Involvement of gap junctional communication in myogenesis

Constantin, Bruno; Cronier, Laurent

doi:10.1016/s0074-7696(00)96001-7

Cited by 47 publications

(47 citation statements)

References 294 publications

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“…Although a role for gap junctions during myogenesis had been previously suggested (reviewed by Constantin and Cronier, 2000) their functional importance during regeneration of skeletal muscle was hitherto largely unknown. Furthermore, a detailed analysis of the expression of connexin isoforms in different muscle cell types had yet to be carried out.…”

Section: Discussionmentioning

confidence: 99%

“…The possible role of gap junctional communication in myogenesis has been previously studied (Constantin and Cronier, 2000). Ultrastructural analyses in developing rat and chicken skeletal muscle demonstrated the presence of gap junctions between myoblasts and between myoblasts and myotubes (Rash and Staehelin, 1974;Kalderon et al, 1977;Duxson et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, Cx43 is expressed in vitro in prefusional myoblasts of the C 2 C 12 cell line (Constantin and Cronier, 2000). Myogenin mRNA expression and myotube formation during myoblast differentiation in the L6 cell line is reversibly blocked by 18-β-glycyrrhetinic acid or octanol, which inhibit gap junction channels (Proulx et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

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Expression of connexins during differentiation and regeneration of skeletal muscle: functional relevance of connexin43

Araya

Eckardt

Maxeiner

et al. 2005

Journal of Cell Science

100

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The molecular mechanisms regulating skeletal muscle regeneration and differentiation are not well understood. We analyzed the expression of connexins (Cxs) 40, 43 and 45 in normal and regenerating tibialis anterior muscle and in primary cultures of differentiating myoblasts in adult and newborn mice, respectively. Cxs 45 and 43, but not 40, were strongly expressed in normal muscle and their expression was upregulated during regeneration. Furthermore, the functional role of Cx43 during differentiation and regeneration was examined after induced deletion of Cx43 in transgenic mice. In vivo, the inducible deletion of Cx43 delayed the formation of myofibers and prolonged the expression of myogenin during regeneration. In primary cultures of satellite cell-derived myoblasts, induced deletion of Cx43 led to decreased expression of myogenin and MyoD, dye coupling, creatine kinase activity and myoblast fusion. Thus, the expression of Cx45 and Cx43 is upregulated during skeletal muscle regeneration and Cx43 is required for normal myogenesis in vitro and adult muscle regeneration in vivo.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression of connexins during differentiation and regeneration of skeletal muscle: functional relevance of connexin43

Araya

Eckardt

Maxeiner

et al. 2005

Journal of Cell Science

100

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“…Gap junctions are known to mediate important effects during skeletal myogenesis 14 ; hence, it is plausible that the composition of ion channels subserving membrane depolarization may be altered, with a resultant reduction in excitability. Microelectrode studies with measurements of action potential (AP) and of membrane currents such as I Na may offer mechanistic insights.…”

Section: Discussionmentioning

confidence: 99%

Fibroblasts Can Be Genetically Modified to Produce Excitable Cells Capable of Electrical Coupling

et al. 2005

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Background-Cardiac conduction occurs in an electrical syncytium of excitable cells connected by gap junctions.Disruption of these electrophysiological properties causes conduction slowing or block. Depending on the location of affected cells within the heart, this has the potential to result in clinical syndromes such as atrioventricular block. With a view to developing gene therapy strategies for repairing cardiac conduction defects, we sought to establish whether the phenotype of fibroblasts can be modified by gene transfer to produce cells capable of electrical excitation and coupling. Methods and Results-High-titer lentiviral vectors encoding MyoD, a myogenic transcription factor, and connexin43, a gap junction protein, were produced by established methods. Human dermal fibroblasts (HDFs) were efficiently (Ͼ80%) transduced at a multiplicity of infection of 50. HDFs transduced with the MyoD-encoding vector underwent myogenic conversion, as evidenced by myotube formation and detection of muscle-specific proteins. Importantly, calcium transients indicative of membrane excitability were observed in MyoD-induced myotubes after loading with a calcium-sensitive dye and electrical stimulation.

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“…Ultrastructural analysis demonstrate gap junction-like structures between interacting myoblasts, and between myoblasts and myotubes in developing muscles of the rat (Kalderon et al, 1977;Ling et al, 1992) but the expression of only Cx40 and Cx43 has been reported in subcompartments of developing muscles in mouse and rat (Constantin and Cronier, 2000).…”

Section: Introductionmentioning

confidence: 99%

The novel mouseconnexin39gene is expressed in developing striated muscle fibers

Maltzahn

Euwens

Willecke

et al. 2004

Journal of Cell Science

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The recently identified mouse connexin39 (mCx39) gene encodes a peptide of 364 amino acids that shows only 61% sequence similarity to its putative human orthologue connexin40.1 (hCx40.1). The coding regions of mCx39 and hCx40.1 are located on two different exons as described for murine and human connexin36. Northern blot and RT-PCR analyses revealed that mCx39 is expressed after embryonic day (ED) 13.5 up to birth and is absent from the adult stage. Polyclonal antibodies raised to a peptide corresponding to the 16 C-terminal amino acid residues detected a protein band of about 40 kDa apparent molecular mass in lysates of several embryonic tissues. In sections of ED14.5, ED16.5 and neonatal (P0) tissues, immunofluorescent signals were prominent between myotubes in the developing diaphragm, within the intercostal muscle, in the region around the occipital bone, as well as in muscles of the limb, tongue and connective tissue around the eye. These antibodies yielded punctate signals on apposed plasma membranes of HeLa cells transfected with Cx39 cDNA but did not react with wild-type cells. Furthermore, no intercellular permeation of microinjected neurobiotin and other tracers could be detected in Cx39 transfected HeLa cells. However, after microinjection of Alexa488 into myotubes of dissected neonatal diaphragm, we found spreading of this dye into neighbouring cells. As expression of no other known connexin could be verified in these cells, intercellular dye transfer might result from functional expression of Cx39 in developing striated muscle fibers.

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Involvement of gap junctional communication in myogenesis

Cited by 47 publications

References 294 publications

Expression of connexins during differentiation and regeneration of skeletal muscle: functional relevance of connexin43

Expression of connexins during differentiation and regeneration of skeletal muscle: functional relevance of connexin43

Fibroblasts Can Be Genetically Modified to Produce Excitable Cells Capable of Electrical Coupling

The novel mouseconnexin39gene is expressed in developing striated muscle fibers

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