Involvement of cell oxidant status and redox state in the increased non-enzymatic ethanol oxidation by the regenerating rat liver

Hernández‐Muñoz, Rolando; Contreras-Zentella, Martha Lucinda

doi:10.1016/j.bcp.2019.01.003

Cited by 5 publications

(4 citation statements)

References 52 publications

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“…Calculations and Statistics. Cytoplasmic and mitochondrial NAD/NADH ratios were calculated as follows: NAD/NADH = [oxidized substrate]/[reduced substrate] • 1/Keq, taking into account equilibrium constants for lactate, and β-hydroxybutyrate dehydrogenases (28). Linear regression analyses were made using the equation from Pearson's approach.…”

Section: Methodsmentioning

confidence: 99%

The Role of Insulin/glucagon Ratio and Cell Redox State in the Hyperglycemia Induced by Exposure to Pulsed Electromagnetic Fields in Rats

Martiñón-Gutiérrez

Luna-Castro

Hernández‐Muñoz

2020

Preprint

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Background: The exposure to extremely low-frequency electromagnetic fields (EMFs) with a might adversely affect the endocrine system and reproductive functions, as well as the development of animals. Nonetheless, the use of EMFs in the form of magneto-therapy exerts beneficial actions in pain amelioration, post-stroke recovery, or in bone fractures, where EMFs can affect glucose and lipids metabolism, inducing hyperglycemia and a “diabetic” response. Methods: Therefore, we used fed or fasted male rats for a single exposure to 60-Hz EMFs, determining serum levels of glucose, lipids, and indicators of cellular redox state and energy parameter, as well as to perform a metabolic tracing of glucose in the whole animal. Results: A single exposure to EMFs induced hyperglycemia in both, fed and fasted rats, accompanied by an attenuation of a second serum insulin peak. In addition, EMFs also decreased serum levels of free fatty acids and lactate, while they increased those of pyruvate and acetoacetate. Changes in blood glucose level were related with a more oxidized cellular redox state and with the insulin/glucagon ratio. Moreover, rat’s glucose metabolism also coincided with variations in insulin and glucagon secretion, probably depending on cell redox state; these EMFs effects were not modified in animals previously subjected to a chronic exposure (14 days). Conclusions: Increased serum glucose levels and glucose metabolism induced by EMFs exposure were closely related with the cellular redox state and with the insulin/glucagon ratio, whereas changes in the metabolic energy availability seemed to play a minor role in these EMFs-induced metabolic modifications.

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Section: Methodsmentioning

confidence: 99%

The Role of Insulin/glucagon Ratio and Cell Redox State in the Hyperglycemia Induced by Exposure to Pulsed Electromagnetic Fields in Rats

Martiñón-Gutiérrez

Luna-Castro

Hernández‐Muñoz

2020

Preprint

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“…Cytoplasmic and mitochondrial NAD/NADH ratios were calculated using the following formula: NAD/NADH = [oxidized substrate] / [reduced substrate] • 1/Keq, while considering equilibrium constants for lactate and β-hydroxybutyrate dehydrogenases [22]. The results are presented as mean ± SE, and statistical significance of differences was evaluated through a two-way ANOVA assuming normal data distribution.…”

Section: Calculations and Statisticsmentioning

confidence: 99%

Involvement of Liver Redox State in the Zi-Dovudine-Induced Hepatic Steatosis and Its Partial Prevention by Adenosine

Contreras-Zentella,

Butanda-Ochoa,

Sánchez-Sevilla

et al. 2023

Preprint

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Zidovudine (AZT) has significantly reduced the mortality and morbidity rates among AIDS patients, but it has been associated with hepatotoxicity. AZT treatment has been linked to disrupted lipid and glucose metabolism, and the enhancement of pro-inflammatory chemokines and other mediators, which can lead to liver steatosis. In contrast, the administration of adenosine (ADO) has proven to be an effective hepatoprotector against both acute and chronic liver damage. Therefore, present study was aimed to investigate the harmful effects of chronic AZT administration and the beneficial effects of the co-administered ADO. Chronic oral AZT administration did not induce hyperglycemia or dyslipidemia, but serum liver marker enzyme activities were increased, as well as evidence of liver steatosis and inflammation. These findings were associated with low insulin and glucagon levels, elevated serum levels of pro-inflammatory cytokines and disruptions in cell redox states in these animals. ADO co-administration attenuated the deleterious effects induced by AZT: blocking the production of inflammatory molecules, increasing serum insulin and glucagon levels, and restoring liver cellular redox state. Then, blood insulin levels seemed to be greatly influenced by the cellular redox state, which was altered by AZT administration. This alteration is likely to be connected to mitochondrial integrity and metabolism.

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“…Cytoplasmic and mitochondrial NAD/NADH ratios were calculated as follows: NAD/NADH = [oxidized substrate]/[reduced substrate] • 1/Keq, taking into account equilibrium constants for lactate, and βhydroxybutyrate dehydrogenases [47]. Linear regression analyses were made using the equation from…”

Section: Calculations and Statisticsmentioning

confidence: 99%

Role of Insulin/Glucagon Ratio and Cell Redox State in the Hyperglycaemia Induced by Exposure to Electromagnetic Fields in Rats.

Martiñón-Gutiérrez

Luna-Castro

Hernández‐Muñoz

2021

Preprint

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The exposure to extremely low-frequency electromagnetic fields (EMFs) with a might adversely affect the endocrine system and reproductive functions. Nonetheless, the use of EMFs in the form of magneto-therapy exerts beneficial actions in human medicine, but also affecting glucose and lipids metabolism, inducing hyperglycaemia and a “diabetic” response. Therefore, we used fed or fasted male rats for a single exposure to 60-Hz EMFs, determining serum levels of glucose, lipids, and indicators of cellular redox state and energy parameters. A single exposure to EMFs induced hyperglycaemia in both, fed and fasted rats, accompanied by an attenuated second serum insulin peak. In addition, EMFs also decreased serum levels of free fatty acids and lactate, while they increased those of pyruvate and acetoacetate. Changes in blood glucose level were related with a more oxidized cellular redox state and with the insulin/glucagon ratio. Moreover, rat’s glucose metabolism also coincided with variations in insulin and glucagon secretion, probably depending on cell redox state. The EMFs effects were not modified in animals previously subjected to a chronic exposure (14 days). In conclusion, increased serum glucose levels and glucose metabolism induced by EMFs exposure were closely related with the cellular redox state and with the insulin/glucagon ratio.

show abstract

Involvement of cell oxidant status and redox state in the increased non-enzymatic ethanol oxidation by the regenerating rat liver

Cited by 5 publications

References 52 publications

The Role of Insulin/glucagon Ratio and Cell Redox State in the Hyperglycemia Induced by Exposure to Pulsed Electromagnetic Fields in Rats

The Role of Insulin/glucagon Ratio and Cell Redox State in the Hyperglycemia Induced by Exposure to Pulsed Electromagnetic Fields in Rats

Involvement of Liver Redox State in the Zi-Dovudine-Induced Hepatic Steatosis and Its Partial Prevention by Adenosine

Role of Insulin/Glucagon Ratio and Cell Redox State in the Hyperglycaemia Induced by Exposure to Electromagnetic Fields in Rats.

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Involvement of cell oxidant status and redox state in the increased non-enzymatic ethanol oxidation by the regenerating rat liver

Cited by 5 publications

References 52 publications

The Role of Insulin/glucagon Ratio and Cell Redox State in the&nbsp;Hyperglycemia Induced by Exposure to Pulsed Electromagnetic Fields in Rats

The Role of Insulin/glucagon Ratio and Cell Redox State in the&nbsp;Hyperglycemia Induced by Exposure to Pulsed Electromagnetic Fields in Rats

Involvement of Liver Redox State in the Zi-Dovudine-Induced Hepatic Steatosis and Its Partial Prevention by Adenosine

Role of Insulin/Glucagon Ratio and Cell Redox State in the Hyperglycaemia Induced by Exposure to Electromagnetic Fields in Rats.

Contact Info

Product

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The Role of Insulin/glucagon Ratio and Cell Redox State in the Hyperglycemia Induced by Exposure to Pulsed Electromagnetic Fields in Rats

The Role of Insulin/glucagon Ratio and Cell Redox State in the Hyperglycemia Induced by Exposure to Pulsed Electromagnetic Fields in Rats