Involvement of free radicals followed by the activation of phospholipase A2 in the mechanism that underlies the combined effects of methamphetamine and morphine on subacute toxicity or lethality in mice: Comparison of the therapeutic potential of fullerene, mepacrine, and cooling

Mori, Tomohisa; Ito, Shinobu; Namiki, Mizuho; Suzuki, Tadashi; Kobayashi, Shizuko; Matsubayashi, Kenji; Sawaguchi, Toshiko

doi:10.1016/j.tox.2007.03.027

Cited by 16 publications

(8 citation statements)

References 30 publications

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“…Besides they inhibit allergic response (Ryan et al, 2007), potentiate hair growth , improve cognitive function and increase lifespan of mammals (Quick et al, 2008). Pristine C 60 fullerene has prominent potential to attenuate toxicity and to eliminate the increase in the HO-radicals induced by the coadministration of methamphetamine plus morphine as an antiradical agent (Mori et al, 2007). Efficacy of carboxyfullerene to suppress acrilamide toxicity in human neuroblastoma cells in vitro (Sumizawa and Igisu, 2009) as well as protective effects of polyhydroxyfullerene (fullerenol) against doxorubicin-induced nephro-, pulmo-, cardio-, and hepatotoxicity (Srdjenovic et al, 2010) in rats have been also attributed to their antioxidant activity.…”

Section: Fatty Acidsmentioning

confidence: 99%

Protective effects of nanostructures of hydrated C60 fullerene on reproductive function in streptozotocin-diabetic male rats

et al. 2011

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Diabetes mellitus is a well-recognized cause of male sexual dysfunction and impairments of male fertility. Streptozotocin (STZ) is used for medical treatment of neoplastic islet β-cells of pancreas and producing of animal model of diabetes mellitus type 1 that is characterized by suppression of reproductive activity due to the hyperglycaemia-induced oxidative stress and histopathological alterations in testes. Seeking for the agents that could alleviate diabetes-induced damage to reproductive system is yet the important area of inquiry. The present study was designed to evaluate whether hydrated C(60) fullerene (C(60)HyFn), which is known to be powerful bioantioxidant, eliminate testicular dysfunction induced by STZ-diabetes in rats. Wistar strain male albino rats were divided into four groups of six animals each: (1) control group, (2) C(60)HyFn-treated nondiabetic group, (3) STZ-diabetic group and (4) C(60)HyFn-treated diabetic group. Once hyperglycaemia was induced by STZ, rats in the second and fourth groups were treated with C(60)HyFn (in the form of drinking water) at the dose of 4μg/kg daily for 5 weeks. In diabetic rats, relative weights of right cauda epididymis, seminal vesicles, prostate, sperm motility and epididymal sperm concentration were significantly less than those of control group, but which were restored in the fourth group treated with C(60)HyFn (p<0.001). In hematoxylin and eosin staining, marked histopathological changes including degeneration, desquamation, disorganisation and reduction in germinal cells, interstitial oedema and congestion were evident in the testis of diabetic rats, but C(60)HyFn treatment resulted in recovery of histopathological changes and an increase in Johnsen's testicular score significantly (p<0.001). C(60)HyFn treatment restores the increased apoptosis induced by STZ-diabetes. In diabetic rats, levels of serum testosterone, testicular reduced glutathione (GSH) and alpha-tocopherol were significantly reduced and testicular lipid peroxidation level was increased (p<0.001). Nevertheless, treatment of diabetic rats with C(60)HyFn resulted in significant corrective effects on these parameters towards the control levels. C(60)HyFn, applied alone, did not exert any toxic effects in testicular tissues. Furthermore, C(60)HyFn treatment in diabetic and nondiabetic rats resulted in considerable elevations of some important polyunsaturated fatty acids. In conclusion, we have presented for the first time substantial evidence that administration of C(60)HyFn significantly reduces diabetes-induced oxidative stress and associated complications such as testicular dysfunction and spermatogenic disruption.

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Section: Fatty Acidsmentioning

confidence: 99%

Protective effects of nanostructures of hydrated C60 fullerene on reproductive function in streptozotocin-diabetic male rats

et al. 2011

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“…I.p. injection of C 60 fullerenes has been used in some investigations as a route of exposure in order to focus on C 60 fullerene's ability to prevent toxic effects of carbon tetrachloride [ 20 ] and methamphetamine and morphine [ 21 ]. We used the i.p.…”

Section: Introductionmentioning

confidence: 99%

Modest vasomotor dysfunction induced by low doses of C60 fullerenes in apolipoprotein E knockout mice with different degree of atherosclerosis

et al. 2009

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“…Applications of fullerenes for correction of the negative consequences of narcotic and neurotropic agents are obviously prospective. This is supported by experimental data on the efficacy of a solubilized form of С 60 as a neuroprotective means in morphine and met-amphetamine intoxications [43]. We found in our previous study that introduction of the hydrated form of fullerene to experimental animals within the entire period of alcoholization led to a decrease in the level of oxidative impairments of lipids and proteins in the nerve tissue and promoted recovery of the astroglial function [16].…”

Section: Discussionmentioning

confidence: 60%

Disorders in the Cytoskeleton of Astroglia and Neurons in the Rat Brain Induced by Long-Lasting Exposure to Ethanol and Correction of These Shifts by Hydrated Fullerene С60

2008

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Using an immunoblotting technique, we examined the content of proteins of intermediate filaments of the cytoskeleton of neurons and astroglial cells and also changes in the polypeptide composition of these proteins in different brain regions of rats subjected to long-term (12 weeks) alcoholization. The sensitivity of these indices to the effect of ethanol in different cerebral structures was in the following sequence: hippocampus > cerebral cortex > cerebellum. The greatest changes in a marker of the astrocyte cytoskeleton (glial fibrillary acidic protein, GFAP) were observed in the hippocampus of alcoholized animals, where the GFAP level was by 72% lower with respect to the control values. In this cerebral region, the content of the neurofilament 210-kdalton subunit also sharply dropped (by 76% with respect to the control). A positive correlation between a decrease in the GFAP content and loss of the neurofilament 210-kdalton subunit was demonstrated. These data show that the organization of the intracellular filamentary system of neurons and gliocytes is disturbed under experimental conditions, and this is one of the probable reasons for cell death in the nerve tissue induced by chronic consumption of ethanol. The use of a hydrated form of fullerene С 60 (its molecular/colloid solution) for antioxidant correction of the pathological state of the CNS induced by the above-mentioned toxicant removed, to a considerable extent, negative modifications of cytoskeletal structures and protected astroglial and nerve cells from degeneration.

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Involvement of free radicals followed by the activation of phospholipase A2 in the mechanism that underlies the combined effects of methamphetamine and morphine on subacute toxicity or lethality in mice: Comparison of the therapeutic potential of fullerene, mepacrine, and cooling

Cited by 16 publications

References 30 publications

Protective effects of nanostructures of hydrated C60 fullerene on reproductive function in streptozotocin-diabetic male rats

Protective effects of nanostructures of hydrated C60 fullerene on reproductive function in streptozotocin-diabetic male rats

Modest vasomotor dysfunction induced by low doses of C60 fullerenes in apolipoprotein E knockout mice with different degree of atherosclerosis

Disorders in the Cytoskeleton of Astroglia and Neurons in the Rat Brain Induced by Long-Lasting Exposure to Ethanol and Correction of These Shifts by Hydrated Fullerene С60

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