Involvement of Cytochrome P450 in Pentachlorophenol Transformation in a White Rot Fungus Phanerochaete chrysosporium

Ning, Daliang; Wang, Hui

doi:10.1371/journal.pone.0045887

Cited by 38 publications

(12 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In P. chrysosporium , six PAH-responsive CYP genes identified by genome-wide microarray screening were induced and upregulated upon varying ring-sized PAHs, and their catalytic function towards various PAHs was determined using recombinant enzymes [ 51 ]. Furthermore, the induction of CYPs by pentachlorophenol (PCP) was also observed in P. chrysosporium , in which only the PCP-induced fungal microsomes led to the oxidation of PCP to form tetrachlorohydroquinone [ 68 ]. Remarkably, the transcriptional regulation of CYP-dependent metabolic pathways can be alternatively induced or upregulated by defined high or low nitrogen conditions, as observed in P. chrysosporium, C. versicolor, P. placenta , and A. oryzae [ 40 , 69 , 70 ].…”

Section: Systematic Approach To Heterologous Expression Of Fungal Cypmentioning

confidence: 99%

Versatile biocatalysis of fungal cytochrome P450 monooxygenases

2016

View full text Add to dashboard Cite

Cytochrome P450 (CYP) monooxygenases, the nature’s most versatile biological catalysts have unique ability to catalyse regio-, chemo-, and stereospecific oxidation of a wide range of substrates under mild reaction conditions, thereby addressing a significant challenge in chemocatalysis. Though CYP enzymes are ubiquitous in all biological kingdoms, the divergence of CYPs in fungal kingdom is manifold. The CYP enzymes play pivotal roles in various fungal metabolisms starting from housekeeping biochemical reactions, detoxification of chemicals, and adaptation to hostile surroundings. Considering the versatile catalytic potentials, fungal CYPs has gained wide range of attraction among researchers and various remarkable strategies have been accomplished to enhance their biocatalytic properties. Numerous fungal CYPs with multispecialty features have been identified and the number of characterized fungal CYPs is constantly increasing. Literature reveals ample reviews on mammalian, plant and bacterial CYPs, however, modest reports on fungal CYPs urges a comprehensive review highlighting their novel catalytic potentials and functional significances. In this review, we focus on the diversification and functional diversity of fungal CYPs and recapitulate their unique and versatile biocatalytic properties. As such, this review emphasizes the crucial issues of fungal CYP systems, and the factors influencing efficient biocatalysis.

show abstract

Section: Systematic Approach To Heterologous Expression Of Fungal Cypmentioning

confidence: 99%

Versatile biocatalysis of fungal cytochrome P450 monooxygenases

2016

View full text Add to dashboard Cite

show abstract

“…Extracellular Peroxidases and laccase have been reported to be the key enzymes involved in the degradation of recalcitrant aromatic polymers by WRF (Mester and Tien 2000; Zahmatkesh et al 2010; Mendonça Maciel et al 2010). Cytochrome P450 (CYP) enzymes comprise another group of enzymes of which their involvement in the degradation of some organic compounds by WRF has been demonstrated (Ning and Wang 2012; Kelly and Kelly 2013; Zhang et al 2015). CYPs are membrane-bound hemoproteins that catalyze hydroxylation, epoxidation and monooxygenation reactions (Neve and Ingelman-Sundberg 2008; Aranda 2016).…”

Section: Introductionmentioning

confidence: 99%

Bioremoval of humic acid from water by white rot fungi: exploring the removal mechanisms

et al. 2016

View full text Add to dashboard Cite

Twelve white rot fungi (WRF) strains were screened on agar plates for their ability to bleach humic acid (HA). Four fungal strains were selected and tested in liquid media for removal of HA. Bioremediation was investigated by HA color removal and changes in the concentration and molecular size distribution of HA by size exclusion chromatography. Trametes versicolor and Phanerochaete chrysosporium showed the highest HA removal efficiency, reaching about 80%. Laccase and manganese peroxidase were measured as extracellular enzymes and their relation to the HA removal by WRF was investigated. Results indicated that nitrogen limitation could enhance the WRF extracellular enzyme activity, but did not necessarily increase the HA removal by WRF. The mechanism of bioremediation by WRF was shown to involve biosorption of HA by fungal biomass and degradation of HA to smaller molecules. Also, contradicting previous reports, it was shown that the decolorization of HA by WRF could not necessarily be interpreted as degradation of HA. Biosorption experiments revealed that HA removal by fungal biomass is dependent not only on the amount of biomass as the sorbent, but also on the fungal species. The involvement of cytochrome P450 (CYP) enzymes was confirmed by comparing the HA removal capability of fungi with and without the presence of a CYP inhibitor. The ability of purified laccase from WRF to solely degrade HA was proven and the importance of mediators was also demonstrated.

show abstract

“…These genes play a role in the adaptation of fungi to lignin decomposition [38]. P450 mediated pentachlorophenol oxidation [39], the CYP512, CYP5136 and CYP5150 families can oxidize a wide range of hydrocarbons, including phytochemicals and key metabolic intermediates in fungi, such as steroids, indicating that they play a role in the primary and secondary metabolism of fungi [40]. Matsuzaki et al studied the intracellular changes of P. chrysosporium during benzoic acid metabolism at the proteome and metabolic level, and found that aryl alcohol dehydrogenase, aromatic aldehyde dehydrogenase and cytochrome P450 play a key role in benzoic acid metabolism [31].…”

Section: Differential Gene Expression As a Function Of Wood Stimulusmentioning

confidence: 99%

Transcriptomics analysis and co-expression network revealing candidate genes for laccase activity of Lenzites gibbosa

Chen¹,

Chi²,

Zhao³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Lenzites gibbosa is a common white-rot fungus of Polyporaceae in the cold temperate zone which cause spongy white decay of wood. Results In this study, the lignin degradation pathway of L. gibbosa at 5-time points under wood treatment was studied by RNA-Req technology. A total of 5232 DEGs were identified from 15 libraries. In 0–3 days, the mycelia are in the adaptive stage, and the mycelia began to proliferate within 3–5 days. After 5 days, the number of DGEs decreased significantly, the mycelium growth entered the platform stage, and the life activity was basically stable. In the secondary metabolism, oxidoreductase such as laccase, 2-oxoglutarate-Fe (II) type oxidoreductase, peroxisomal hydratase-dehydrogenase, dual-functional monooxygenase dominated and increased steadily, and manganese peroxidase appeared in the middle stage. With the accumulation of lignin intermediate products, P450 and ABC transporters were from the inhibition to the activation. Weighted gene co-expression network analysis (WGCNA) showed that among the 24 modules 6 modules were significantly correlated with laccase activity and the most correlated were turquoise and blue module. The central hub genes were also identified, including gene_7458, gene_61, gene_7458, gene_1741, gene_11087 which were consistent with the DGEs. These genes have high connectivity, module membership, and gene significance in the module. The enrichment analysis of GO and KEGG pathway indicated that the genes involved in cell cycle, citrate cycle (TCA cycle), nicotinate and nicotinamide metabolism, mitochondrial outer membrane, succinate dehydrogenase, carboxypeptidase and exopeptidase activity, flavin adenine dinucleotide binding, oxidoreductase activity, acting on the CH-CH group of donors, quinone or related compound as acceptor pathway were highly related to laccase synthesis pathway. Construction of gene co-expression network and hypothetical L. gibbosa laccase synthesis pathway. Conclusion This study focused on the screening of L. gibbosa degradation of lignin-related genes. And, this is the first study reporting co-expression patterns of a gene network in L. gibbosa laccase activity after wood treatment which is helpful to understand the synthesis pathway of laccase and improve the activity of laccase, which can be used to increase the rate of lignin degradation by L. gibbosa in the further.

show abstract

Involvement of Cytochrome P450 in Pentachlorophenol Transformation in a White Rot Fungus Phanerochaete chrysosporium

Cited by 38 publications

References 50 publications

Versatile biocatalysis of fungal cytochrome P450 monooxygenases

Versatile biocatalysis of fungal cytochrome P450 monooxygenases

Bioremoval of humic acid from water by white rot fungi: exploring the removal mechanisms

Transcriptomics analysis and co-expression network revealing candidate genes for laccase activity of Lenzites gibbosa

Contact Info

Product

Resources

About