Involvement of HVEM receptor in activation of nuclear factor κB by herpes simplex virus 1 glycoprotein D

The Virion Host Shutoff RNase Plays a Key Role in Blocking the Activation of Protein Kinase R in Cells Infected with Herpes Simplex Virus 1

“…Infected cells were subjected to cytoplasmic protein extraction for Western blot analysis (24). Cell pellets containing approximately 3 ϫ 10 6 cells were collected at the time indicated for each experiment, washed in 1ϫ phosphate-buffered saline (PBS), and resuspended in 150 l of hypotonic buffer A (10 mM HEPES, pH 7.9, 1.5 mM MgCl 2 , 10 mM KCl, 0.5 mM dithiothreitol [DTT], 0.2 mM phenylmethylsulfonyl fluoride [PMSF]).…”

Protection against HPV-16–Associated Tumors Requires the Activation of CD8+ Effector Memory T Cells and the Control of Myeloid-Derived Suppressor Cells

“…The HSV-1 gD protein, expressed by the vaccine formulation studied here, was shown to exert CD8 þ T-cell activation through binding to HVEM (41). The gD/HVEM interaction induces NF-kB activation and blockade of CD160 and BTLA (B and T lymphocyte attenuator) coinhibitory signals, contributing to the induction of T-cell effector responses (39,40,42 (46). In the mouse tumor model used in this study, tumor progression induced the accumulation of MDSCs, which have been shown to reduce CD8 þ T-cell activity and, thus, can be detrimental to eradication of tumors at more advanced stages.…”

ERK1-Based Pathway as a New Selective Mechanism To Modulate CCR5 with Natural Antibodies

“…The extracts were prepared as previously described (13). Briefly, 3 3 10 6 cells were collected, washed in PBS 13, and resuspended in hypotonic buffer A (10 mM HEPES, pH 7.9; 1.5 mM MgCl 2 ; 10 mM KCl; 0.5 mM DTT; 0.2 mM PMFS).…”