Involvement of myosin VI immunoanalog in pinocytosis and phagocytosis in <i>Amoeba proteus</i>

Sobczak, Magdalena; Wasik, A.; Kłopocka, Wanda; Rędowicz, Maria Jolanta

doi:10.1139/o08-142

Cited by 4 publications

(2 citation statements)

References 35 publications

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“…Most of them revealed the presence of this protein in the regions near the plasma and intracellular membrane structures such as Golgi complex, recycling endosome, and various vesicles involved in endocytosis and secretion or occurring in the active synaptic zone (Hasson et al 1997;Buss et al 2001;Warner et al 2003;Rzadzinska et al 2004;Morriswood et al 2007;Sobczak et al 2008;Roux et al 2009;Puri et al 2010). In these post-embedding experiments, the efficiency of labeling was satisfactory, but actin structures were not visible.…”

Section: Discussionmentioning

confidence: 99%

A pre-embedding immunogold approach reveals localization of myosin VI at the ultrastructural level in the actin cones that mediate Drosophila spermatid individualization

2011

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Stable actin structures play important roles in the development and specialization of differentiated cells. How these structures form, are organized, and are used to mediate physiological processes is not well understood in most cases. In Drosophila testis, stable actin structures, called actin cones, mediate spermatid individualization, a large-scale cellular remodeling process. These actin cones are composed of two structural domains, a front meshwork and a rear region of parallel bundles. Myosin VI is an important player in proper actin cone organization and function. Myosin VI localizes to the cones' fronts and its specific localization is required for proper actin cone formation and function during individualization. To understand how these structures are organized and assembled, ultrastructural studies are important to reveal both organization of actin and the precise localization of actin regulators relative to regions with different filament organizations. In the present work, we have developed a novel pre-embedding immunogold-silver labeling method for high-resolution analysis of protein distribution in actin structures which allowed both satisfactory antibody labeling and good ultrastructural preservation. Electron microscopic studies revealed that myosin VI accumulated at the extreme leading edge of the actin cone and preferentially localized throughout the front meshwork of the cone where branched actin filaments were most concentrated. No myosin VI labeling was found adjacent to the membranes along the length of the cone or connecting neighboring cones. This method has potential to reveal important information about precise relationships between actin-binding proteins, membranes, and different types of actin structures.

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Section: Discussionmentioning

confidence: 99%

A pre-embedding immunogold approach reveals localization of myosin VI at the ultrastructural level in the actin cones that mediate Drosophila spermatid individualization

2011

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“…A. proteus capture prey by phagocytosis, encircling prey with one or more pseudopodia and digesting them in food vacuoles (e.g. described in Jeon & Bell, 1962; Jeon & Jeon, 1976; Kepner & Taliaferro, 1913; Kepner & Whitlock, 1921; Lancaster et al, 2019; Prusch & Britton, 1987; Salt, 1968; Sobczak et al, 2008; Swanson & Baer, 1995). Both mechanical and chemical stimuli produced by prey can induce A. proteus to extend pseudopodia (e.g.…”

Section: Introductionmentioning

confidence: 99%

Formation of multicellular colonies by choanoflagellates increases susceptibility to capture by amoeboid predators

Chin

O'Toole

et al. 2023

J Eukaryotic Microbiology

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Many heterotrophic microbial eukaryotes are size-selective feeders. Some microorganisms increase their size by forming multicellular colonies. We used choanoflagellates, Salpingoeca helianthica, which can be unicellular or form multicellular colonies, to study the effects of multicellularity on vulnerability to predation by the raptorial protozoan predator, Amoeba proteus, which captures prey with pseudopodia. Videomicrography used to measure the behavior of interacting S. helianthica and A. proteus revealed that large choanoflagellate colonies were more susceptible to capture than were small colonies or single cells. Swimming colonies produced larger flow fields than did swimming unicellular choanoflagellates, and the distance of S. helianthica from A. proteus when pseudopod formation started was greater for colonies than for single cells. Prey size did not affect the number of pseudopodia formed and the time between their formation, pulsatile kinematics and speed of extension by pseudopodia, or percent of prey lost by the predator. S. helianthica did not change swimming speed or execute escape maneuvers in response to being pursued by pseudopodia, so size-selective feeding by A. proteus was due to predator behavior rather than prey escape. Our results do not support the theory that the selective advantage of becoming multicellular by choanoflagellate-like ancestors of animals was reduced susceptibility to protozoan predation.

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A novel mutation in the DNM2 gene impairs dynamin 2 localization in skeletal muscle of a patient with late onset centronuclear myopathy

Kierdaszuk

Berdyński

Karolczak

et al. 2013

Neuromuscular Disorders

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Involvement of myosin VI immunoanalog in pinocytosis and phagocytosis in Amoeba proteus

Cited by 4 publications

References 35 publications

A pre-embedding immunogold approach reveals localization of myosin VI at the ultrastructural level in the actin cones that mediate Drosophila spermatid individualization

A pre-embedding immunogold approach reveals localization of myosin VI at the ultrastructural level in the actin cones that mediate Drosophila spermatid individualization

Formation of multicellular colonies by choanoflagellates increases susceptibility to capture by amoeboid predators

A novel mutation in the DNM2 gene impairs dynamin 2 localization in skeletal muscle of a patient with late onset centronuclear myopathy

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