Involvement of intrinsic mitochondrial pathway in neosergeolide-induced apoptosis of human HL-60 leukemia cells: The role of mitochondrial permeability transition pore and DNA damage

Cavalcanti, Bruno C.; Costa, Patrícia Marçal da; Carvalho, Adriana Andrade; Rodrigues, Felipe Augusto Rocha; Amorim, R.C.N.; Silva, Ellen C. C.; Pohlit, Adrian Martin; Costa-Lotufo, Letı́cia V.; Moraes, Manoel O. de; Pessoa, Cláudia

doi:10.3109/13880209.2012.654921

Cited by 21 publications

(11 citation statements)

References 81 publications

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“…The Annexin V cytometry assay was used to detect cell population in viable, early and late apoptosis stage as described by Cavalcanti and coworkers [65]. After short exposure time (6 h) with compounds 21 and 22 at 5 μM, PC3 cells were stained with fluorescein isothiocyanate (FITC) conjugated Annexin V (Guava Nexin kit, Guava Technologies, Inc., Hayward, CA, USA) and PI (necrotic-cell indicator), and then they were subjected to flow cytometry (Guava EasyCyte Mini).…”

Section: Methodsmentioning

confidence: 99%

Synthesis and antitumor activity of selenium-containing quinone-based triazoles possessing two redox centres, and their mechanistic insights

Cruz

Silvers

Jardim

et al. 2016

European Journal of Medicinal Chemistry

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Selenium-containing quinone-based 1,2,3-triazoles were synthesized using click chemistry, the copper catalyzed azide-alkyne 1,3-dipolar cycloaddition, and evaluated against six types of cancer cell lines: HL-60 (human promyelocytic leukemia cells), HCT-116 (human colon carcinoma cells), PC3 (human prostate cells), SF295 (human glioblastoma cells), MDA-MB-435 (melanoma cells) and OVCAR-8 (human ovarian carcinoma cells). Some compounds showed IC50 values < 0.3 μM. The cytotoxic potential of the quinones evaluated was also assayed using non-tumor cells, exemplified by peripheral blood mononuclear (PBMC), V79 and L929 cells. Mechanistic role for NAD(P)H:Quinone Oxidoreductase 1 (NQO1) was also elucidated. These compounds could provide promising new lead derivatives for more potent anticancer drug development and delivery, and represent one of the most active classes of lapachones reported.

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Section: Methodsmentioning

confidence: 99%

Synthesis and antitumor activity of selenium-containing quinone-based triazoles possessing two redox centres, and their mechanistic insights

Cruz

Silvers

Jardim

et al. 2016

European Journal of Medicinal Chemistry

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“…The loss of MMP causes an increase in the permeability of the mitochondrial membrane, followed by the release of proapoptotic molecules such as cytochrome c (Cavalcanti et al, 2012;Huang et al, 2013). Cytochrome c releasing from mitochondrial interacts with ATP, Apaf-1, and caspase-9, and subsequently activates caspase-3, which consequently elicits caspase-dependent apoptotic cell death.…”

Section: Discussionmentioning

confidence: 99%

Leonurine hydrochloride induces apoptosis of H292 lung cancer cell by a mitochondria-dependent pathway

Mao

Zhang

Cai

et al. 2015

Pharmaceutical Biology

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(2015) Leonurine hydrochloride induces apoptosis of H292 lung cancer cell by a mitochondria-dependent pathway, Pharmaceutical Biology, 53:11, 1684-1690, DOI: 10.3109/13880209.2014 Objective: This study investigated its effects on human non-small cell lung cancer (NSCLC) H292 cells and illustrated the possible mechanism involved. Materials and methods: After treatment with different concentrations of LH (0, 10, 25, and 50 mmol/L) for 6, 12, 24, 48, and 72 h, the cell viability was assessed by the MTT assay. After exposed to different doses of LH for 24 h, cell-cycle distribution, cell apoptosis, mitochondrial membrane potential (MMP), and reactive oxygen species (ROS) were monitored by flow cytometry. RT-PCR and western blot were used to detect the expression of apoptosis-related genes.Results: LH significantly inhibited the proliferation of H292 cells in a time-and dose-dependent manner, and induced G0/G1 cell-cycle arrest. Coincidentally, LH treatment at a dose of 10, 25, and 50 mmol/L for 24 h increased apoptotic ratio from 4.9 ± 0.43% to 11.5 ± 1.12%, 19.3 ± 1.16%, and 61.3 ± 6.69%, respectively. The inhibition effect of LH on H292 cells was associated with the loss of MMP and the generation of ROS. The phosphorylation level of p38 was increased and Akt phosphorylation was reduced by LH treatment. Furthermore, LH treatment increased the expression levels of caspase-3, caspase-9 and Bax/Bcl-2. Conclusions: LH inhibits the proliferation and induces the apoptosis of H292 cells in a mitochondria-dependent pathway, and the specific mechanism need to be further explored.

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“…The cells were then analyzed by flow cytometry (Guava EasyCyte minisystem). For each experiment (n ϭ 3), 10,000 events were evaluated, and cell debris was omitted from the analysis (38).…”

Section: Cell Treatmentsmentioning

confidence: 99%

Synergistic Effect of the Flavonoid Catechin, Quercetin, or Epigallocatechin Gallate with Fluconazole Induces Apoptosis in Candida tropicalis Resistant to Fluconazole

Silva

Neto

Campos

et al. 2014

Antimicrob Agents Chemother

110

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Involvement of intrinsic mitochondrial pathway in neosergeolide-induced apoptosis of human HL-60 leukemia cells: The role of mitochondrial permeability transition pore and DNA damage

Cited by 21 publications

References 81 publications

Synthesis and antitumor activity of selenium-containing quinone-based triazoles possessing two redox centres, and their mechanistic insights

Synthesis and antitumor activity of selenium-containing quinone-based triazoles possessing two redox centres, and their mechanistic insights

Leonurine hydrochloride induces apoptosis of H292 lung cancer cell by a mitochondria-dependent pathway

Synergistic Effect of the Flavonoid Catechin, Quercetin, or Epigallocatechin Gallate with Fluconazole Induces Apoptosis in Candida tropicalis Resistant to Fluconazole

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