Involvement of MAK-1 and MAK-2 MAP kinases in cell wall integrity in <i>Neurospora crassa</i>

Kamei, Masayuki; Yamashita, Kazuhiro; Takahashi, Masakazu; Fukumori, Fumiyasu; Ichiishi, Akihiko; Fujimura, Masatake

doi:10.1080/09168451.2016.1189321

Cited by 11 publications

(4 citation statements)

References 68 publications

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“…The most interesting thing is that both phosphorylated and total MAK-1 proteins were undetected in all deletion mutants of kinases belonging to the PR-MAP kinase pathway (the nrc-1, mek-2, and mak-2 mutants) during protoperithecium development. This is similar to the result of Kamei et al (2016), in which the phosphorylation of MAK-1 was undetected in the mak-2 mutant from conidial germination to hyphal development. Recent studies showed that proper fruiting body development in N. crassa was dependent on the MAK-2-phosphorylated N-terminus of MOB-3, a member protein of the STRIPAK complex, which impacts the nuclear accumulation of MAK-1 (Dettmann et al, 2013).…”

Section: Discussionsupporting

confidence: 88%

Coordinated Regulation of Protoperithecium Development by MAP Kinases MAK-1 and MAK-2 in Neurospora crassa

et al. 2021

Front. Microbiol.

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Mitogen-activated protein (MAP) kinase pathways function as signaling hubs that are integral for many essential cellular processes, including sexual development. The molecular mechanisms and cross-talk between PR and CWI MAP kinase pathways have been extensively studied during asexual development. However, if these can be extended to sexual development remains elusive. By analyzing genome-wide transcriptional responses to deletion of each of two MAP kinase coding genes mak-2 (PR-MAP kinase pathway) and mak-1 (CWI-MAP kinase pathway) in Neurospora crassa during protoperithecium formation, 430 genes co-regulated by the MAK-1 and MAK-2 proteins were found, functionally enriched at integral components of membrane and oxidoreductase. These genes include 13 functionally known genes participating in sexual development (app, poi-2, stk-17, fsd-1, vsd-8, and NCU03863) and melanin synthesis (per-1, pkh-1, pkh-2, mld-1, scy-1, trn-2, and trn-1), as well as a set of functionally unknown genes. Phenotypic analysis of deletion mutants for the functionally unknown genes revealed that 12 genes were essential for female fertility. Among them, single-gene deletion mutants for NCU07743 (named as pfd-1), NCU02250 (oli), and NCU05948 (named as pfd-2) displayed similar protoperithecium development defects as the Δmak-1 and Δmak-2 mutants, failing to form protoperithecium. Western blotting analysis showed that both phosphorylated and total MAK-1 proteins were virtually abolished in the Δnrc-1, Δmek-2, and Δmak-2 mutants, suggesting that the posttranscriptional regulation of MAK-1 is dependent on the PR-MAP kinase pathway during the protoperithecium development. Taken together, this study revealed the regulatory roles and cross-talk between PR and CWI-MAP kinase pathways during protoperithecium development.

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Section: Discussionsupporting

confidence: 88%

Coordinated Regulation of Protoperithecium Development by MAP Kinases MAK-1 and MAK-2 in Neurospora crassa

et al. 2021

Front. Microbiol.

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“…Furthermore, mitogen‐activated protein kinases (MAP kinases) are known to be involved in multifunctional and diverse signalling pathways, including cell wall integrity [26], cell‐to‐cell communication [13], circadian regulation of cellular processes [11, 12], phosphate signalling [27] and sensing of osmolarity and carbon [28] in N. crassa . We confirmed that three MAP kinases had been identified with existing acetylation sites, including MAK‐1, MAK‐2, and HOG‐1, indicating that these three MAP kinases may have been acetylated to participate in regulating multifunctional and various signalling pathways.…”

Section: Figurementioning

confidence: 99%

“…To better ascertain the functions of acetylation proteins, we further classified the identified proteins involvement in diverse signalling pathways (Table S2) Furthermore, mitogen-activated protein kinases (MAP kinases) are known to be involved in multifunctional and diverse signalling pathways, including cell wall integrity [26], cell-to-cell communication [13], circadian regulation of cellular processes [11,12], phosphate signalling [27] and sensing of osmolarity and carbon [28] in N. crassa. We con- Moreover, we identified the casein kinases, including the casein kinase I isoform delta (CK-1a), casein kinase 1b (CK-1b) and casein kinase II subunit alpha (CKα) has having acetylation sites, and interestingly, CK-1a had nine acetylation sites distributed in the N terminus.…”

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confidence: 99%

Proteome‐wide analyses reveal diverse functions of acetylation proteins in Neurospora crassa

Wang

Guan

et al. 2021

Proteomics

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Quantitative acetyl-proteomics, a newly identified post-translational modification, is known to regulate transcriptional activity in different organisms. Neurospora crassa is a model ascomycete fungus maintained for biochemistry and molecular biology research; however, extensive studies of the functions of its acylation proteins have yet to be performed. In this study, using LC-MS/MS qualitative proteomics strategies, we identified 1909 modification sites on 940 proteins in N. crassa and analysed the functions of these proteins using GO enrichment, KEGG pathway, and subcellular location experiments. We classified the acetylation protein involvement in diverse pathways, and protein-protein interaction (PPI) network analysis further demonstrated that these proteins participate in diverse biological processes. In summary, our study comprehensively profiles the crosstalk of modified sites, and PPI among these proteins may form a complex network with both similar and distinct regulatory mechanisms, providing improved understanding of their biological functions in N. crassa. K E Y W O R D S acetylated proteins, Neurospora crassa, protein-protein interaction, quantitative acetylproteomics Neurospora crassa is an ascomycete fungus that has been used extensively in modern genetics, biochemistry and molecular biology research, maintaining a central role as a model organism for more than 80 years [1, 2]. Neurospora was first discovered as a contaminant in bakeries, and approximately 80 years later it was developed as an experimental organism in the 1920s [3-6]. In the latter 50 years, Neurospora has been used as a model fungus and has contributed to our fundamental understanding of genomic defines systems, such as DNA repair, DNA methylation, post-transcriptional gene silencing, mitochondrial protein import and circadian rhythms [7]. Moreover, Neurospora has also been used as a model system to study cellular differentiation and development, as well as other aspects of eukaryotic biology [2]. Subsequently, a high-quality draft sequence of the N. crassa genome has aided in efforts to investigative the molecular biology and genetics of filamentous fungi [8]. Studies of the genes regulating various aspects of Neurospora biology include the cAMP-dependent and heterotrimeric G-protein pathways [9, 10], mitogen-activated protein kinase pathway [11-13], light photobiology and circadian rhythmicity [14-17], secondary metabolism [18-20], cell wall structure and synthesis [21] and so on. With respect to N. crassa DNA, approximately 1.5% of the cytosines are methylated, facilitating genetic studies [22]. However, to

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“…Ssd1 functions in parallel to Mpt5 (another RBP) to maintain CWI (Kaeberlein and Guarente, 2002 ). In N. crassa , the conserved CWI pathway is activated under cell-wall stress conditions and modulated by different proteins, including a Mitogen-Activated Protein-Kinase (MAPK) cascade that is comprised of MIK-1 (NCU02234), MEK-1 (NCU06419), and MAK-1 (NCU09842) (Park et al, 2008 ; Bennett et al, 2013 ; Kamei et al, 2016 ). This pathway can alter the transcription of multiple genes via the transcription-factor ADV-1 (NCU07392), encoded by a gene that is conserved among the Pezizomycotina and is essential for germling communication and fusion (Leeder et al, 2013 ; Dekhang et al, 2017 ; Fischer et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

The GUL-1 Protein Binds Multiple RNAs Involved in Cell Wall Remodeling and Affects the MAK-1 Pathway in Neurospora crassa

Herold

Zolti

Garduño-Rosales

et al. 2021

Front. Fungal Biol.

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The Neurospora crassa GUL-1 is part of the COT-1 pathway, which plays key roles in regulating polar hyphal growth and cell wall remodeling. We show that GUL-1 is a bona fide RNA-binding protein (RBP) that can associate with 828 “core” mRNA species. When cell wall integrity (CWI) is challenged, expression of over 25% of genomic RNA species are modulated (2,628 mRNAs, including the GUL-1 mRNA). GUL-1 binds mRNAs of genes related to translation, cell wall remodeling, circadian clock, endoplasmic reticulum (ER), as well as CWI and MAPK pathway components. GUL-1 interacts with over 100 different proteins, including stress-granule and P-body proteins, ER components and components of the MAPK, COT-1, and STRIPAK complexes. Several additional RBPs were also shown to physically interact with GUL-1. Under stress conditions, GUL-1 can localize to the ER and affect the CWI pathway—evident via altered phosphorylation levels of MAK-1, interaction with mak-1 transcript, and involvement in the expression level of the transcription factor adv-1. We conclude that GUL-1 functions in multiple cellular processes, including the regulation of cell wall remodeling, via a mechanism associated with the MAK-1 pathway and stress-response.

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Involvement of MAK-1 and MAK-2 MAP kinases in cell wall integrity in Neurospora crassa

Cited by 11 publications

References 68 publications

Coordinated Regulation of Protoperithecium Development by MAP Kinases MAK-1 and MAK-2 in Neurospora crassa

Coordinated Regulation of Protoperithecium Development by MAP Kinases MAK-1 and MAK-2 in Neurospora crassa

Proteome‐wide analyses reveal diverse functions of acetylation proteins in Neurospora crassa

The GUL-1 Protein Binds Multiple RNAs Involved in Cell Wall Remodeling and Affects the MAK-1 Pathway in Neurospora crassa

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