Involvement of NF-<i>κ</i>B/IL-6 Pathway in the Processing of Colorectal Carcinogenesis in Colitis Mice

Yang, Hang; Qi, Haili; Ren, Jingli; Cui, Jing; Li, Zhenfeng; Cui, Guanglin

doi:10.1155/2014/130981

Cited by 25 publications

(16 citation statements)

References 40 publications

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“…At the same time, populations that are continuously exposed to As may chronically produce IL-8 as a result of the increases in the NF-κB mRNA levels [27]. The IL-6 and IL-8 mRNA expression by the liver tissue was significantly increased with the stimulus [28][29][30][31]. Our results on these endpoints are not broadly in agreement with our previous measurements of cytokines induced by toxins.…”

Section: Discussionsupporting

confidence: 49%

Arsenic Trioxide Attenuates NF-κB and Cytokine mRNA Levels in the Livers of Cocks

Zhang

Zhao

Guo

et al. 2015

Biol Trace Elem Res

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Arsenic (As) is a trace element widely found in nature. It exists in several forms, including organic arsenic, inorganic arsenic, and trivalent arsenic, the most toxic. Arsenic trioxide (As2O3) is widespread in nature. This form tends to accumulate in animals and humans and therefore has a potential harm for them. Cytokines play essential roles in the immune response and inflammation. Although the importance of cytokines in the responses to arsenic exposure has been demonstrated in many types of mammals, the function of these in poultry, especially in chickens, remains unclear. The purpose of the present study was to examine the effect of As2O3 exposure on cytokines in cock livers. In this study, 72 1-day-old male Hy-line cocks were randomly divided into four groups including the control group, low-As group, middle-As group, and high-As group. The livers were collected on days 30, 60, and 90 of the experiment. The levels of nuclear factor-kappa B (NF-κB), tumor necrosis factor-alpha (TNF-α), interleukin-4 (IL-4), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-12 beta (IL-12β), and interleukin-1 beta (IL-1β) mRNA in the livers of the cocks were measured using real-time PCR. The results showed that the expression levels of IL-6, IL-8, TNF-α, and NF-κB which seemed to be a critical mediator in the inflammatory response tended to increase in the birds chronically treated with As2O3. However, the mRNA expression levels of IL-4, IL-12β, and IL-1β were decreased in the experiment. The information regarding the effects of As2O3 on cytokine mRNA expression generated in this study will be important information for arsenic toxicology evaluation.

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Section: Discussionsupporting

confidence: 49%

Arsenic Trioxide Attenuates NF-κB and Cytokine mRNA Levels in the Livers of Cocks

Zhang

Zhao

Guo

et al. 2015

Biol Trace Elem Res

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“…The abnormal activation of NF-κB contributes to significant cell proliferation and migration in CRC and in other types of cancer ( 3 ), and the inhibition of NF-κB activity has been shown to significantly reduce cancer cell growth and enhance cell apoptosis, indicating that NF-κB members are potential therapeutic targets in tumors ( 4 ). Mammalian NF-κB members mainly include p65 (RelA), p105/p50, RelB, p100/p52 and c-Rel ( 5 ), and two distinct pathways are involved in the regulation of NF-κB activation: the canonical and the non-canonical pathways. The canonical pathway of NF-κB is controlled by the IκB kinase (IKK) complex, which is comprised of IKKα, IKKβ and IKKγ.…”

Section: Introductionmentioning

confidence: 99%

Downregulation of miR-518a-3p activates the NIK-dependent NF-κB pathway in colorectal cancer

Zhao

et al. 2015

International Journal of Molecular Medicine

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The aim of the present study was to investigate the biological role and underlying mechanisms of action of miR-518a-3p in the progression and invasion of colorectal cancer (CRC). Reverse transcription-quantitative PCR (RT-qPCR) was used to examine the mRNA expression levels of miR-518a-3p in 5 CRC cell lines (SW480, SW620, HCT116, HT29 and LoVo) in a normal colonic cell line, NCM460, as well as in tumor tissues with or without metastases. The biological effects of miR-518a-3p were assessed in the CRC cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometric analysis, and RT-qPCR and western blot analyses were employed to evaluate the expression of miR-518a-3p targets. The regulation of NF-κB-inducing kinase (NIK) by miR-518a-3p was confirmed using luciferase activity assays. Our results revealed that miR-518a-3p was significantly downregulated in the CRC cell lines compared with the normal colonic cell line (P<0.05), as well as in the CRC tissues with distant metastases compared with the tissues without metastases. The downregulation of miR-518a-3p was associated with tumor size, distant metastasis and TNM stage in the patients with CRC. Moreover, the ectopic expression of miR-518a-3p and the inhibition of NIK by RNA interference markedly reduced cell proliferation and enhanced the apoptosis of CRC cells. Further experiments revealed that NIK, a regulator of NF-κB, was a downstream target of miR-518a-3p. The presents findings indicate that miR-518a-3p plays an important role in the progression of CRC by targeting NIK.

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“…Previous reports have found DSS increases LCN2 in both plasma and feces [312,314], while LCN2 mRNA was not induced in WT mice treated with paraquat [315]. Similarly we saw increased IL-6 following LPS and paraquat administration, as we and others have previously reported [298,316], however, no effect of DSS was observed on this measure contrary to previous reports in the gut [317]. In addition, we further found no alteration in IL-1B or IL-10 with either DSS or LPS and paraquat treatment.…”

Section: Dss Treatment Increased Intestinal Tnf-α and Il-1β Mrna Exprcontrasting

confidence: 57%

Environmental toxicant exposure and Parkinson’s disease: LRRK2 and inflammatory processes

Dwyer¹

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Parkinson's disease (PD) results from the progressive loss of dopamine producing neurons in the Substantia Nigra pars comapcta (SNc). This loss is thought to occur over several years to decades and current evidence suggests that neuroinflammation may play a central role in this loss. Numerous epidemiological studies have implicated chronic exposure to environmental toxicants such as heavy metals and pesticides to risk of developing PD. Indeed, many of these risk factors have been validated as rodent models of PD, able to induce dopaminergic cell loss in the SNc as well as motor dysfunction. While research strongly supports a role for environmental toxicants and inflammation in PD relatively little work has examined the interactions between these toxicants and other risk factors including senescence, genetic vulnerabilities, the gut microbiota or iii This thesis is based upon the following four manuscripts referred to in text by their corresponding chapter numbers.

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Involvement of NF-κB/IL-6 Pathway in the Processing of Colorectal Carcinogenesis in Colitis Mice

Cited by 25 publications

References 40 publications

Arsenic Trioxide Attenuates NF-κB and Cytokine mRNA Levels in the Livers of Cocks

Arsenic Trioxide Attenuates NF-κB and Cytokine mRNA Levels in the Livers of Cocks

Downregulation of miR-518a-3p activates the NIK-dependent NF-κB pathway in colorectal cancer

Environmental toxicant exposure and Parkinson’s disease: LRRK2 and inflammatory processes

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