Involvement of<i><scp>O</scp>s<scp>P</scp>ht1;4</i>in phosphate acquisition and mobilization facilitates embryo development in rice

Fang, Zhang; Sun, Yuchun; Pei, Wenxia; Jain, Ajay; Sun, Rui; Cao, Yue; Wu, Xueneng; Jiang, Tingting; Zhang, Liang; Fan, Xing; Chen, Aiqun; Shen, Qirong; Xu, Guohua; Sun, Shubin

doi:10.1111/tpj.12804

Cited by 125 publications

(100 citation statements)

References 34 publications

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“…These include the phosphate transporters (PHTs) and PHO-type Pi transporters. The plasma membrane-located PHT1s are responsible for the absorption of phosphate, as has been studied in Arabidopsis and rice (3,4,24,25). For Pi translocation, the Golgi located PHO1 plays an important role (1,16).…”

Section: Discussionmentioning

confidence: 99%

A vacuolar phosphate transporter essential for phosphate homeostasis inArabidopsis

Liu

Yang

Luan

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

184

162

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Inorganic phosphate (Pi) is stored in the vacuole, allowing plants to adapt to variable Pi availability in the soil. The transporters that mediate Pi sequestration into vacuole remain unknown, however. Here we report the functional characterization of Vacuolar Phosphate Transporter 1 (VPT1), an SPX domain protein that transports Pi into the vacuole in Arabidopsis. The vpt1 mutant plants were stunted and consistently retained less Pi than wild type plants, especially when grown in medium containing high levels of Pi. In seedlings, VPT1 was expressed primarily in younger tissues under normal conditions, but was strongly induced by high-Pi conditions in older tissues, suggesting that VPT1 functions in Pi storage in young tissues and in detoxification of high Pi in older tissues. As a result, disruption of VPT1 rendered plants hypersensitive to both low-Pi and high-Pi conditions, reducing the adaptability of plants to changing Pi availability. Patch-clamp analysis of isolated vacuoles showed that the Pi influx current was severely reduced in vpt1 compared with wild type plants. When ectopically expressed in Nicotiana benthamiana mesophyll cells, VPT1 mediates vacuolar influx of anions, including Pi, SO42−, NO3−, Cl−, and malate with Pi as that preferred anion. The VPT1-mediated Pi current amplitude was dependent on cytosolic phosphate concentration. Single-channel analysis showed that the open probability of VPT1 was increased with the increase in transtonoplast potential. We conclude that VPT1 is a transporter responsible for vacuolar Pi storage and is essential for Pi adaptation in Arabidopsis.

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Section: Discussionmentioning

confidence: 99%

A vacuolar phosphate transporter essential for phosphate homeostasis inArabidopsis

Liu

Yang

Luan

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

184

162

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“…Pi transport across the plasma membrane into the cytosol is mediated by members of the PHT1 family of Pi/H+ cotransporters [8]. Although several members of the PHT1 gene family are expressed in rice (Oryza sativa) and wheat (Triticum aestivum) seed tissues [9][10][11], our current understanding of the dynamics and of proteins participating in seed Pi homeostasis is very fragmentary. In particular, it is currently unknown how Pi is exported from the maternal seed coat to the embryo.…”

Section: Introductionmentioning

confidence: 99%

PHO1 Exports Phosphate from the Chalazal Seed Coat to the Embryo in Developing Arabidopsis Seeds

et al. 2017

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Abstract:Seed production requires the transfer of nutrients from the maternal seed coat to the filial endosperm and embryo. Since seed coat and filial tissues are symplasmically isolated, nutrients arriving in the seed coat via the phloem must be exported to the apoplast before reaching the embryo. Proteins implicated in the transfer of inorganic phosphate (Pi) from the seed coat to the embryo are unknown despite seed P content being an important agronomic trait. Here, we show that the Arabidopsis Pi exporter PHO1 and PHOH1 are expressed in the chalazal seed coat (CZSC) of developing seeds. PHO1 is additionally expressed in developing ovules. Phosphorus (P) content and Pi flux between seed coat and embryo was analyzed in seeds from grafts between WT roots and scions from either pho1, phoh1 or pho1 phoh1 double mutant. While P content and distribution between seed coat and embryo in fully mature dry seeds of these mutants are similar to WT, at the mature green stage of seed development the seed coat of the pho1 and pho1 phoh1 mutants, but not of the phoh1 mutant, retains approximately 2-fold more P than its WT control. Expression of PHO1 under a CZSCspecific promoter complemented the seed P distribution phenotype of the pho1 phoh1 double mutant. CZSC-specific down-expression of PHO1 also recapitulated the seed P distribution phenotype of pho1. Together, these experiments show that PHO1 expression in the CZSC is important for the transfer of P from seed coat to the embryo in developing seeds. SummarySeed production requires the transfer of nutrients from the maternal seed coat to the filial endosperm and embryo. Since seed coat and filial tissues are symplasmically isolated, nutrients arriving in the seed coat via the phloem must be exported to the apoplast before reaching the embryo. Proteins implicated in the transfer of inorganic phosphate (Pi) from the seed coat to the embryo are unknown despite seed P content being an important agronomic trait. Here, we show that the Arabidopsis Pi exporter PHO1 and PHOH1 are expressed in the chalazal seed coat (CZSC) of developing seeds. PHO1 is additionally expressed in developing ovules. Phosphorus (P) content and Pi flux between seed coat and embryo was analyzed in seeds from grafts between WT roots and scions from either pho1, phoh1 or pho1 phoh1 double mutant. While P content and distribution between seed coat and embryo in fully mature dry seeds of these mutants are similar to WT, at the mature green stage of seed development the seed coat of the pho1 and pho1 phoh1 mutants, but not of the phoh1 mutant, retains approximately 2-fold more P than its WT control. Expression of PHO1 under a CZSCspecific promoter complemented the seed P distribution phenotype of the pho1 phoh1 double mutant. CZSC-specific down-expression of PHO1 also recapitulated the seed P distribution phenotype of pho1. Together, these experiments show that PHO1 expression in the CZSC is important for the transfer of P from seed coat to the embryo in developing seeds.

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“…For cereal grains, limited reports are available that describe the co-relation between Pi concentrations and its subsequent movement into the filial tissues1922. In wheat, two principal filial tissues, aleurone and endosperm are involved in a biochemical and transcriptional reprogramming during grain filling to facilitate nutrient transport3940.The total P concentration was found to be very high in aleurone as compared to endosperm of the 14 DAA seed, the stage when aleurone is discrete (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The regulation of PHT1 candidates involves the Pi starvation response regulators (PHR1) through their binding to cis -element P1BS (PHR1 binding sequence), SPX-proteins and PHO2 regulation151617. Studies in rice ( Oryzae sativa ) and Arabidopsis (Arabidopsis thaliana ) have suggested the role of PHT1 family genes in remobilization of P from senescing tissues to the actively developing tissues and their role in embryo development1819. In addition to that, Pi transport within the plant tissues is also contributed by PHT3 and PHT4 family members by regulating ‘intracellular Pi starvation’ signalling2021.…”

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confidence: 99%

Tissue specific transcript profiling of wheat phosphate transporter genes and its association with phosphate allocation in grains

Shukla

Kaur

Aggarwal

et al. 2016

Sci Rep

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Approaches enabling efficient phosphorus utilization in crops are of great importance. In cereal crop like wheat, utilization of inorganic phosphate (Pi) is high and mature grains are the major sink for Pi utilization and storage. Research that addresses the importance of the Pi homeostasis in developing grains is limited. In an attempt to understand the Pi homeostasis in developing wheat grains, we identified twelve new phosphate transporters (PHT), these are phyologentically well distributed along with the members reported from Arabidopsis and rice. Enhanced expression of PHT1-subfamily genes was observed in roots subjected to the Pi starvation suggesting their active role in Pi homeostasis. Differential expression patterns of all the PHT genes during grain filling stages suggested their importance in the filial tissues. Additionally, high accumulation of Pi and total P in aleurone correlates well with the expression of TaPHTs and other phosphate starvation related genes. Tissue specific transcript accumulation of TaPHT1.1, TaPHT1.2, TaPHT1.4 in aleurone; TaPHT3.1 in embryo and TaPHT4.2 in the endosperm was observed. Furthermore, their transcript abundance was affected in low phytate wheat grains. Altogether, this study helps in expanding the knowledge and prioritize the candidate wheat Pi-transporters to modulate the Pi homeostasis in cereal grains.

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Involvement ofOsPht1;4in phosphate acquisition and mobilization facilitates embryo development in rice

Cited by 125 publications

References 34 publications

A vacuolar phosphate transporter essential for phosphate homeostasis inArabidopsis

A vacuolar phosphate transporter essential for phosphate homeostasis inArabidopsis

PHO1 Exports Phosphate from the Chalazal Seed Coat to the Embryo in Developing Arabidopsis Seeds

Tissue specific transcript profiling of wheat phosphate transporter genes and its association with phosphate allocation in grains

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