Involvement of lipopeptide antibiotics and chitinase genes and induction of host defense in suppression of Fusarium wilt by endophytic Bacillus spp. in tomato

Abdallah, Rania Aydi Ben; Stedel, Catalina; Garagounis, Constantine; Nefzi, Adel; Jabnoun‐Khiareddine, Hayfa; Papadopoulou, Kalliope Κ.; Daami‐Remadi, Mejda

doi:10.1016/j.cropro.2017.05.008

Cited by 73 publications

(30 citation statements)

References 39 publications

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“…Our results indicate that the enhanced activities of defense-related enzymes may play a significant role in the resistance of peaches to the pathogens infection and the induced activity of defense-related enzymes to be part of the mechanism of B. subtilis JK-14 in controlling postharvest diseases of peach fruits. Some previous studies revealed that one of the important mechanisms for the genus Bacillus in controlling plant diseases is by increasing and activating the plant systemic resistance [45,46,47]. In addition, the enhanced activities of antioxidant enzymes (SOD, POD, CAT and ascorbate peroxidase, APX) and their coordinated action have been reported to be a part of the mechanism implicated in the alleviation of lipid peroxidation and delay of senescence in peach fruits [48].…”

Section: Discussionmentioning

confidence: 99%

Identification of the Fungal Pathogens of Postharvest Disease on Peach Fruits and the Control Mechanisms of Bacillus subtilis JK-14

Zhang

Zheng²,

Xu³

et al. 2019

Toxins

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Postharvest fungal disease is one of the significant factors that limits the storage period and marketing life of peaches, and even result in serious economic losses worldwide. Biological control using microbial antagonists has been explored as an alternative approach for the management of postharvest disease of fruits. However, there is little information available regarding to the identification the fungal pathogen species that cause the postharvest peach diseases and the potential and mechanisms of using the Bacillus subtilis JK-14 to control postharvest peach diseases. In the present study, a total of six fungal isolates were isolated from peach fruits, and the isolates of Alternaria tenuis and Botrytis cinerea exhibited the highest pathogenicity and virulence on the host of mature peaches. In the culture plates, the strain of B. subtilis JK-14 showed the significant antagonistic activity against the growth of A. tenuis and B. cinerea with the inhibitory rates of 81.32% and 83.45% at 5 days after incubation, respectively. Peach fruits treated with different formulations of B. subtilis JK-14 significantly reduced the mean disease incidences and lesion diameters of A. tenuis and B. cinerea. The greatest mean percent reduction of the disease incidences (81.99% and 71.34%) and lesion diameters (82.80% and 73.57%) of A. tenuis and B. cinerea were obtained at the concentration of 1 × 107 CFU mL−1 (colony forming unit, CFU). Treatment with the strain of B. subtilis JK-14 effectively enhanced the activity of the antioxidant enzymes-superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in A. tenuis and B. cinerea inoculated peach fruits. As such, the average activities of SOD, POD and CAT were increased by 36.56%, 17.63% and 20.35%, respectively, compared to the sterile water treatment. Our results indicate that the isolates of A. tenuis and B. cinerea are the main pathogens that cause the postharvest peach diseases, and the strain of B. subtilis JK-14 can be considered as an environmentally-safe biological control agent for the management of postharvest fruits diseases. We propose the possible mechanisms of the strain of B. subtilis JK-14 in controlling of postharvest peach diseases.

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Section: Discussionmentioning

confidence: 99%

Identification of the Fungal Pathogens of Postharvest Disease on Peach Fruits and the Control Mechanisms of Bacillus subtilis JK-14

Zhang

Zheng²,

Xu³

et al. 2019

Toxins

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“…Moreover, this chitinase isoform seems to be involved in plant defense responses, since it is highly expressed in plants infected with the pathogenic fungus Fusarium oxysporum f. sp. Lycopersici [52].…”

Section: Discussionmentioning

confidence: 99%

Foliar Application of Chitosan Increases Tomato Growth and Influences Mycorrhization and Expression of Endochitinase-Encoding Genes

Amerany

Meddich

Wahbi

et al. 2020

IJMS

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Nowadays, applying bio-organic fertilizer (e.g., chitosan, Ch) or integrating beneficial microorganisms (e.g., arbuscular mycorrhizal fungi, AMF) are among the successful strategies to promote plant growth. Here, the effect of two application modes of Ch (foliar spray or root treatment) and Ch-derived nanoparticles (NPs) on tomato plants colonized with the AMF Rhizophagus irregularis were analyzed, thereby focusing on plant biomass, flowering and mycorrhization. An increase of shoot biomass and flower number was observed in arbuscular mycorrhizal (AM) plants sprayed with Ch. The interaction with AMF, however, was reduced as shown by decreased mycorrhization rates and AM-specific gene expression. To get insights into Ch effect on mycorrhization, levels of sugars, jasmonates, abscisic acid, and the expression of two chitinase-encoding genes were determined in mycorrhizal roots. Ch had no effect on sugar and phytohormone levels, but the reduced mycorrhization was correlated with down- and upregulated expression of Chi3 and Chi9, respectively. In contrast, application of NPs to leaves and Ch applied to the soil did not show any effect, neither on mycorrhization rate nor on growth of mycorrhizal plants. Concluding, Ch application to leaves enhanced plant growth and flowering and reduced interaction with AMF, whereas root treatment did not affect these parameters.

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“…B. subtilis SV41 and B. amyloliquefaciens subsp. plantarum SV65 were previously tested with other Bacillus species in a pot experiment where authors indicated that Fusarium wilt severity suppression was significantly correlated to the decreaseof pathogen colonization of vascular tissues leading consequently to plant growth promotion (Aydi Ben Abdallah et al, 2017). Indeed, B. subtilis SV41 was found the highest salicylic acid producing agent that acts as an elicitor for the induction of systemic resistance against pathogen attack.…”

Section: Discussionmentioning

confidence: 99%

“…Also, the plant defense genes such as PR1, acidic PR3 and lipoxygenase (LOXD) were expressed in tomato plants inoculated or not with FOL and treated with B. amyloliquefaciens subsp. plantarum SV65 (Aydi Ben Abdallah et al, 2017). Furthermore, the plant growthpromoting traits such as the production of indole-3-acetic acid, siderophores and organic acids in root exudates and the phosphate solubilization ability were recorded on both selected strains (unpublished data).…”

Section: Bacterial Materialsmentioning

confidence: 99%

Field suppression of Fusarium wilt and microbial population Shifts in tomato rhizosphere following soil treatment with two selected endophytic bacteria

Abdallah¹,

Jabnoun‐Khiareddine²,

Nefzi³

et al. 2019

Eurasian Journal of Soil Science (Ejss)

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Two endophytic bacteria, Bacillus subtilis SV41 (KR818071) and B. amyloliquefaciens subsp. plantarum SV65 (KR818073), were assessed under field conditions for their capacity to control tomato Fusarium wilt in tomato and their effects on soil microbial activity. Six months after planting, Fusarium wilt severity, estimated through the vascular browning extent in tomato stems, was significantly reduced by 82.3 and 88.2% compared to control following bacterial treatments. The frequency of F.oxysporum reisolation from roots, collars and stems was also significantly lowered in treated plants compared to controls. These effects were associated with a significant improvement, by 10.6 to 16.3%over control, in plant height and root fresh weight and an increase in fruit production by 8.4-12.5%. As for microbial activity, F. oxysporum population in the rhizosphere of tomato plants treated with B. subtilis SV41 and B. amyloliquefaciens subsp. plantarum SV65 was reduced by 87.5-91.7%compared to the initial soil (sampled before planting) and by 88.4-92.3% relative to the rhizospheric soil of untreated plants (control soil). A significant enhancement in the total culturable bacterial community was also noted in the rhizosphere of tomato plants treated with both strains compared to initial and control soils where a significant enrichment in Pseudomonas and actinobacteria community was recorded.

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Involvement of lipopeptide antibiotics and chitinase genes and induction of host defense in suppression of Fusarium wilt by endophytic Bacillus spp. in tomato

Cited by 73 publications

References 39 publications

Identification of the Fungal Pathogens of Postharvest Disease on Peach Fruits and the Control Mechanisms of Bacillus subtilis JK-14

Identification of the Fungal Pathogens of Postharvest Disease on Peach Fruits and the Control Mechanisms of Bacillus subtilis JK-14

Foliar Application of Chitosan Increases Tomato Growth and Influences Mycorrhization and Expression of Endochitinase-Encoding Genes

Field suppression of Fusarium wilt and microbial population Shifts in tomato rhizosphere following soil treatment with two selected endophytic bacteria

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