Involvement of microRNA164 in responses to heat stress in Arabidopsis

Tsai, Wei-An; Sung, Po-Han; Kuo, Yun‐Wei; Chen, Ming-Cheng; Jeng, Shih‐Tong; Lin, Jer-Sheng

doi:10.1016/j.plantsci.2023.111598

Cited by 12 publications

(9 citation statements)

References 72 publications

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“…However, randomly located CUC2 expression domains were occasionally observed in a triple mir164abc mutant, suggesting that collectively the 3 MIR164 genes may also contribute to robustness of their target expression patterns (Sieber et al, 2007). Nevertheless, the role of individual MIR164 genes in robustness was not established and, more precisely, the role of MIR164B during plant development was not known, though MIR164B was shown to be involved in the physiological response of plants to environmental stresses, but possibly through targets different from CUC1/CUC2 (Du et al, 2022; Tsai et al, 2023). Here we show that MIR164B acts as a safe guard to counteract transcriptional deregulation of its target CUC2.…”

Section: Discussionmentioning

confidence: 99%

Compensation of compromised PRC2 regulation by a miRNA ensures robustness of Arabidopsis leaf development

Maugarny,

Vialette,

Adroher

et al. 2023

Preprint

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Robustness is pervasive throughout biological systems, enabling them to maintain persistent outputs despite perturbations in their components. Here, we reveal a novel mechanism contributing to leaf morphology robustness in the face of genetic perturbations. In Arabidopsis, leaf shape is established during early development through the quantitative action of the CUP-SHAPED COTYLEDON2 (CUC2) gene that is negatively regulated by the co-expressed MICRORNA164A (MIR164A) gene. Compromised epigenetic regulation due to defective Polycomb Repressive Complex 2 (PRC2) function results in the transcriptional derepression of CUC2 but has no impact on CUC2 protein dynamics or early morphogenesis. We solve this apparent paradox by showing that compromised PRC2 function simultaneously activates a compensatory mechanism involving another member of the MIR164 gene family, the MIR164B gene. This mechanism dampens CUC2 protein levels, thereby compensating for compromised PRC2 function and canalizing early leaf morphogenesis. Furthermore, we show that this compensation mechanism is active under different environmental conditions. Our findings shed light on how the interplay between different types of transcriptional regulation can contribute to developmental robustness.

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Section: Discussionmentioning

confidence: 99%

Compensation of compromised PRC2 regulation by a miRNA ensures robustness of Arabidopsis leaf development

Maugarny,

Vialette,

Adroher

et al. 2023

Preprint

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“…miRNAs are small regulators playing important roles in multiple physiological responses (Lin et al, 2018; Kuo et al, 2019; Tsai et al, 2023). In particular, miR163 has been shown to be involved in seed germination, primary root formation, and biotic stress resistance (Ng et al, 2011; Chung et al, 2016; Chow & Ng, 2017; Li et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, the GO analysis indicated that miR163 might play a role in regulating plant responses to abiotic stresses such as cold, osmotic stress, heat, and oxidative stress (Figure 2D). Like miR156, miR160 and miR164 are known to regulate plant development and stress responses (Lin et al, 2018; Li et al, 2019; Tsai et al, 2023). Additionally, PlantPAN 3.0 analysis (Chow et al 2019) revealed that several ABA‐responsive transcription factors, including AFB1, AFB3, AFB4, HSFA6A, and RD26, might bind to the miR163 promoter region.…”

Section: Discussionmentioning

confidence: 99%

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Regulatory roles of microRNA163 in responses to stresses in Arabidopsis

Kok,

Kuo,

Soh

et al. 2023

Physiologia Plantarum

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MicroRNAs (miRNAs) are small regulatory RNAs that participate in various biological processes by silencing target genes. In Arabidopsis, microRNA163 (miR163) was found to be involved in seed germination, root development, and biotic resistance. However, the regulatory roles of miR163 remain unclear. In the current study, the mir163 mutant was investigated to comprehensively understand and characterize its functions in Arabidopsis. RNA‐sequencing and Gene Ontology enrichment analyses revealed that miR163 might be involved in “response to stimulus” and “metabolic process”. Interestingly, “response to stress”, including heat, cold, and oxidative stress, was enriched under the subcategory of “response to stimulus”. We observed that miR163 and PXMT were repressed and induced under heat stress, respectively. Furthermore, the study detected significant differences in seed germination rate, hypocotyl length, and survival rate, indicating a variation in the thermotolerance between WT and mir163 mutant. The results revealed that the mir163 mutant had a lesser degree of germination inhibition by heat treatment than WT. In addition, the mir163 mutant showed a better survival rate and longer hypocotyl length under heat treatment than the WT. The metabolomes of WT and mir163 mutant were further analyzed. The contents of benzene derivatives and flavonoids were affected by miR163, which could enhance plants' defense abilities. In conclusion, miR163/targets regulated the expression of stress‐responsive genes and the accumulation of defense‐related metabolites to alter stress tolerance.

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“…The infiltrated discs were rinsed with sterile water and placed on 0.4% agar medium at 24 °C for 72 h. For heat shock treatment, the discs were exposed to 40 °C for 16 h. Heat stress can impact chlorophyll content, resulting in a reduction thereof. The extent of damage to chlorophyll is indicative of heat tolerance [ 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 ]. The activities of SOD and CAT enzymes were assessed following a 3 h heat treatment, while the levels of Malondialdehyde (MDA) and H 2 O 2 were determined after a 16 h period.…”

Section: Methodsmentioning

confidence: 99%

BcWRKY22 Activates BcCAT2 to Enhance Catalase (CAT) Activity and Reduce Hydrogen Peroxide (H2O2) Accumulation, Promoting Thermotolerance in Non-Heading Chinese Cabbage (Brassica campestris ssp. chinensis)

Wang,

Gao,

Chen

et al. 2023

Antioxidants

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WRKY transcription factors (TFs) participate in plant defense mechanisms against biological and abiotic stresses. However, their regulatory role in heat resistance is still unclear in non-heading Chinese cabbage. Here, we identified the WRKY-IIe gene BcWRKY22(BraC09g001080.1), which is activated under high temperatures and plays an active role in regulating thermal stability, through transcriptome analysis. We further discovered that the BcWRKY22 protein is located in the nucleus and demonstrates transactivation activity in both the yeast and plant. Additionally, our studies showed that the transient overexpression of BcWRKY22 in non-heading Chinese cabbage activates the expression of catalase 2 (BcCAT2), enhances CAT enzyme activity, and reduces Hydrogen Peroxide (H2O2) accumulation under heat stress conditions. In addition, compared to its wild-type (WT) counterparts, Arabidopsis thaliana heterologously overexpresses BcWRKY22, improving thermotolerance. When the BcWRKY22 transgenic root was obtained, under heat stress, the accumulation of H2O2 was reduced, while the expression of catalase 2 (BcCAT2) was upregulated, thereby enhancing CAT enzyme activity. Further analysis revealed that BcWRKY22 directly activates the expression of BcCAT2 (BraC08g016240.1) by binding to the W-box element distributed within the promoter region of BcCAT2. Collectively, our findings suggest that BcWRKY22 may serve as a novel regulator of the heat stress response in non-heading Chinese cabbage, actively contributing to the establishment of thermal tolerance by upregulating catalase (CAT) activity and downregulating H2O2 accumulation via BcCAT2 expression.

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Involvement of microRNA164 in responses to heat stress in Arabidopsis

Cited by 12 publications

References 72 publications

Compensation of compromised PRC2 regulation by a miRNA ensures robustness of Arabidopsis leaf development

Compensation of compromised PRC2 regulation by a miRNA ensures robustness of Arabidopsis leaf development

Regulatory roles of microRNA163 in responses to stresses in Arabidopsis

BcWRKY22 Activates BcCAT2 to Enhance Catalase (CAT) Activity and Reduce Hydrogen Peroxide (H2O2) Accumulation, Promoting Thermotolerance in Non-Heading Chinese Cabbage (Brassica campestris ssp. chinensis)

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