Involvement of NADPH oxidase in high-dose phenolic acid-induced pro-oxidant activity on rat mesenteric venules

Du, Wen-Yuan; Xiao, Ying; Yao, Jian‑Jing; Hao, Zhe; Zhao, Ya-Shuo

doi:10.3892/etm.2016.3923

Cited by 15 publications

(9 citation statements)

References 23 publications

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“…Interestingly, rooster semen supplementation with 50 mM trans-FA, not only did not provide adequate protection, but also showed deleterious effects on motility of spermatozoa and the measured metabolites within the spermatozoa-diluent homogenate, as shown by MDA elevation and TAC reduction levels. This finding is consistent with the results of previous studies that noted high concentrations of phenolic compounds act like a pro-oxidant, produced free radicals, stimulated oxidative toxicity, and induced nitrosylation of proteins ( Jiang et al, 2000 ; Du et al, 2017 ).…”

Section: Discussionsupporting

confidence: 93%

Ameliorative role of trans-ferulic acid on induced oxidative toxicity of rooster semen by β-cyfluthrin during low temperature liquid storage

et al. 2021

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Current study was designed to evaluate the effects of β-cyfluthrin, as a toxicant substance, and trans -ferulic acid ( trans - FA ), as a protective agent, on different parameters of rooster semen upon liquid storage. For this purpose, semen samples of roosters (Ross 308, n = 10, 32-wk-old) were collected twice a week. Good quality samples (≥70% progressive motion) were diluted, pooled and then divided for the purposes of the study. In the first experiment, motility of spermatozoa was evaluated following exposure to different concentrations of β-cyfluthrin (1, 2.5, 5, 10, 20, 40, and 80 µM) at 0, 24, and 48 h of storage. In the second experiment, constant doses of β-cyfluthrin (10 µM) alone or in combination with trans -FA (10, 25 mM) were assessed on motility and viability of spermatozoa at 0, 24, and 48 h time points. Moreover, amounts of malondialdehyde ( MDA ), total antioxidant capacity ( TAC ), total nitrate-nitrite, total hydroperoxide ( HPO ), and activity of superoxide dismutase ( SOD ) were evaluated in the homogenate of spermatozoa-diluent at studied time points. Results of the first experiment showed that amounts of β-cyfluthrin greater than 5 µM, significantly reduced the motility of spermatozoa at 24 and 48 h of storage ( P < 0.05). The second experiment demonstrated that, trans -FA especially at 10, 25 mM doses restored the motility and viability of spermatozoa compared to β-cyfluthrin treated group ( P < 0.05). Amounts of MDA (10, 25 mM), hydroperoxide (10, 25, and 50 mM), and nitrate-nitrite (10, 25, and 50 mM) were lower and TAC (10 and 25 mM) were greater in trans -FA + β-cyfluthrin treated groups compared to β-cyfluthrin alone treated samples ( P < 0.05). However, activity of SOD did not show significant changes by the treatment ( P > 0.05). It seems that trans -FA could ameliorate toxic effect of β-cyfluthrin via reduction of peroxidative (as evident by measurement of MDA) and nitrosative (as evident by measurement of nitrate-nitrite) reactions over cold preservation of rooster semen.

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Section: Discussionsupporting

confidence: 93%

Ameliorative role of trans-ferulic acid on induced oxidative toxicity of rooster semen by β-cyfluthrin during low temperature liquid storage

et al. 2021

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“…At the same time, there is rather convincing evidence that PhCs can exhibit pro-oxidant effects (Figure ). The dual anti/pro-oxidant behavior of this kind of compounds seems to be concentration-dependent − and modulated by environmental factors, such as pH and the presence of transition metals. ,− Antioxidants are, by definition, good reductant chemical agents, and PhCs are not the exception. Thus, there is a viable hypothesis that their pro-oxidant behavior arises from their ability to reduce metal ions, particularly Cu(II), making Cu(I) available to be involved in Fenton-like reactions, i.e., promoting the formation of ROS, in particular the very reactive and harmful hydroxyl radical ( • OH). ,, This, however, does not rule out the fact that other chemical processes might be associated with the pro-oxidant behavior of PhCs.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Investigation of the Antioxidant and Pro-oxidant Effects of Phenolic Compounds: A Double-Edged Sword in the Context of Oxidative Stress?

et al. 2018

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Oxidative stress (OS) is a health-threatening process that is involved, at least partially, in the development of several diseases. Although antioxidants can be used as a chemical defense against OS, they might also exhibit pro-oxidant effects, depending on environmental conditions. In this work, such a dual behavior was investigated for phenolic compounds (PhCs) within the framework of the density functional theory and based on kinetic data. Multiple reaction mechanisms were considered in both cases. The presence of redox metals, the pH, and the possibility that PhCs might be transformed into benzoquinones were identified as key aspects in the antioxidant versus pro-oxidant effects of these compounds. The main virtues of PhCs as antioxidants are their radical trapping activity, their regeneration under physiological conditions, and their behavior as OH-inactivating ligands. The main risks of PhCs as pro-oxidants are predicted to be the role of phenolate ions in the reduction of metal ions, which can promote Fenton-like reactions, and the formation of benzoquinones that might cause protein arylation at cysteine sites. Although the benefits seem to overcome the hazards, to properly design chemical strategies against OS using PhCs, it is highly recommended to carefully explore their duality in this context.

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“…Bezerra et al (2016) also reported that the hydroethanolic extract of Turnera diffusa, containing flavone-C, O-diglycoside as the main constituents, was found to be toxic at high concentrations, specifically at 1000 µg/mL, evidenced by increased cell death of the astrocyte culture after 6 and 24 hours of incubation. Further, Du et al (2017) reported that intravenous injection of high doses of phenolic acids to male Wistar rats led to an imbalance between oxidant and antioxidant mechanisms, boosting the expression level of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, resulting in damage to microvascular endothelial cells.…”

Section: Discussionmentioning

confidence: 99%

Zebrafish Embryotoxicity and Teratogenic Effects of Christia vespertilionis Leaf Extract

Norazhar¹,

Ibrahim²,

Hodin³

et al. 2022

JTAS

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Christia vespertilionis or butterfly wings is a traditional medicinal plant used to treat, among others, colds and bronchitis. The plant was also reported to be a remedy for cancer, with several products based on the plant becoming commercially available, raising some safety concerns on its consumption. The present study was carried out to assess the toxic and teratogenic effects of the plant on the embryonic development of zebrafish (Danio rerio) as the animal model. Zebrafish embryos were exposed to 50, 100, 200, 400, and 800 μg/mL of the methanolic leaf extract of C. vespertilionis, starting from 5 to 120 hours post-fertilization (hpf). The median lethal concentration (LC50) value of the extract was determined to be 419.84 μg/mL, which is within the safety limit stipulated by the Organisation for Economic Co-operation and Development (OECD) guideline. However, results from the teratogenicity evaluation revealed multiple signs of developmental defects in embryos exposed to 200 μg/mL and higher concentrations of the extract. The magnitude of the defects was observed to be concentration-dependent. Moreover, no hatching and spontaneous movement of tail coiling were observed at 400 and 800 μg/mL concentrations due to the delayed growth and early mortality, respectively. A significant reduction in heartbeat rate was also reported for the surviving embryos at the 400 μg/mL test concentration. The present study has provided preliminary results on the potentially toxic and teratogenic effects of the extract at high concentrations.

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Involvement of NADPH oxidase in high-dose phenolic acid-induced pro-oxidant activity on rat mesenteric venules

Cited by 15 publications

References 23 publications

Ameliorative role of trans-ferulic acid on induced oxidative toxicity of rooster semen by β-cyfluthrin during low temperature liquid storage

Ameliorative role of trans-ferulic acid on induced oxidative toxicity of rooster semen by β-cyfluthrin during low temperature liquid storage

Comprehensive Investigation of the Antioxidant and Pro-oxidant Effects of Phenolic Compounds: A Double-Edged Sword in the Context of Oxidative Stress?

Zebrafish Embryotoxicity and Teratogenic Effects of Christia vespertilionis Leaf Extract

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