Involvement of cholesterol, calcium and progesterone in the induction of capacitation and acrosome reaction of mammalian spermatozoa

Witte, Tanja Semira; Schäfer-Somi, Sabine

doi:10.1016/j.anireprosci.2007.07.007

Cited by 104 publications

(67 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In many species, progesterone is present in the preovulatory follicular fluid and released to the oviduct during ovulation, which might be one of the sources of local progesterone at the site of fertilization (Osman et al, 1989;Thomas and Meizel, 1989). The cumulus cells have also been reported to secret progesterone after ovulation, which is thought to be involved in both sperm chemotaxis to the cumulus oophorus and cumulus-induced acrosome reaction (Witte and Schäfer-Somi, 2007;Guidobaldi et al, 2008). Therefore progesterone has been suggested to be the trigger of AR in the cumulus layer in vivo.…”

Section: Involvement Of a Sperm-released Factor In Induction Of Ar Bymentioning

confidence: 99%

Acrosome reaction in the cumulus oophorus revisited: involvement of a novel sperm-released factor NYD-SP8

2011

View full text Add to dashboard Cite

Fertilization is a process involving multiple steps that lead to the final fusion of one sperm and the oocyte to form the zygote. One of the steps, acrosome reaction (AR), is an exocytosis process, during which the outer acrosome membrane fuses with the inner sperm membrane, leading to the release of acrosome enzymes that facilitate sperm penetration of the egg investments. Though AR has been investigated for decades, the initial steps of AR in vivo, however, remain largely unknown. A well elucidated model holds the view that AR occurs on the surface of the zona pellucida (ZP), which is triggered by binding of sperm with one of the ZP glycosylated protein, ZP3. However, this model fails to explain the large number of 'falsely' acrosome-reacted sperms found within the cumulus layer in many species examined. With the emerging evidence of cross-talk between sperm and cumulus cells, the potential significance of AR in the cumulus oophorus, the outer layer of the egg, has been gradually revealed. Here we review the acrosome status within the cumulus layer, the cross-talk between sperm and cumulus cells with the involvement of a novel spermreleased factor, NYD-SP8, and re-evaluate the importance and physiological significance of the AR in the cumulus in fertilization.

show abstract

Section: Involvement Of a Sperm-released Factor In Induction Of Ar Bymentioning

confidence: 99%

Acrosome reaction in the cumulus oophorus revisited: involvement of a novel sperm-released factor NYD-SP8

2011

View full text Add to dashboard Cite

show abstract

“…Cholesterol efflux from the plasma membrane is one of the recognised responses of spermatozoa (see section 'Physiological alterations of the sperm surface') in a capacitation-supportive medium (Travis & Kopf 2002, Witte & Schafer-Somi 2007. While these modifications are thought to be essential for fertilisation (Parinaud et al 2000), they also increase the fragility of the sperm surface, rendering it more sensitive to handling imposed deterioration (Van Gestel et al 2005b).…”

Section: Stabilisation Of the Sperm Surface With Cholesterol And Othementioning

confidence: 99%

Sperm surface changes and physiological consequences induced by sperm handling and storage

2011

View full text Add to dashboard Cite

Spermatozoa interact with their immediate environment and this contact remodels the sperm surface in preparation for fertilisation. These fundamental membrane changes will be critically covered in this review with special emphasis on the very specific surface destabilisation event, capacitation. This process involves very subtle and intricate modifications of the sperm membrane including removal of suppression (decapacitation) factors and changes in the lateral organisation of the proteins and lipids of the sperm surface. Processing of sperm for assisted reproduction (storage, sex-sorting, etc.) subjects spermatozoa to numerous stressors, and it is possible that this processing overrides such delicate processes resulting in sperm instability and cell damage. To improve sperm quality, novel mechanisms must be used to stabilise the sperm surface during handling. In this review, different types of membrane stress are considered, as well as novel surface manipulation methods to improve sperm stability.

show abstract

“…Along with phospholipids, cholesterol is necessary for cell physical integrity and ensures fluidity of the cell membrane. Cholesterol plays a special role in the sperm membrane because its release from the sperm membrane initiates the key step in the process of capacitation and acrosome reaction that is crucial for fertilization (Witte andSchafer-Somi, 2007, Srivastava et al, 2013). Moreover, adding cholesterol to diluents prior to defreezing increases sperm resistance to stress caused by the freezing-defreezing procedures, preserving sperm motility and fertilization potential (Moore et al, 2005).…”

Section: Resultsmentioning

confidence: 99%

Effect of addition of trehalose on the liquid storage (5°C) of mithun (<italic>Bos frontalis</italic>) semen

Perumal

Vüpru

Rajkhowa

2015

IJAR

View full text Add to dashboard Cite

The present study was undertaken to assess the effect of trehalose on sperm parameters, enzymatic profiles and biochemical profiles of seminal plasma of mithun. The semen ejaculates were collected from mithun and was split into four groups as group 1: semen without additives (control), group 2 to 4: semen with 50 mM, 75 mM and 100 mM of trehalose, respectively in EYTC extender. These parameters were assessed at 5 o C for 0, 6, 12, 24 and 30 h of incubation. Inclusion of trehalose resulted in significant (p < 0.05) decrease in percentages of dead spermatozoa, abnormal spermatozoa and acrosomal abnormalities as compared with control group. Additionally, trehalose at 50 mM has significant improvement in quality of mithun semen in in-vitro storage. It was due to the protective effects of trehalose on sperm parameters as sperm membrane stabilizer and prevents efflux of cholesterol, lipid peroxide formation and biochemical enzymes from sperm cell during preservation.

show abstract

Involvement of cholesterol, calcium and progesterone in the induction of capacitation and acrosome reaction of mammalian spermatozoa

Cited by 104 publications

References 62 publications

Acrosome reaction in the cumulus oophorus revisited: involvement of a novel sperm-released factor NYD-SP8

Acrosome reaction in the cumulus oophorus revisited: involvement of a novel sperm-released factor NYD-SP8

Sperm surface changes and physiological consequences induced by sperm handling and storage

Effect of addition of trehalose on the liquid storage (5°C) of mithun (<italic>Bos frontalis</italic>) semen

Contact Info

Product

Resources

About