Involvement of hyaluronan synthesis in ovarian follicle growth in rats

Takahashi, Noriyuki; Tarumi, Wataru; Ishizuka, Bunpei

doi:10.1530/rep-13-0464

Cited by 22 publications

(15 citation statements)

References 33 publications

(46 reference statements)

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“…In particular, we focused on hyaluronan as its metabolism plays a central role in follicular development (Figure ). It is synthetized in granulosa cells and secreted into the follicular fluid where it maintains folliculogenesis and prevents oocyte degeneration . Hyaluronan degradation may be associated with follicular atresia .…”

Section: Extra‐cerebral Involvementmentioning

confidence: 99%

Vanishing white matter: a leukodystrophy due to astrocytic dysfunction

et al. 2018

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VWM is one of the most prevalent leukodystrophies with unique clinical, pathological and molecular features. It mostly affects children, but may develop at all ages, from birth to senescence. It is dominated by cerebellar ataxia and susceptible to stresses that act as factors provoking disease onset or episodes of rapid neurological deterioration possibly leading to death. VWM is caused by mutations in any of the genes encoding the five subunits of the eukaryotic translation initiation factor 2B (eIF2B). Although eIF2B is ubiquitously expressed, VWM primarily manifests as a leukodystrophy with increasing white matter rarefaction and cystic degeneration, meager astrogliosis with no glial scarring and dysmorphic immature astrocytes and increased numbers of oligodendrocyte progenitor cells that are restrained from maturing into myelin-forming cells. Recent findings point to a central role for astrocytes in driving the brain pathology, with secondary effects on both oligodendroglia and axons. In this, VWM belongs to the growing group of astrocytopathies, in which loss of essential astrocytic functions and gain of detrimental functions drive degeneration of the white matter. Additional disease mechanisms include activation of the unfolded protein response with constitutive predisposition to cellular stress, failure of astrocyte-microglia crosstalk and possibly secondary effects on the oxidative phosphorylation. VWM involves a translation initiation factor. The group of leukodystrophies due to defects in mRNA translation is also growing, suggesting that this may be a common disease mechanism. The combination of all these features makes VWM an intriguing natural model to understand the biology and pathology of the white matter.

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Section: Extra‐cerebral Involvementmentioning

confidence: 99%

Vanishing white matter: a leukodystrophy due to astrocytic dysfunction

et al. 2018

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“…In the ovary, HA inhibits human follicular granulosa cell (GC) apoptosis 3 , consistent with its function in porcine GC (pGC) apoptosis 4 . Inhibition of ovarian HA synthesis with chemicals, such as deoxynivalenol, induces follicular atresia, and addition of Streptomyces hyaluronidase, which specifically degrades HA, induces the arrest of follicle growth in an in vitro culture system 5 . Likewise, the anti-apoptotic role of HA is evident in other cells, such as skin fibroblasts 6 and smooth muscle cells 7 .…”

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confidence: 99%

“…Knockout studies in mice showed that Has2 −/− embryos lack HA, resulting in embryonic lethality during mid-gestation 10 11 . HAS2 has a close relationship with ovary development and function, and is predominantly located in the GC layers of rat and sheep ovaries 5 12 . Knockdown of CCAAT-enhancer-binding protein CEBβ decreases the HAS2 mRNA level and results in the arrest of pGCs at S phase of the cell cycle, indicating that HAS2 plays an important role in GC function 13 .…”

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confidence: 99%

Conserved miR-26b enhances ovarian granulosa cell apoptosis through HAS2-HA-CD44-Caspase-3 pathway by targeting HAS2

Liu

Wang

et al. 2016

Sci Rep

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The hyaluronan synthase 2 (HAS2)-hyaluronic acid (HA)-CD44-Caspase-3 pathway is involved in ovarian granulosa cell (GC) functions in mammals. HAS2 is a key enzyme required for HA synthesis and is the key factor in this pathway. However, the regulation of HAS2 and the HAS2-mediated pathway by microRNAs in GCs is poorly understood. Here, we report that miR-26b regulates porcine GC (pGC) apoptosis through the HAS2-HA-CD44-Caspase-3 pathway by binding directly to the 3′- untranslated region of HAS2 mRNA. Knockdown of miR-26b reduced pGC apoptosis. Luciferase reporter assays demonstrated that HAS2 is a direct target of miR-26b in pGCs. Knockdown and overexpression of miR-26b increased and decreased, respectively, HA content, and HAS2 and CD44 expression in pGCs. At the same time, inhibition and overexpression of miR-26b decreased and increased the expression of Caspase-3, a downstream factor in the HAS2-HA-CD44 pathway. Moreover, knockdown of HAS2 enhanced pGC apoptosis, reduced the inhibitory effects of a miR-26b inhibitor on pGC apoptosis, repressed HA content and CD44 expression, and promoted Caspase-3 expression. In addition, overexpression of HAS2 has a opposite effect. Collectively, miR-26b positively regulates pGC apoptosis via a novel HAS2-HA-CD44-Caspase-3 pathway by targeting the HAS2 gene.

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“…In cow, but also in horse in particular the expression of HAS2 is strongly induced by gonadotropins in GC of pre-ovulatory follicles [ 39 , 40 ]. In the rat Has1 and Has2 transcripts are expressed in cells of the theca and granulosa, whereas HA molecules are predominantly localized in the extracellular matrix of the theca layer, within the follicular fluid, but also the zona pellucida of large antral follicles [ 41 ]. Interestingly, also B3GALT2 transcripts, encoding the membrane-bound glycoprotein enzyme “UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase”, which is involved in the modification of cell surface molecules and thus cell-cell interactions were strongly up-regulated in GC of the HS group.…”

Section: Discussionmentioning

confidence: 99%

Exposure of Lactating Dairy Cows to Acute Pre-Ovulatory Heat Stress Affects Granulosa Cell-Specific Gene Expression Profiles in Dominant Follicles

et al. 2016

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High environmental temperatures induce detrimental effects on various reproductive processes in cattle. According to the predicted global warming the number of days with unfavorable ambient temperatures will further increase. The objective of this study was to investigate effects of acute heat stress during the late pre-ovulatory phase on morphological, physiological and molecular parameters of dominant follicles in cycling cows during lactation. Eight German Holstein cows in established lactation were exposed to heat stress (28°C) or thermoneutral conditions (15°C) with pair-feeding for four days. After hormonal heat induction growth of the respective dominant follicles was monitored by ultrasonography for two days, then an ovulatory GnRH dose was given and follicular steroid hormones and granulosa cell-specific gene expression profiles were determined 23 hrs thereafter. The data showed that the pre-ovulatory growth of dominant follicles and the estradiol, but not the progesterone concentrations tended to be slightly affected. mRNA microarray and hierarchical cluster analysis revealed distinct expression profiles in granulosa cells derived from heat stressed compared to pair-fed animals. Among the 255 affected genes heatstress-, stress- or apoptosis associated genes were not present. But instead, we found up-regulation of genes essentially involved in G-protein coupled signaling pathways, extracellular matrix composition, and several members of the solute carrier family as well as up-regulation of FST encoding follistatin. In summary, the data of the present study show that acute pre-ovulatory heat stress can specifically alter gene expression profiles in granulosa cells, however without inducing stress related genes and pathways and suggestively can impair follicular growth due to affecting the activin-inhibin-follistatin system.

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Involvement of hyaluronan synthesis in ovarian follicle growth in rats

Cited by 22 publications

References 33 publications

Vanishing white matter: a leukodystrophy due to astrocytic dysfunction

Vanishing white matter: a leukodystrophy due to astrocytic dysfunction

Conserved miR-26b enhances ovarian granulosa cell apoptosis through HAS2-HA-CD44-Caspase-3 pathway by targeting HAS2

Exposure of Lactating Dairy Cows to Acute Pre-Ovulatory Heat Stress Affects Granulosa Cell-Specific Gene Expression Profiles in Dominant Follicles

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