Involvement of Hydrogen Peroxide Generated by Polyamine Oxidative Degradation in the Development of Lateral Roots in Soybean

Abstract

In order to determine whether hydrogen peroxide (H 2 O 2 ) generated by polyamine oxidative degradation is involved in the development of lateral roots in soybean, the length and the number of lateral roots, the activities of polyamine oxidases and diamine oxidases, and the endogenous free polyamine and H 2 O 2 content were analyzed in soybean (Glycine max (Linn.) Merr.) main roots of 2-d-old seedlings after treatments for 2 d with exogenous β β β β β-hydroxyethylhydrazine (an inhibitor of polyamine oxidases), H 2 O 2 , putrescine, cyclohexylamine (an inhibitor of spermidine synthase) or N,N'-dimethylthiourea (a scavenger of hydrogen peroxide). β β β β β-hydroxyethylhydrazine treatment strongly inhibited the development of lateral roots in soybean seedlings, reduced the activities of polyamine oxidases and diamine oxidases, decreased H 2 O 2 levels, and led to the accumulation of endogenous polyamines in the main roots. The inhibitory effect of β β β β β-hydroxyethylhydrazine on root development could be alleviated by exogenously applied 10 µmol/L H 2 O 2 (a major product of polyamine oxidation). Treatment with cyclohexylamine and putrescine promoted root growth slightly, but treatment with cyclohexylamine plus N,N'-dimethylthiourea or putrescine plus N,N'-dimethylthiourea prevented the development of soybean lateral roots. The effects of these treatments on the development of soybean lateral roots were consistent with the changes in endogenous H 2 O 2 levels. These results suggest that the development of soybean lateral roots is associated with the oxidative degradation of polyamines, and that their products, especially H 2 O 2 , are likely to play an important role in the growth of soybean lateral roots.

Nitric oxide induced by polyamines involves antioxidant systems against chilling stress in tomato (Lycopersicon esculentum Mill.) seedling

“…PAs can enhance H 2 O 2 production via Put, Spd, or Spm catabolism (Su et al, 2006;Alcázar et al, 2010;Wimalasekera et al, 2011;Moschou et al, 2012;Pál et al, 2015). H 2 O 2 plays a dual role in plants: at low concentrations, it serves as a signal molecule, playing a pivotal role in signal transduction network under various stress conditions (Tanou et al, 2009;Jiang et al, 2012;Lizárraga-Paulín et al, 2013); …”

“…In Zea mays, PAO modulates H 2 O 2 production during wound healing (Angelini et al, 2008). In the development of soybean lateral roots, DAO and PAO play important roles in H 2 O 2 formation (Su et al, 2006).…”

Influence of anti- and prooxidants on rhizogenesis from hypocotyls of Mesembryanthemum crystallinum L. cultured in vitro

“…The signaling role of ROS in root formation was explored in numerous studies (Foreman et al 2003;Rentel et al 2004;Pasternak et al 2005;Su et al 2006;Dunand et al 2007;Li et al 2007;Konieczny et al 2014;Takáč et al Fig. 2).…”

Integrated effect of arbuscular mycorrhizal fungi and hydrogen peroxide on the root system of trifoliate orange seedlings

“…Exogenous H 2 O 2 also mediates the growth of primary root 10,11 , lateral roots, and root hairs 12,13 . The lateral-root formation of soya bean is strongly inhibited by β-hydroxyethyl hydrazine, while the inhibitory effect is reversed after exogenous H 2 O 2 treatment 14 . In mung bean and cucumber, H 2 O 2 could significantly promote the formation and growth of adventitious roots in vivo 15,16 The objective of the present work was to investigate the integrated effects of both AMF and H 2 O 2 on the sugar contents and other traits of RSA of trifoliate orange (Poncirus trifoliata L.…”