Involvement of mitochondrial protein synthesis in sporulation: effects of erythromycin on macromolecular synthesis, meiosis, and ascospore formation in Saccharomyces cerevisiae

Marmiroli, Nelson; Ferri, Maura; Puglisi, P. P.

doi:10.1128/jb.154.1.118-129.1983

Cited by 25 publications

(11 citation statements)

References 29 publications

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“…It was also reported that ASA inhibits b-oxidation (Glasgow et al, 1999;Deva et al, 2001). Strikingly, ascosporogenesis in Saccharomyces cerevisiae occurs only in the presence of mitochondrial respiration (Marmiroli et al, 1983). Consequently, it is now important to determine whether this is also true in Dipodascus geniculatus and other yeasts.…”

Section: Discussionmentioning

confidence: 99%

The release of elongated, sheathed ascospores from bottle-shaped asci inDipodascus geniculatus

Heerden

Wyk

Botes

et al. 2007

FEMS Yeast Research

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Yeasts use different mechanisms to release ascospores of different lengths from bottle-shaped asci. Round to oval-shaped ascospores are enveloped in oxylipin-coated compressible sheaths, enabling ascospores to slide past each other when they reach the narrowing ascus neck. However, more elongated ascospores do not contain sheaths, but are linked by means of oxylipin-coated interlocked hooked ridges on the surfaces of neighboring ascospores, thereby keeping them aligned while they are pushed towards the ascus tip by turgor pressure. In this study, we found elongated, oxylipin-coated sheathed ascospores in Dipodascus geniculatus that are released effectively from bottle-shaped asci without alignment. This is possible because the ascus neck and opening have a diameter that is the same as the length of the ascospore, thus allowing the ascospores to turn sideways without blocking the ascus when they are released. We found that increased concentrations of acetylsalicylic acid inhibit both ascospore release and 3-hydroxy oxylipin production in this yeast, thereby implicating this oxylipin in sexual reproduction.

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Section: Discussionmentioning

confidence: 99%

The release of elongated, sheathed ascospores from bottle-shaped asci inDipodascus geniculatus

Heerden

Wyk

Botes

et al. 2007

FEMS Yeast Research

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“…In addition to the above, aspirin may also inhibit mitochondrial activity by uncoupling mitochondrial oxidative phosphorylation and/or inhibiting electron transport (Somasundaram et al 1997;Norman et al 2004). It is therefore not surprising that the yeast sexual cycle, which has previously been found to be dependent on mitochondrial activity (Marmiroli et al, 1983;Codon et al, 1995), is more susceptible to aspirin than are asexual vegetative cells (Kock et al, 2003). This is particularly true for Ascoidea, Dipodascopsis, Dipodascus and Eremothecium, in which high mitochondrial activity is presumably necessary to produce sufficient energy through aerobic respiration to sustain high production and assembly throughput during the formation of numerous ascospores within a single enlarged sexual cell.…”

Section: Functions Of 3-oh Oxylipinsmentioning

confidence: 99%

Oxylipin studies expose aspirin as antifungal

Kock

Sebolai

Pohl

et al. 2007

FEMS Yeast Research

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The presence of aspirin-sensitive 3-hydroxy fatty acids (i.e. 3-OH oxylipins) in yeasts was first reported in the early 1990s. Since then, these oxidized fatty acids have been found to be widely distributed in yeasts. 3-OH oxylipins may: (1) have potent biological activity in mammalian cells; (2) act as antifungals; and (3) assist during forced spore release from enclosed sexual cells (asci). A link between 3-OH oxylipin production, mitochondria and aspirin sensitivity exists. Research suggests that: (1) 3-OH oxylipins in some yeasts are probably also produced by mitochondria through incomplete beta-oxidation; (2) aspirin inhibits mitochondrial beta-oxidation and 3-OH oxylipin production; (3) yeast sexual stages, which are probably more dependent on mitochondrial activity, are also characterized by higher 3-OH oxylipin levels as compared to asexual stages; (4) yeast sexual developmental stages as well as cell adherence/flocculation are more sensitive to aspirin than corresponding asexual growth stages; and (5) mitochondrion-dependent asexual yeast cells with a strict aerobic metabolism are more sensitive to aspirin than those that can also produce energy through an alternative anaerobic glycolytic fermentative pathway in which mitochondria are not involved. This review interprets a wide network of studies that reveal aspirin to be a novel antifungal.

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“…compared 2‐DE patterns of yeast in sporulation medium with the patterns of vegetatively growing cells; 11 proteins were induced in sporulation medium and 9 proteins specific to vegetative growth were visualised 19. Subsequent work confirmed a novel pattern of protein synthesis during sporulation and also demonstrated that mitochondrial protein synthesis is necessary for the expression of some sporulation‐specific polypeptides and for the sporulation process to occur 5, 17, 20. Although the 2‐DE approach permitted a global proteomic analysis, this early work did not investigate the temporal patterns of protein expression levels and identification of the differentially expressed gel features was not undertaken.…”

Section: Introductionmentioning

confidence: 96%

“…Early studies indicated that protein synthesis is required for initiation and completion of spore formation and also necessary for certain events which are restricted to the sporulation process 15–17. Cytological observation of structures unique to sporulating cells suggested that specialised meiotic proteins might exist 18.…”

Section: Introductionmentioning

confidence: 99%

2‐D DIGE analysis of the budding yeast pH 6–11 proteome in meiosis

et al. 2010

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Meiosis is the cell division that generates haploid gametes from diploid precursors. To provide insight into the functional proteome of budding yeast during meiosis, a 2-D DIGE kinetic approach was used to study proteins in the pH 6-11 range. Nearly 600 protein spots were visualised and 79 spots exhibited statistically significant changes in abundance as cells progressed through meiosis. Expression changes of up to 41-fold were detected and protein sequence information was obtained for 48 spots. Single protein identifications were obtained for 21 spots including different gel mobility forms of 5 proteins. A large number of post-translational events are suggested for these proteins, including processing, modification and import. The data are incorporated into an online 2-DE map of meiotic proteins in budding yeast, which extends our initial DIGE investigation of proteins in the pH 4-7 range. Together, the analyses provide peptide sequence data for 84 protein spots, including 50 single-protein identifications and gel mobility isoforms of 8 proteins. The largest classes of identified proteins include carbon metabolism, protein catabolism, protein folding, protein synthesis and the oxidative stress response. A number of the corresponding genes are required for yeast meiosis and recent studies have identified similar classes of proteins expressed during mammalian meiosis. This proteomic investigation and the resulting protein reference map make an important contribution towards a more detailed molecular view of yeast meiosis.

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Involvement of mitochondrial protein synthesis in sporulation: effects of erythromycin on macromolecular synthesis, meiosis, and ascospore formation in Saccharomyces cerevisiae

Abstract: MATERIALS AND METHODS Strains. The relevant genotype of S. cerevisiae Z270 and the map distances are shown in Fig. 1. This strain was kindly supplied by M. S. Esposito, Donner Laboratory, University of California. Erythromycin-resistant derivatives of strain Z270 were isolated as colo-118

Cited by 25 publications

References 29 publications

The release of elongated, sheathed ascospores from bottle-shaped asci inDipodascus geniculatus

The release of elongated, sheathed ascospores from bottle-shaped asci inDipodascus geniculatus

Oxylipin studies expose aspirin as antifungal

2‐D DIGE analysis of the budding yeast pH 6–11 proteome in meiosis

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