Involvement of exogenous H2S in recovery of cardioprotection from ischemic post-conditioning via increase of autophagy in the aged hearts

Chen, Junting; Gao, Jun; Sun, Weiyi; Li, Lina; Wang, Yuehong; Bai, Shun; Li, Xiao-Xue; Wang, Rui; Wu, Lingyun; Li, Hongzhu; Xu, Chi

doi:10.1016/j.ijcard.2016.06.200

Cited by 72 publications

(56 citation statements)

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“…Although the physiological effect of H 2 S on the heart 20–22 , and the underlying molecular mechanisms are documented 10, 23–25 , the differential effect of H 2 S or Hcy on CBS and CSE expressions, the cross-talk/feedback regulation of CBS and CSE, and the underlying molecular mechanism by which H 2 S regulates CSE are unclear. The present study fill-in the gap of knowledge in the field of H 2 S and Hcy biology by answering these questions.…”

Section: Discussionmentioning

confidence: 99%

H2S and homocysteine control a novel feedback regulation of cystathionine beta synthase and cystathionine gamma lyase in cardiomyocytes

Nandi

Mishra

2017

Sci Rep

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Hydrogen sulfide (H2S), a cardioprotective gas, is endogenously produced from homocysteine by cystathionine beta synthase (CBS) and cystathionine gamma lyase (CSE) enzymes. However, effect of H2S or homocysteine on CBS and CSE expression, and cross-talk between CBS and CSE are unclear. We hypothesize that homocysteine and H2S regulate CBS and CSE expressions in a dose dependent manner in cardiomyocytes, and CBS deficiency induces cardiac CSE expression. To test the hypothesis, we treated murine atrial HL1 cardiomyocytes with increasing doses of homocysteine or Na2S/GYY4137, a H2S donor, and measured the levels of CBS and CSE. We found that homocysteine upregulates CSE but downregulates CBS whereas Na2S/GYY4137 downregulates CSE but upregulates CBS in a dose-dependent manner. Moreover, the Na2S-treatment downregulates specificity protein-1 (SP1), an inducer for CSE, and upregulates miR-133a that targets SP1 and inhibits cardiomyocytes hypertrophy. Conversely, in the homocysteine-treated cardiomyocytes, CBS and miR-133a were downregulated and hypertrophy was induced. In vivo studies using CBS+/−, a model for hyperhomocysteinemia, and sibling CBS+/+ control mice revealed that deficiency of CBS upregulates cardiac CSE, plausibly by inducing SP1. In conclusion, we revealed a novel mechanism for H2S-mediated regulation of homocysteine metabolism in cardiomyocytes, and a negative feedback regulation between CBS and CSE in the heart.

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Section: Discussionmentioning

confidence: 99%

H2S and homocysteine control a novel feedback regulation of cystathionine beta synthase and cystathionine gamma lyase in cardiomyocytes

Nandi

Mishra

2017

Sci Rep

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“…The hydrogen sulphide (H 2 S) donor, sodium hydrosulphide (NaHS), has shown effectiveness in both in vitro and in vivo studies involving d ‐galactose‐induced ageing heart models . In the in vitro study, 10 g/l of d ‐galactose was added to the H9C2 cells for 48 hr to establish an ageing model and subjected to 3‐hr hypoxia/6‐hr reoxygenation.…”

Section: Effects Of Therapeutic Interventions On the D‐galactose‐indumentioning

confidence: 99%

Effects of d‐galactose‐induced ageing on the heart and its potential interventions

Bo-Htay

Palee

Apaijai

et al. 2018

J Cellular Molecular Medi

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Ageing is a strong independent risk factor for disability, morbidity and mortality. Post‐mitotic cells including those in the heart are a particular risk to age‐related deterioration. As the occurrence of heart disease is increasing rapidly with an ageing population, knowledge regarding the mechanisms of age‐related cardiac susceptibility and possible therapeutic interventions needs to be acquired to prevent advancing levels of heart disease. To understand more about the ageing heart, numerous aged animal models are being used to explore the underlying mechanisms. Due to time‐consuming for investigations involving naturally aged animals, mimetic ageing models are being utilized to assess the related effects of ageing on disease occurrence. d‐galactose is one of the substances used to instigate ageing in various models, and techniques involving this have been widely used since 1991. However, the mechanism through which d‐galactose induces ageing in the heart remains unclear. The aim of this review was to comprehensively summarize the current findings from in vitro and in vivo studies on the effects of d‐galactose‐induced ageing on the heart, and possible therapeutic interventions against ageing heart models. From this review, we hope to provide invaluable information for future studies and based on the findings from experiments involving animals, we can inform possible therapeutic strategies for the prevention of age‐related heart diseases in clinical settings.

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“…Furthermore it is well established that enhancing brain autophagy contributes to the antidepressant‐like properties of trehalose . Simultaneously, emerging evidence has indicated that exogenous H 2 S protects the heart and renal organs against ischaemic‐reperfusion injury in rats via increasing autophagy . Interestingly, it has been revealed that BDNF promotes neuron survival through improving autophagy and that fluoxetine exerts its antidepressant effect through enhancing autophagic pathway by upregulation of the BDNF‐TrkB pathway .…”

Section: Introductionmentioning

confidence: 99%

“…21 Simultaneously, emerging evidence has indicated that exogenous H 2 S protects the heart and renal organs against ischaemic-reperfusion injury in rats via increasing autophagy. 22,23 Interestingly, it has been revealed that BDNF promotes neuron survival through improving autophagy 24 and that fluoxetine exerts its antidepressant effect through enhancing autophagic pathway by upregulation of the BDNF-TrkB pathway. 25 Therefore, we would like to investigate whether the contribution of the BDNF-TrkB pathway to antidepressant-like effects of H 2 S in diabetic rats is achieved by enhancing hippocampal autophagy.…”

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confidence: 99%

BDNF‐TrkB pathway mediates antidepressant‐like roles of H₂S in diabetic rats via promoting hippocampal autophagy

Liu

Wei

et al. 2019

Clin Exp Pharma Physio

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Hydrogen sulfide (H2S) plays antidepressant‐like roles in diabetic rats. However, the underlying mechanisms remain unclear. Brain‐derived neurotropic factor (BDNF), a neurotrophic factor, plays important regulatory roles in depression by its high‐affinity tropomysin‐related kinase B (TrkB) receptor. Autophagy also is implicated in modulation of depression. Previous work confirmed the modulatory roles of H2S in BDNF protein expression and autophagy. Thus, in this study, we explored whether the BDNF‐TrkB pathway mediates the antidepressant‐like effects of H2S in diabetic rats and whether this process is achieved via promoting hippocampal autophagy. We demonstrated that H2S upregulated the expressions of BDNF and p‐TrkB proteins in the hippocampus of streptozotocin (STZ)‐induced diabetic rats. K252a (an inhibitor of BDNF‐TrkB pathway) reversed the antidepressant‐like roles of H2S, as evidenced by the tail suspension, forced swimming, novelty suppressed feeding, and elevated plus‐maze tests. Furthermore, K252a abolished H2S‐promoted hippocampal autophagy in diabetic rats, as evidenced by a decrease in the number of autolysosome, downregulation of Beclin‐1 (a regulator of autophagy in the early stage of the formation of autophagosomal membranes and its level is positively correlated with autophagic activity) expression, and upregulation of P62 (a substrate of autophagic degradation and its level is inversely correlated with autophagic activity) expression, in the hippocampus of rats co‐treated with NaHS and STZ. Taken together, these data indicated that the BDNF‐TrkB pathway mediates the antidepressant‐like roles of H2S in diabetic rats by enhancing hippocampal autophagy.

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Involvement of exogenous H2S in recovery of cardioprotection from ischemic post-conditioning via increase of autophagy in the aged hearts

Cited by 72 publications

References 50 publications

H2S and homocysteine control a novel feedback regulation of cystathionine beta synthase and cystathionine gamma lyase in cardiomyocytes

H2S and homocysteine control a novel feedback regulation of cystathionine beta synthase and cystathionine gamma lyase in cardiomyocytes

Effects of d‐galactose‐induced ageing on the heart and its potential interventions

BDNF‐TrkB pathway mediates antidepressant‐like roles of H₂S in diabetic rats via promoting hippocampal autophagy

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Involvement of exogenous H2S in recovery of cardioprotection from ischemic post-conditioning via increase of autophagy in the aged hearts

Cited by 72 publications

References 50 publications

H2S and homocysteine control a novel feedback regulation of cystathionine beta synthase and cystathionine gamma lyase in cardiomyocytes

H2S and homocysteine control a novel feedback regulation of cystathionine beta synthase and cystathionine gamma lyase in cardiomyocytes

Effects of d‐galactose‐induced ageing on the heart and its potential interventions

BDNF‐TrkB pathway mediates antidepressant‐like roles of H2S in diabetic rats via promoting hippocampal autophagy

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BDNF‐TrkB pathway mediates antidepressant‐like roles of H₂S in diabetic rats via promoting hippocampal autophagy