action of 2,6-Dichloro-1,4-benzoquinone on the O2-Evolving Activity of Photosystem II in Chlamydomonas reinhardtii Cells with and without Cell Wall: Inhibitory Effect of Its Oxidized Form

Macromolecular conformational changes in photosystem II: interaction between structure and function

Contact Info

Product

Resources

About