Involvement of GABA and opioid peptide receptors in sevoflurane-induced antinociception in rat spinal cord

Wang, Yingwei; Deng, Xiaoming; You, Xinru; Liu, Shu-Xiao

doi:10.1111/j.1745-7254.2005.00175.x

Cited by 15 publications

(5 citation statements)

References 17 publications

(29 reference statements)

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“…Previous laboratory studies have demonstrated that the intraperitoneal injection of inhalation anesthetics also leads to antinociceptive effects in mice [2,10,12]. According to pharmacokinetics and our pilot experiments, we confirmed that i.p.…”

Section: Discussionsupporting

confidence: 92%

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Intrathecal l-arginine reduces the antinociception of sevoflurane in formalin-induced pain in rats

Yin-ming

Ti-jun

Tang

2015

Neuroscience Letters

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The purpose of this study was to investigate the contribution of spinal nitric oxide (NO) to the antinociceptive effects of emulsified sevoflurane in rats. Formalin tests were used to assess the nociceptive response. Immunohistochemistry was performed to determine the effects of emulsified sevoflurane on formalin-induced changes of Fos-like immunoreactive (Fos-LI)-positive neurons in the spinal cord. We found that emulsified sevoflurane administered intraperitoneally at a dosage of 2.5 ml/kg did not impair motor performance in rats, but it significantly decreased the formalin-induced paw licking time. Furthermore, Fos-LI-positive neurons were mainly found in the ipsilateral dorsal horn after the injection of formalin. The administration of emulsified sevoflurane significantly decreased Fos-LI-labeled neurons. Finally, intrathecal L-arginine alone did not affect the basal pain threshold, but it significantly decreased the antinociceptive response of emulsified sevoflurane against formalin injection and the suppressive effects of sevoflurane on formalin-induced Fos protein expression (P<0.05). These data suggest that spinal cord NO is involved in the antinociception of sevoflurane in rats.

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Section: Discussionsupporting

confidence: 92%

“…Many studies have shown that sevoflurane has analgesic properties and that its analgesic properties are mediated by the spinal cord [1,2]. However, the exact mechanisms and pathways of its anesthetic action are not clearly understood.…”

Section: Introductionmentioning

confidence: 98%

Intrathecal l-arginine reduces the antinociception of sevoflurane in formalin-induced pain in rats

Yin-ming

Ti-jun

Tang

2015

Neuroscience Letters

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“…Previous in vivo experiments have shown that GABAA receptors are involved with spinally mediated antinociception in rats, and BIC was used in those experiments to suppress the antinociceptive effects of midazolam (GABA agonist) [28]. It is well known that GABAA receptors exist in the spinal cord and, when activated, cause decreased excitability of the neurons in which they exist [29]. This study provides evidence that the antinociceptive effect of OSR results from the activation of the GABAA receptor.…”

mentioning

confidence: 61%

Oxysophoridine through Intrathecal Injection Induces Antinociception and Increases the Expression of the GABAAα1 Receptor in the Spinal Cord of Mice

Yang

Gao²,

Jia

et al. 2012

Planta Med

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Our researches in recent years have shown that oxysophoridine (OSR), an alkaloid extracted from Siphocampylus verticillatus, presents antinociception through systemic and intracerebroventricular (icv) administration, and that OSR can also increase the GABA-immunopositive cells number in the central nervous system in rats. The purpose of the present study was to investigate the antinociception produced by OSR administered spinally, the interaction between OSR and GABAA receptor (GABAAR) agonists or antagonists on acute thermal nociceptive models (tail-flick test), and the possible alterations on the mRNA and protein expression of GABAAα1 receptors in the spinal cord. ICR mice were tested for their tail withdrawal response to thermal stimulation (tail-flick test). Quantitative real-time PCR and Western blot were used to inspect the influence of OSR administered by intrathecal injection (i. t.) on mRNA and protein expression of the GABAAα1 receptor in mice spinal cord by the formalin test. The experiments showed that OSR (0.13-0.25 mg/site, i. t.) significantly increased the tail withdrawal threshold with a peak effect of 68.35 % MPE at 20 min (p < 0.01). When OSR (0.06 mg/site, i. t.) was administered with gamma aminobutyric acid (GABA) (30.0 µg/site, icv) or muscimol (MUS) (0.10 µg/site, icv), the value of tail-flick latency was remarkably larger than with OSR alone (at 10 min, 45.67 % or 31.45 %, p < 0.01). Picrotoxin (PTX) and bicuculine (BIC) can significantly antagonize the antinociception of OSR. OSR (0.25 mg/site, i. t.) can increase the expression of mRNA and protein of the GABAAα1 receptor in the spinal cord (L5-L6). The results reveal that i. t. administered OSR presents effective antinociception whose action is mainly located in the spinal cord. The antinociception of OSR results from the activation of the GABAA receptor and from the regulation of the GABAAα1 receptor-mediated neurotransmission.

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“…Anesthetic‐sensitive ion‐channel proteins that control neuronal excitability are the most likely channels that an anesthetic acts on. Research on these proteins has revealed that γ‐aminobutyric acid type A (GABA A ) receptors are likely involved in the molecular actions of sevoflurane (11–17). It may also act by inhibiting excitatory ion channels such as neuronal nicotinic and glutamate receptors (12, 18–22).…”

mentioning

confidence: 99%

Anesthetic effects on regional CBF, BOLD, and the coupling between task‐induced changes in CBF and BOLD: An fMRI study in normal human subjects

Qiu

Ramani

Swetye

et al. 2008

Magnetic Resonance in Med

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Functional MR imaging was performed in sixteen healthy human subjects measuring both regional cerebral blood flow (CBF) and blood oxygen level dependent (BOLD) signal when visual and auditory stimuli were presented to subjects in the presence or absence of anesthesia. During anesthesia, 0.25 mean alveolar concentration (MAC) sevoflurane was administrated. We found that low-dose sevoflurane decreased the taskinduced changes in both BOLD and CBF. Within the visual and auditory regions of interest inspected, both baseline CBF and the task-induced changes in CBF decreased significantly during anesthesia. Low-dose sevoflurane significantly altered the task-induced CBF-BOLD coupling; for a unit change of CBF, a larger change in BOLD was observed in the anesthesia condition than in the anesthesia-free condition. Low-dose sevoflurane was also found to have significant impact on the spatial nonuniformity of the task-induced coupling.

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Involvement of GABA and opioid peptide receptors in sevoflurane-induced antinociception in rat spinal cord

Cited by 15 publications

References 17 publications

Intrathecal l-arginine reduces the antinociception of sevoflurane in formalin-induced pain in rats

Intrathecal l-arginine reduces the antinociception of sevoflurane in formalin-induced pain in rats

Oxysophoridine through Intrathecal Injection Induces Antinociception and Increases the Expression of the GABAAα1 Receptor in the Spinal Cord of Mice

Anesthetic effects on regional CBF, BOLD, and the coupling between task‐induced changes in CBF and BOLD: An fMRI study in normal human subjects

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