Involvement of GTP-binding protein in the induction of phytoalexin biosynthesis in cultured carrot cells

Kurosaki, Fumiya; Yamashita, Aoi; Arisawa, Munehisa

doi:10.1016/s0168-9452(01)00407-1

Cited by 21 publications

(13 citation statements)

References 21 publications

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“…Early pharmacological data suggested a less-defined role of heterotrimeric G proteins in the generation of reactive oxygen by plasma membrane redox processes (in Beta vulgaris [Vera-Estrella et al, 1994] and in Glycine max [Rajasekhar et al, 1999]) and the overproduction of phytoalexins (in Eschscholzia californica [Roos et al, 1999] and in Daucus carota [Kurosaki et al, 2001]). By contrast, Ga-deficient rice (Oryza sativa) mutants were not impaired in the generation of reactive oxygen, medium alkalinization, and expression of early genes of phytoalexin production (Tsukada et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

The Gα Protein Controls a pH-Dependent Signal Path to the Induction of Phytoalexin Biosynthesis in Eschscholzia californica

et al. 2006

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Section: Introductionmentioning

confidence: 99%

The Gα Protein Controls a pH-Dependent Signal Path to the Induction of Phytoalexin Biosynthesis in Eschscholzia californica

et al. 2006

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“…The reduction of the transcriptional level of Dcga in carrot seedlings might be, possibly, one of the physiological events in down-regulation of cellular processes against these stresses though no direct evidence is available at present. We reported previously 11) that GTP-binding protein functions in the induction of defense-related secondary metabolism in carrot cells, how-ever, as shown in Fig. 4, expression level of Dcga did not respond to the treatment of carrot with oligogalacturonides.…”

Section: Resultsmentioning

confidence: 57%

“…16) Since carrot gene(s) encoding either Gb or Gg subunit has been isolated not yet, there is no guarantee that Dcga product functions in the cells as a component of heterotrimeric complex. However, biochemical responses of carrot toward several reagents, which should perturb the activities of heterotrimeric GTP-binding proteins, 11) suggest the possibility that, as is in animal cells and several model plants, Dcga product A cDNA clone encoding a subunit of GTP-binding protein, Dcga, was isolated from carrot seedlings, and the deduced amino acid sequences was compared with the proteins from various biological sources (Arab, Arabidopsis thaliana; Rice, Oryza sativa; Go, Gi, Gz, Gt and Gs are a subunit proteins from human). Their GenBank accession numbers were shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…[8][9][10] We have previously shown that GTP-binding protein(s) mediates an inducible plant defense response, phytoalexin biosynthesis, in carrot cells triggered by elicitor-active chemicals. 11) In order to understand the properties and functions of a subunit of GTP-binding protein in the induced plant defense response, in the present experiments, we attempted to isolate cDNA that encodes a subunit of the mediator protein from carrot. The properties of the isolated Ga gene, such as transcriptional regulation and genomic organization, were also examined.…”

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confidence: 99%

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Cloning and Expression of Dcga Gene Encoding .ALPHA. Subunit of GTP-Binding Protein in Carrot Seedlings

Asakura

Kurosaki

2007

Biological & Pharmaceutical Bulletin

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Transmembrane signaling mediated by heterotrimeric GTP-binding proteins is one of the most conservative mechanisms in eukaryote cells, and these G proteins transmit the numerous external stimuli to the activation of appropriate intracellular functional proteins called effectors. 1,2) It has been well known [1][2][3] that G proteins are consisted of three subunits, a, b, g, and a subunit is dissociated from the heterotrimeric complex upon the activation by G proteincoupled receptor. The dissociation of a subunit results in the activation of several functional proteins called effector, including adenylyl cyclase, guanylyl cyclase, phosphodiesterase and phospholipase, to evoke various cellular responses. Multiple a subunits of GTP-binding proteins have been reported from mammalian cells, and these Ga subunits are appropriately classified into several subfamilies such as Gs, Gi, Gq and Go according to their target effector proteins and cell physiological functions.3)It has been demonstrated that GTP-binding proteins of higher plants also play important roles in various cellular processes including blue or red light-mediated responses, 4) plant hormone signaling, 5) regulation of ion channels 6) and pathogen-induced resistance mechanisms. 7) However, only very limited information is available about the properties of G proteins and their target effector molecules in plant cells. Several cDNAs encoding Ga subunit have been isolated from some 'model plants' such as rice, 8) potato, 9) Arabidopsis thaliana, 10) however, unlike in mammalians, the possibility has been suggested that plant G protein a subunits might be encoded by very small numbers of genes. [8][9][10] We have previously shown that GTP-binding protein(s) mediates an inducible plant defense response, phytoalexin biosynthesis, in carrot cells triggered by elicitor-active chemicals.11) In order to understand the properties and functions of a subunit of GTP-binding protein in the induced plant defense response, in the present experiments, we attempted to isolate cDNA that encodes a subunit of the mediator protein from carrot. The properties of the isolated Ga gene, such as transcriptional regulation and genomic organization, were also examined. MATERIALS AND METHODSDcga Genes from Carrot Carrot seedlings were grown in a green house of Experimental Station for Medicinal Plant Research in University of Toyama, and total RNA was isolated from the plant with RNeasy Plant Mini Kit (Qiagen). The RNA obtained (approximately 1 mg) was subjected to the rapid amplification of cDNA end (RACE) methods using the GeneRacer Kit (Invitrogen) after the generation of cDNA templates by reverse-transcription (RT) reaction with AMV-RT. The 3Ј-RACE was performed with GeneRacer Oligo dT as the reverse primer, and 5Ј-TTY ACN GTN TGG GAY GTN GGN GGN CAR GA-3Ј, 5Ј-GCC AGG ACA AGA TCC GTC CTT T-3Ј and 5Ј-GGG TAA TGA ACT CCA AGT TCC TGA CTG C-3Ј as the gene-specific forward primers for polymerase chain reaction (PCR) amplification of the DNA fragments. The 5Ј-RACE was carried out with Gen...

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“…Biochemical and genetic evidences obtained from studies on G protein activators or inhibitors shows that heteromeric and monomeric subunits of G proteins have undisputable contribution in transmitting signals to molecules, including GTPase, ion channels, phospholipases and several signaling pathways that are effectors in the responses to various biotic and abiotic stress conditions including pathogen elicitation, O 3 treatment, and drought etc. [22][23][24]. Supplementation of G protein inhibitors such as suramin in the ambience of cell inhibits the activity of receptor-coupled G proteins and consequently the phytoalexin production suggesting the possible involvement of G protein is signal communication within the cell [25].…”

Section: Elicitor Signaling System Inside the Cellmentioning

confidence: 99%

Elicitor-Induced Cellular and Molecular Events Are Responsible for Productivity Enhancement in Hairy Root Cultures: An Insight Study

Goel

Mehrotra

Kukreja

2011

Appl Biochem Biotechnol

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A wide range of external stress stimuli triggers a plant cell to undergo a complex network of reactions that ultimately lead to the synthesis and accumulation of secondary metabolites. These secondary metabolites help the plant to survive under stress challenge. The potential of biotic and abiotic elicitors for the induction and enhancement of secondary metabolite production in various culture systems including hairy root (HR) cultures is well-known. The elicitor-induced defense responses involves signal perception of elicitor by a cell surface receptor followed by its transduction involving some major cellular and molecular events including activation of major secondary message signaling pathways. This result in induction of gene expressions escorting to the synthesis of various proteins mainly associated with plant defense responses and secondary metabolite synthesis and accumulation. The review discusses the elicitor-induced various cellular and molecular events and correlates them with enhanced secondary metabolite synthesis in HR systems. Further, this review also concludes that combining elicitation with in-silico approaches enhances the usefulness of this practice in better understanding and identifying the rate-limiting steps of biosynthetic pathways existing in HRs which in turn can contribute towards better productivity by utilizing metabolic engineering aspects.

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Involvement of GTP-binding protein in the induction of phytoalexin biosynthesis in cultured carrot cells

Cited by 21 publications

References 21 publications

The Gα Protein Controls a pH-Dependent Signal Path to the Induction of Phytoalexin Biosynthesis in Eschscholzia californica

The Gα Protein Controls a pH-Dependent Signal Path to the Induction of Phytoalexin Biosynthesis in Eschscholzia californica

Cloning and Expression of Dcga Gene Encoding .ALPHA. Subunit of GTP-Binding Protein in Carrot Seedlings

Elicitor-Induced Cellular and Molecular Events Are Responsible for Productivity Enhancement in Hairy Root Cultures: An Insight Study

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