Involvement of ICAM-1 in impaired spermatogenesis after busulfan treatment in mice

Abstract

Expression of adherence proteins, such as P-cadherin, has been identified in the normal testis and changed in impaired testis induced by alkylating agents. Intercellular adhesion molecule-1 (ICAM-1), a member of the immunoglobulin superfamily of cell adhesion molecules, is a constituent component of the blood-testis barrier and a multifunctional molecule in homeostasis of spermatogenesis. However, the distribution of ICAM-1 in the testis of mice and expression changes after busulfan treatment remain unclear. In this study, ICAM-1 immunoreaction was detected in Sertoli and germinal cells, particularly in spermatogonia, and elongating and elongated spermatids of normal testes. Accompanied with degeneration of spermatogenesis (decrease in testicular and epididymal weights, as well as loss of germ cells in histological morphology), ICAM-1 expression declined significantly in the seminiferous tubules during a 4-week experimental period, particularly in the first 2 weeks (40 mg kg(-1) busulfan, single injection). Compared with the control group, busulphan-treated testes showed a significant increase in lipid peroxidation during weeks 1 and 2. Thus, ICAM-1 may play an important role in the homeostasis of spermatogenesis, and busulfan treatment can lead to adhesion disintegration.

Mesenchymal stem cell‐secreted factors delayed spermatogenesis injuries induced by busulfan involving intercellular adhesion molecule regulation

“…Our results found that MSC-secreted factors promoted ICAM-1，N-cadherin and P-cadherin expressions in vivo, suggesting maintaining BTB integrity and cell adhesion might be the significant way in the crosstalk of MSC and spermatogenic microenvironment. Immunolocalisation studies showed ICAM-1 to be present at Sertoli cells and germinal cells (Cai, Liu, Fang, Shen, & Xiong, 2016), particularly at the site of the BTB, coinciding with two critical cellular events that occur: the movement of preleptotene/leptotene spermatocytes across the BTB and the release of elongated spermatids into the tubule lumen (Xiao et al, 2012). Immunolocalisation studies showed ICAM-1 to be present at Sertoli cells and germinal cells (Cai, Liu, Fang, Shen, & Xiong, 2016), particularly at the site of the BTB, coinciding with two critical cellular events that occur: the movement of preleptotene/leptotene spermatocytes across the BTB and the release of elongated spermatids into the tubule lumen (Xiao et al, 2012).…”

“…P-cadherin primarily locates in peritubular myoid cells of the interstitial tissue of mouse testis (Lin & DePhilip, 1996). Immunolocalisation studies showed ICAM-1 to be present at Sertoli cells and germinal cells (Cai, Liu, Fang, Shen, & Xiong, 2016), particularly at the site of the BTB, coinciding with two critical cellular events that occur: the movement of preleptotene/leptotene spermatocytes across the BTB and the release of elongated spermatids into the tubule lumen (Xiao et al, 2012). Ncadherin localises in spermatogonia, primary spermatocytes and Sertoli cells (Tsuchiya, Sato, Kameya, Okayasu, & Mukai, 2006).…”

Busulfan administration produces toxic effects on epididymal morphology and inhibits the expression of ZO-1 and vimentin in the mouse epididymis

“…There is growing evidence that busulfan treatment causes deleterious effects on cell junctions and the cytoskeleton. Intercellular adhesion molecule-1 (ICAM-1) is involved in impaired spermatogenesis after busulfan treatment in mice [ 15 ]. The expression of P-cadherin decreases when spermatocytes disappear after busulfan treatment [ 16 ].…”

>b<Conservação de germoplasma de cracídeos ameaçados por meio de transplantes interespecíficos de células germinativas>/b<

“…Isto resulta no mau pareamento das bases resultando no bloqueio da replicação, transcrição do DNA e na inibição da proliferação e diferenciação celular (Iwamoto et al, 2004). Além disso, o bussulfano tem a capacidade de destruir os filamentos de vimentina que formam as junções justapostas entre as células de Sertoli afetando a estabilidade do nicho testicular das SSCs (Amann, 2008;Cai et al, 2016 (Blesbois, 2007;Kang et al, 2008;Song e Silversides, 2008). Consequentemente, a criopreservação de sêmen é atualmente o procedimento mais adequado para o banco de germoplasma em espécies aviárias (Blesbois, 2007;Woelders et al, 2006).…”