Involvement of ER stress in apoptosis induced by sialic acid-binding lectin (leczyme) from bullfrog eggs

Tatsuta, Takeo; Hosono, Masahiro; Miura, Yuki; Sugawara, Shigeki; Kariya, Yukiko; Hakomori, Sen‐itiroh; Nitta, Kazuo

doi:10.3892/ijo.2013.2128

Cited by 23 publications

(22 citation statements)

References 39 publications

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“…cSBL is cytotoxic to cancer cells including leukemia [18–21], breast carcinoma [21–24], mesothelioma [25], and hepatoma cells [21, 26, 27]. It has little effect on normal cells such as fibroblasts, melanocytes, keratinocytes, and mesothelial cells [20, 21, 25, 26, 28].…”

Section: Introductionmentioning

confidence: 99%

Synergistic anti-tumor effect of bullfrog sialic acid-binding lectin and pemetrexed in malignant mesothelioma

et al. 2017

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Malignant mesothelioma is an aggressive cancer with limited therapeutic options. Sialic acid-binding lectin isolated from Rana catesbeiana oocytes (cSBL) is a multifunctional protein with anti-cancer activity. The effects of pemetrexed, cisplatin, and cSBL were evaluated in mesothelioma and normal mesothelial cell lines. We evaluated cytotoxicity, apoptosis, caspase-3 cleavage and activation, cell proliferation, cell cycle arrest, and levels of cell cycle proteins in H28 cells treated with pemetrexed, cisplatin, and cSBL alone or in combination. Treatment with cSBL alone was cytotoxic to mesothelioma cells. The anti-cancer effect of cSBL was observed in a broader range of cell lines and exhibited greater cancer cell selectivity than pemetrexed or cisplatin. Combination treatment with pemetrexed + cSBL resulted in greater dose-dependent cytotoxicity than pemetrexed + cisplatin, the standard of care in mesothelioma. The synergistic effect of pemetrexed + cSBL was mediated by the cytostatic effect of pemetrexed and the cytotoxic effect of cSBL. It thus appears that cSBL has therapeutic potential for the treatment of mesothelioma.

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Section: Introductionmentioning

confidence: 99%

Synergistic anti-tumor effect of bullfrog sialic acid-binding lectin and pemetrexed in malignant mesothelioma

et al. 2017

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“…Just as in a jigsaw puzzle, many studies have tried to elucidate the proper molecular mechanism of action of SBLc. The pieces of the puzzles are a tumor-specific mechanism of action, the activation of the intrinsic apoptotic pathway with the involvement of heat shock protein 70 (HSP70) and the phosphorylation of p38 MAPK [108,110,116,120,134,135]. The role of the lectin part of SBLc is to select cancer cells through the binding of specific sialic acid carbohydrates residues; then, specific receptors allow it to penetrate the cells, where the RNAse activity promotes the cleavage of RNA.…”

Section: Rana Catesbeianamentioning

confidence: 99%

“…On MCF-7 estrogen receptor (ER)-positive breast cancer cells and H28 malignant mesothelioma cells, SBLc induced Bcl-2-mediated cell death [116]. On Jurkat cells, it promoted endoplasmic reticulum stress that may or may not be involved in its antitumor activity [134]. On p388 mouse lymphoma cells, HSP70 was linked to its ability to cause apoptosis.…”

Section: Rana Catesbeianamentioning

confidence: 99%

Antitumor Potential of Marine and Freshwater Lectins

et al. 2019

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Often, even the most effective antineoplastic drugs currently used in clinic do not efficiently allow complete healing due to the related toxicity. The reason for the toxicity lies in the lack of selectivity for cancer cells of the vast majority of anticancer agents. Thus, the need for new potent anticancer compounds characterized by a better toxicological profile is compelling. Lectins belong to a particular class of non-immunogenic glycoproteins and have the characteristics to selectively bind specific sugar sequences on the surface of cells. This property is exploited to exclusively bind cancer cells and exert antitumor activity through the induction of different forms of regulated cell death and the inhibition of cancer cell proliferation. Thanks to the extraordinary biodiversity, marine environments represent a unique source of active natural compounds with anticancer potential. Several marine and freshwater organisms, ranging from the simplest alga to the most complex vertebrate, are amazingly enriched in these proteins. Remarkably, all studies gathered in this review show the impressive anticancer effect of each studied marine lectin combined with irrelevant toxicity in vitro and in vivo and pave the way to design clinical trials to assess the real antineoplastic potential of these promising proteins. It provides a concise and precise description of the experimental results, their interpretation as well as the experimental conclusions that can be drawn.

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“…Furthermore, a direct causality between SR stress and muscle detriment has also been proposed since mitigating SR stress can partially restore muscle mass and/or strength in experimental animal models (13,14).We have previously shown that the muscle weakness in COPD is associated with reduction in serum sialic acid (15), a monosaccharide with various biological functions. Sialic acid has involvement in SR stress and apoptosis (16) while deficiency of it leads to oxidative stress and atrophy in skeletal muscle (17).…”

Section: Introductionmentioning

confidence: 99%

SR stress in locomotor muscles of patients with chronic obstructive pulmonary disease

Qaisar

Qayyum

Muhammad

2020

Preprint

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Background The potential contribution of chronic dysregulation of sarcoplasmic reticulum (SR) protein homeostasis (a condition called SR stress) to skeletal muscle loss is poorly understood. We investigated the degree of activation of SR stress in locomotor muscles of patients with chronic obstructive pulmonary disease (COPD), a respiratory disease with systemic manifestations. Methods We analyzed the markers of SR stress and associated pathologies in vastus lateralis muscles of 60-65 years old male healthy controls and patients with mild (COPD stages 1 & 2) and advanced (COPD stages 3 & 4) COPD (N = 6-8 / group). Results Skeletal muscle proteins expressions of GRP94, BiP, CHOP and ATF were significantly elevated in advanced COPD (≈53%, ≈3.6 fold, ≈3.5 fold and ≈3.2 fold, respectively) compared with healthy controls. The expression of downstream markers of SR stress including apoptosis, inflammation and autophagy was increased, while the maximal activity of SR Ca2+ ATPase (SERCA) enzyme was significantly reduced in advanced COPD (≈41%) than healthy controls. Single muscle fiber diameter and cytoplasmic domain per myonucleus were significantly smaller (≈14% and 13%, respectively) in patients with advanced COPD than healthy controls. These changes in SR dysfunction were accompanied by substantially elevated levels of global oxidative stress including lipid peroxidation and mitochondrial ROS production. Conclusion Taken together, our data suggests that the muscle weakness in advanced COPD is in part driven by elevated SR stress and its pathological consequences. The data provided can lead to potential therapeutic interventions of SR dysfunction for muscle detriment in COPD.

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Involvement of ER stress in apoptosis induced by sialic acid-binding lectin (leczyme) from bullfrog eggs

Cited by 23 publications

References 39 publications

Synergistic anti-tumor effect of bullfrog sialic acid-binding lectin and pemetrexed in malignant mesothelioma

Synergistic anti-tumor effect of bullfrog sialic acid-binding lectin and pemetrexed in malignant mesothelioma

Antitumor Potential of Marine and Freshwater Lectins

SR stress in locomotor muscles of patients with chronic obstructive pulmonary disease

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