1987
DOI: 10.1128/jb.169.1.117-125.1987
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Involvement of chlA, E, M, and N loci in Escherichia coli molybdopterin biosynthesis

Abstract: All molybdenum enzymes except nitrogenase contain a common molybdenum cofactor, whose organic moiety is a novel pterin called molybdopterin (MPT). To assist in elucidating the biosynthetic pathway of MPT, two MPT-deficient mutants of Escherichia coli K-12 were isolated. They lacked activities of the molybdenum enzymes nitrate reductase and formate dehydrogenase, did not reconstitute apo nitrate reductase from a Neurospora crassa nit-1 strain, and did not yield form A, a derivative of MPT. By P1 mapping, these … Show more

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Cited by 102 publications
(100 citation statements)
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“…The activity of all three proteins depends on the presence of the C-terminal Gly-Gly motif, since deletion of this motif from either ThiS (32) or ubiquitin (33) deactivates the protein, and MoaD with either one more or one less glycine at the C terminus cannot complement a moaD Ϫ strain (34). Additionally, sequencing of the MoaD gene in the moaD -strain (MJ7chlM (20)) used here as a source of precursor Z revealed a single base change that results in conversion of the penultimate glycine to a glutamate (data not shown).…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…The activity of all three proteins depends on the presence of the C-terminal Gly-Gly motif, since deletion of this motif from either ThiS (32) or ubiquitin (33) deactivates the protein, and MoaD with either one more or one less glycine at the C terminus cannot complement a moaD Ϫ strain (34). Additionally, sequencing of the MoaD gene in the moaD -strain (MJ7chlM (20)) used here as a source of precursor Z revealed a single base change that results in conversion of the penultimate glycine to a glutamate (data not shown).…”
Section: Resultsmentioning
confidence: 99%
“…Mutations in either MPT synthase subunit or in MoeB result in accumulation of precursor Z within the affected organism. This has been observed in bacteria (18), humans (19), and Neurospora (20), and such mutants can serve as experimental sources of precursor Z (21).…”
Section: Molybdopterin (Mpt)mentioning
confidence: 99%
“…Bacterial Strains, Media, and Growth Conditions-The E. coli moaD(DE3) and moaE (RK5204) mutant strains used in this study were described previously (20,21). E. coli BL21(DE3) cells and pET15b were obtained from Novagen.…”
Section: Methodsmentioning
confidence: 99%
“…Bacterial Strains-E. coli chlAI, chlN, and chlM strains, now designated moaA Ϫ , moeB Ϫ , and moaD Ϫ , are isogenic mutants that were described previously (10). E. coli BL21(DE3) cells were obtained from Novagen.…”
Section: Methodsmentioning
confidence: 99%