Involvement of NF-κB-mediated expression of galectin-3-binding protein in TNF-α-induced breast cancer cell adhesion

Noma, Naruto; Simizu, Siro; Kambayashi, Yusuke; Kabe, Yoshio; Suematsu, Makoto; Umezawa, Kazuo

doi:10.3892/or.2012.1733

Cited by 15 publications

(11 citation statements)

References 24 publications

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“…Gal-3 has been considered a tumor-associated antigen of malignancy level in breast cancer, and is implicated in aspects, such as angiogenesis [219], adhesion [220], invasion [221], apoptosis inhibition [222], migration [223], and therapeutic resistance [224]. Chen et al, through hydrazide-based chemistry method combined with liquid chromatography tandem-mass spectrometry (LC-MS/MS), identified 72 unique N-linked glycoproteins that were significantly regulated in triple negative breast cancers, among which is LGALS3BP.…”

Section: Hypoxia Alters Cell Location Of Galectins and Interaction Wimentioning

confidence: 99%

Glycobiology Modifications in Intratumoral Hypoxia: The Breathless Side of Glycans Interaction

Silva-filho¹,

Sena²,

Lima

et al. 2017

Cell Physiol Biochem

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Post-translational and co-translational enzymatic addition of glycans (glycosylation) to proteins, lipids, and other carbohydrates, is a powerful regulator of the molecular machinery involved in cell cycle, adhesion, invasion, and signal transduction, and is usually seen in both in vivo and in vitro cancer models. Glycosyltransferases can alter the glycosylation pattern of normal cells, subsequently leading to the establishment and progression of several diseases, including cancer. Furthermore, a growing amount of research has shown that different oxygen tensions, mainly hypoxia, leads to a markedly altered glycosylation, resulting in altered glycan-receptor interactions. Alteration of intracellular glucose metabolism, from aerobic cellular respiration to anaerobic glycolysis, inhibition of integrin 3α1β translocation to the plasma membrane, decreased 1,2-fucosylation of cell-surface glycans, and galectin overexpression are some consequences of the hypoxic tumor microenvironment. Additionally, increased expression of gangliosides carrying N-glycolyl sialic acid can also be significantly affected by hypoxia. For all these reasons, it is possible to realize that hypoxia strongly alters glycobiologic events within tumors, leading to changes in their behavior. This review aims to analyze the complexity and importance of glycoconjugates and their molecular interaction network in the hypoxic context of many solid tumors.

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Section: Hypoxia Alters Cell Location Of Galectins and Interaction Wimentioning

confidence: 99%

Glycobiology Modifications in Intratumoral Hypoxia: The Breathless Side of Glycans Interaction

Silva-filho¹,

Sena²,

Lima

et al. 2017

Cell Physiol Biochem

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“…(1) Galectin-3-binding protein (LG3BP) was reported to be a candidate diagnostic biomarker for large-cell neuroendocrine lung carcinoma 26 . In addition, galectin-3-binding protein was also reported to induce galectin-mediated tumor cell aggregation to increase the survival of cancer cells in the bloodstream during the metastatic process 27,28 . (2) Alpha-1-acid glycoprotein (A1AG) was reported as potential lung cancer serum biomarker 29 .…”

Section: Resultsmentioning

confidence: 99%

Early urine proteome changes in the Walker-256 tail-vein injection rat model

Wei

Meng

et al. 2019

Sci Rep

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Detection of cancer at its early stage is important for treatment. Urine, which is not regulated by homeostatic mechanisms, reflects early systemic changes throughout the whole body and can be used for the early detection of cancer. In this study, the Walker-256 tail-vein injection rat model was established to find whether the urine proteome could reflect early changes if tumor grown in lung. Urine samples from the control group (n = 7) and Walker-256 tail-vein injection group (n = 7) on days 2, 4, 6 and 9 were analyzed by label-free proteomic quantitative methods. On day 2, when lung tumor nodules did not appear, 62 differential proteins were identified. They were associated with epithelial cell differentiation, regulation of immune system processes and the classical complement activation pathway. On day 4, when lung tumor nodules appeared, 72 differential proteins were identified. They were associated with the innate immune response and positive regulation of phagocytosis. On day 6, when body weight began to decrease, 117 differential proteins were identified. On day 9, the identified 125 differential proteins were associated with the B cell receptor signaling pathway and the positive regulation of B cell activation. Our results indicate that (1) the urine proteome changed even on the second day after tail-vein injection of Walker-256 cells and that (2) compared to previous studies, the urine proteomes were different when the same cancer cells were grown in different organs.

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“…It is a binding partner of Gal-1 and Gal-3, which promote integrin-mediated cell adhesion, and significantly elevated expression of Gal-3 BP in the serum or tumor tissues is associated with poor prognosis in a variety of malignancies including breast cancer (29), lung cancer (30), and PC (31). Additionally, Gal-3 BP is involved in the promotion of integrinmediated tumor cell adhesion to the ECM proteins in colon cancer (32), breast cancer (33), and the formation of metastasis in lung cancer (34). Our study reveals that targeting Gal-3 BP may prevent the stimulatory effect of SCs in PC cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Schwann Cell Stimulation of Pancreatic Cancer Cells: A Proteomic Analysis

Ferdoushi

Griffin

et al. 2020

Front. Oncol.

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Schwann cells (SCs), the glial component of peripheral nerves, have been identified as promoters of pancreatic cancer (PC) progression, but the molecular mechanisms are unclear. In the present study, we aimed to identify proteins released by SCs that could stimulate PC growth and invasion. Proteomic analysis of human primary SC secretome was performed using liquid chromatography-tandem mass spectrometry, and a total of 13,796 unique peptides corresponding to 1,470 individual proteins were identified. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment were conducted using the Database for Annotation, Visualization, and Integrated Discovery. Metabolic and cell-cell adhesion pathways showed the highest levels of enrichment, a finding in line with the supportive role of SCs in peripheral nerves. We identified seven SC-secreted proteins that were validated by western blot. The involvement of these SC-secreted proteins was further demonstrated by using blocking antibodies. PC cell proliferation and invasion induced by SC-conditioned media were decreased using blocking antibodies against the matrix metalloproteinase-2, cathepsin D, plasminogen activator inhibitor-1, and galectin-1. Blocking antibodies against the proteoglycan biglycan, galectin-3 binding protein, and tissue inhibitor of metalloproteinases-2 decreased only the proliferation but not the invasion of PC cells. Together, this study delineates the secretome of human SCs and identifies proteins that can stimulate PC cell growth and invasion and therefore constitute potential therapeutic targets.

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Involvement of NF-κB-mediated expression of galectin-3-binding protein in TNF-α-induced breast cancer cell adhesion

Cited by 15 publications

References 24 publications

Glycobiology Modifications in Intratumoral Hypoxia: The Breathless Side of Glycans Interaction

Glycobiology Modifications in Intratumoral Hypoxia: The Breathless Side of Glycans Interaction

Early urine proteome changes in the Walker-256 tail-vein injection rat model

Schwann Cell Stimulation of Pancreatic Cancer Cells: A Proteomic Analysis

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