Involvement of MicroRNA-296 in the Inhibitory Effect of Epigallocatechin Gallate against the Migratory Properties of Anoikis-Resistant Nasopharyngeal Carcinoma Cells

Abstract

Short noncoding endogenous RNAs, including microRNAs (miRNAs), are associated with the development and metastasis of multiple cancers. Epigallocatechin gallate (EGCG), the most active and abundant polyphenol in green tea, plays a crucial role in the modulation of miRNA expression, which is related to changes in cancer progression. In the present study, we explore whether EGCG exerts its suppressive effects on nasopharyngeal carcinoma (NPC) cells through miRNA regulation. The anoikis-resistant sphere-forming NPC cells grown under anchorage-independent conditions exhibit enhanced migratory properties, which were inhibited by EGCG treatment. The miR-296 level was lower in the anoikis-resistant cells than in the monolayer parental cells; however, miR-296 was significantly upregulated after EGCG treatment. We demonstrate that miR-296 is involved in the inhibitory effects of EGCG on the anoikis-resistant NPC cells through the downregulation of signal transducer and activator of transcription 3 (STAT3) activation. Our study is the first to demonstrate that EGCG inhibited the migratory properties of anoikis-resistant cells by modulating the expression of miRNA in NPC cells. Our results indicate the novel effects of EGCG on miRNA regulation to inhibit an invasive phenotype of NPC as well as the regulatory role of miR-296.

The pleiotropic role of transcription factor STAT3 in oncogenesis and its targeting through natural products for cancer prevention and therapy

“…Epigallocatechin‐3‐gallate (EGCG), a major polyphenolic constituent of green tea, possesses remarkable chemo‐preventive and therapeutic potential against various types of cancer 298 . EGCG treatment significantly upregulated miR‐296 and implicated in the inhibitory effects on the migratory properties of anoikis‐resistant nasopharyngeal carcinoma cells through the downregulation of STAT3 activation 299 . EGCG in imatinib‐sensitive and imatinib‐resistant Bcr/Abl+ cells, especially T315I‐mutated CML inhibited cell growth and induced apoptosis by downregulating JAK2/STAT3/AKT pathways 300 .…”

Two Worlds Colliding: The Interplay Between Natural Compounds and Non-Coding Transcripts in Cancer Therapy

“…In nasopharyngeal carcinoma cells, EGCG modulated miR-296 and decreased cancer cell migration. This effect was associated with down-regulation of signal transducer and activator of transcription 3 (STAT3) activation mediated by miR-296 ( Lin et al, 2020 ).…”

The Impact of Dietary Compounds in Functional Foods on MicroRNAs Expression

“…EGCG is a powerful antioxidant, anticancer and antiangiogenic properties, which has a potential role to influence human diseases [54][55][56]. EGCG suppressed nasopharyngeal carcinoma cell migration and invasion through a novel signaling axis of miR-296/STAT3 regulation [57]. Gold nanoparticles (AuNPs) have been used for drug delivery as their stability and increase drug bioavailability as well as accumulation of drug in cancer cells.…”