Involvement of ganglionic cholinergic receptors on the steroidogenesis in the luteal phase in rat

Orozco, Adriana Soledad Vega; Sosa, Zulema; Delgado, Silvia Marcela; Casais, Marilina; Rastrilla, Ana María

doi:10.1016/j.jsbmb.2010.03.040

Cited by 11 publications

(10 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It must be noted that in previous work, we demonstrated in the same SMG-ONP-ovary system that, besides catecholamines, other neurotransmitters such as NO may be released from the ONP by way of the ganglionic stimulus (Vega Orozco et al 2010). Evidence has been reported that supports the involvement of NO in ovarian physiology (Fridén et al 2000, Motta et al 2001).…”

Section: Discussionsupporting

confidence: 53%

“…Given the importance of the neural regulation from the peripheral nervous system on ovarian physiology (Sosa et al 2000, Casais et al 2006, Vega Orozco et al 2010 and taking into account that E 2 acts on peripheral organs and on the plasticity of sympathetic neurons associated with reproduction (Anesetti et al 2009), the general aim of this work was to examine the distributions of ERa and ERb in neurons of the SMG and to evaluate the importance of these receptors in ovarian steroidogenesis and their possible relation with NO release.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Involvement of the oestrogenic receptors in superior mesenteric ganglion on the ovarian steroidogenesis in rat

Orozco¹,

Daneri²,

Anesetti³

et al. 2012

Reproduction

Self Cite

View full text Add to dashboard Cite

Oestradiol (E 2 ) is a key hormone in the regulation of reproductive processes. The aims of this work were a) to examine the distributions of oestrogen receptor a (ERa) and ERb in the neurons of the superior mesenteric ganglion (SMG) in the oestrus stage by immunohistochemistry, b) to demonstrate whether E 2 in the SMG modifies progesterone (P 4 ), androstenedione (A 2 ) and nitrite release in the ovarian compartment on oestrus day and c) to demonstrate whether E 2 in the ganglion modifies the activity and gene expression in the ovary of the steroidogenic enzymes 3b-hydroxysteroid dehydrogenase (3b-HSD) and 20a-hydroxysteroid dehydrogenase (20a-HSD). The ex vivo SMG-ovarian nervous plexus-ovary system was used. E 2 , tamoxifen (Txf) and E 2 plus Txf were added in the ganglion to measure ovarian P 4 release, while E 2 alone was added to measure ovarian A 2 and nitrites release. Immunohistochemistry revealed cytoplasmic ERa immunoreactivity only in the neural somas in the SMG. E 2 increased ovarian P 4 and A 2 release at 15, 30 and 60 min but decreased nitrites. The activity and gene expression of 3b-HSD increased, while the activity and gene expression of 20a-HSD did not show changes with respect to the control. Txf in the ganglion diminished P 4 release only at 60 min. E 2 plus Txf in the ganglion reverted the effect of E 2 alone and the inhibitory effect of Txf. The results of this study demonstrate that ERa activation in the SMG has an impact on ovarian steroidogenesis in rats, thus providing evidence for the critical role of peripheral system neurons in the control of ovarian functions under normal and pathological conditions.

show abstract

Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Involvement of the oestrogenic receptors in superior mesenteric ganglion on the ovarian steroidogenesis in rat

Orozco¹,

Daneri²,

Anesetti³

et al. 2012

Reproduction

Self Cite

View full text Add to dashboard Cite

show abstract

“…This system has been previously characterised by Vega Orozco et al . The procedure was carried out 5‐6 minutes after the i.c.v treatment injection, taking into account previous descriptions of the anatomical trajectory . The rats of the different experimental groups were anaesthetised and the SMG‐ONP‐Ovary system was immediately removed by dissection.…”

Section: Methodsmentioning

confidence: 99%

“…Both plexuses are mainly formed by sympathetic postganglionic fibres for which neuronal somas are located in the superior mesenteric ganglion (SMG) and the coeliac ganglion, respectively . Thus, cholinergic neural stimulation of SMG causes changes in ovarian P 4 release and 3β‐HSD expression, depending on the oestrous cycle stage . However, the modulatory action of neurosteroids on the 3β‐HSD enzyme activity and gene expression modulated by the PNS has not yet been determined.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

“Rapid actions of the neurosteroid allopregnanolone on ovarian and hypothalamic steroidogenesis: Central and peripheral modulation”

Cáceres

Orozco

Cabrera

et al. 2020

J Neuroendocrinology

View full text Add to dashboard Cite

The present study aimed to determine whether an i.c.v. administration of allopregnanolone (ALLO) rapidly modifies the hypothalamic and ovarian 3β‐hydroxysteroid dehydrogenase (3β‐HSD) enzymatic activity and gene expression in in vivo and ex vivo systems in pro‐oestrus (PE) and dioestrus I (DI) rats. Animals were injected with vehicle, ALLO, bicuculline or bicuculline plus ALLO and were then killed. In the in vivo experiment, the hypothalamus, ovaries and serum were extracted and analysed. In the ex vivo experiment, the superior mesenteric ganglion ‐ ovarian nerve plexus ‐ ovary system was extracted and incubated during 120 minutes at 37 ºC. The serum and ovarian compartment fluids were used to determine progesterone by radioimmunoanalysis. In the in vivo experiments, ALLO caused a decrease in hypothalamic and ovarian 3β‐HSD enzymatic activity during PE. During DI, ALLO increased hypothalamic and ovarian 3β‐HSD activity and gene expression. The ovarian 3β‐HSD activity increased in both stages in the ex vivo system; gene expression increased only during DI. ALLO induced an increase in serum progesterone only in D1 and in the ovarian incubation liquids in both stages. All findings were reversed by an injection of bicuculline before ALLO. Ovarian steroidogenic changes could be attributed to signals coming from ganglion neurones, which are affected by the acute central neurosteroid stimulation. The i.c.v. administration of ALLO via the GABAergic system altered 3β‐HSD activity and gene expression, modulating the neuroendocrine axis. The present study reveals the action that ALLO exerts on the GABAA receptor in both the central and peripheral nervous system and its relationship with hormonal variations. ALLO is involved in the “fine tuning” of neurosecretory functions as a potent modulator of reproductive processes in female rats.

show abstract

Long-term estradiol-17β exposure decreases the cholinergic innervation pattern of the pig ovary

Jana

Meller

Czajkowska

et al. 2018

Annals of Anatomy - Anatomischer Anzeiger

View full text Add to dashboard Cite

Involvement of ganglionic cholinergic receptors on the steroidogenesis in the luteal phase in rat

Cited by 11 publications

References 47 publications

Involvement of the oestrogenic receptors in superior mesenteric ganglion on the ovarian steroidogenesis in rat

Involvement of the oestrogenic receptors in superior mesenteric ganglion on the ovarian steroidogenesis in rat

“Rapid actions of the neurosteroid allopregnanolone on ovarian and hypothalamic steroidogenesis: Central and peripheral modulation”

Long-term estradiol-17β exposure decreases the cholinergic innervation pattern of the pig ovary

Contact Info

Product

Resources

About