Involvement of nitric oxide in anticompulsive-like effect of agmatine on marble-burying behaviour in mice

Gawali, Nitin B.; Chowdhury, Amrita A.; Kothavade, Pankaj S.; Bulani, Vipin D.; Nagmoti, Dnyaneshwar M.; Juvekar, Archana R.

doi:10.1016/j.ejphar.2015.11.044

Cited by 22 publications

(19 citation statements)

References 55 publications

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“…The procedure used for MB followed the paradigm used commonly in the literature (Deacon 2006, Gawali et al 2016, Witkin 2008, except that we conducted the tests in the home cages rather than in novel cages. Logic was to mimic human OCD in which compulsive behaviors occur with unsettling disturbances of a familiar environment.…”

Section: Methodsmentioning

confidence: 99%

“…Indeed, MB has also appeared in the literature as a measure for autism, motivation or general anxiety disorder (GAD) (Ene et al 2016, Jury et al 2015, Silverman et al 2015. Despite the criticisms, MB continues to appear regularly in the literature as a measure of compulsion (Gawali et al 2016, Kudryashov et al 2016, Nichols et al 2016, Satta et al 2016.…”

Section: Introductionmentioning

confidence: 99%

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Marble burying as compulsive behaviors in male and female mice

Taylor¹,

Lerch²,

Chourbaji³

2017

Acta Neurobiologiae Experimentalis

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Marble burying is considered an, albeit controversial, animal model of the compulsive like behaviors of obsessive-compulsive disorder (OCD). Hallmark features of OCD patients are similarities and, more prominent, differences from anxiety disorders, e.g., the absence of sex differences and resistance to spontaneous remission. We report an experiment on marble burying by male and female C57/BL6/N mice. Animals were administered either the classic anxiolytic drug, diazepam, that targets the GABA receptor or a "pure" inhibitor of the serotonin transporter, escitalopram, that has been reported to be particularly effective in OCD. A burying paradigm that more precisely mimics the human condition was used, e.g., testing in the home environment, chronic drug exposure and acknowledging individual differences by pre-selecting for high marble burying. Results were that there were no sex differences in groups treated with drugs or in control mice. Both diazepam and escitalopram decreased numbers of marbles buried compared to vehicle-only controls in the absence of correlated changes in anxiety. Diazepam, however, was more effective than escitalopram in suppressing MB. The conclusion is that along with serotonin, GABA is involved in regulating compulsive behaviors. The marble burying paradigm may prove more useful for pharmacological drugs tests of impulsivity or attention deficit because of the involvement of serotonin and GABA in both disorders.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Marble burying as compulsive behaviors in male and female mice

Taylor¹,

Lerch²,

Chourbaji³

2017

Acta Neurobiologiae Experimentalis

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“…Although it is not uncommon to apply a specific behavioral measurement in the analysis of behaviors that are believed to mimic different mental disorders-for example, the open field test, in assessments of anxiety (Prut & Belzung, 2003), aggression (Lewis, Gariépy, Gendreau, Nichols, & Mailman, 1994), and social interaction (File & Seth, 2003)-the use of the MBT differs from these tests in that, given the different approaches taken to its application, no clear hypothesis seems to underlie its use within the conceptual and contextual boundaries of a specific disorder. In fact, the realities that the test is applied as a screening tool for specific conditions and drug responses without methodological congruence between laboratories (Dey, Chatterjee, & Kumar, 2016;Gawali et al, 2016) and that it demonstrates inconsistent responses to different treatments (Li, Morrow, & Witkin, 2006;Njung'e & Handley, 1991a) complicate comparisons of the published findings and cloud the translational usefulness of marble-burying as a measure of anxiolytic or anticompulsive drug action.…”

Section: Introductionmentioning

confidence: 99%

A critical inquiry into marble-burying as a preclinical screening paradigm of relevance for anxiety and obsessive–compulsive disorder: Mapping the way forward

Brouwer

Fick

Harvey

et al. 2018

Cogn Affect Behav Neurosci

125

111

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Rodent marble-burying behavior in the marble-burying test (MBT) is employed as a model or measure to study anxiety-and compulsive-like behaviors or anxiolytic and anticompulsive drug action. However, the test responds variably to a range of pharmacological interventions, and little consensus exists regarding specific methodologies for its execution. Regardless, the test is widely applied to investigate the effects of pharmacological, genetic, and behavioral manipulations on purported behaviors related to the said neuropsychiatric constructs. Therefore, in the present review we attempt to expound the collective translational significance of the MBT. We do this by (1) reviewing burying behavior as a natural behavioral phenotype, (2) highlighting key aspects of anxiety and obsessive-compulsive disorder from a translational perspective, (3) reviewing the history and proof of concept of the MBT, (4) critically appraising potential methodological confounds in execution of the MBT, and (5) dissecting responses of the MBT to various pharmacological interventions. We conclude by underlining that the collective translational value of the MBT will be strengthened by contextually valid experimental designs and objective reporting of data.

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“…Contradictory results were already observed in previous studies using L‐arginine or other NO increasing drugs (e.g., NO donor) in different animal models of anxiety, and both anxiolytic‐like (e.g., Kalouda & Pitsikas, ; Spiacci, Kanamaru, Guimarães, & Oliveira, ) and anxiogenic‐like effects were found (e.g., Umathe et al, ). However, despite these contradictory results of L‐arginine in animal models of anxiety, L‐arginine challenge had been successfully used to detect a nitrergic mediation of anxiolytic‐like effect of treatments in different conditions (e.g., Bonassoli, Contardi, Milani, & De Oliveira, ; Siba et al, ), including marble‐burying behavior (Gawali et al, ; Krass et al, ; Umathe et al, ). Thus, for the first time, the present results indicated an important role of NO transmission on anxiolytic‐like effect of C. sinensis essential oil.…”

Section: Discussionmentioning

confidence: 99%

The nitrergic neurotransmission contributes to the anxiolytic‐like effect of Citrus sinensis essential oil in animal models

Hocayen

Wendler

Vecchia

et al. 2019

Phytotherapy Research

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Citrus fragrances have been used in aromatherapy for the treatment of anxiety, and the essential oil of Citrus sinensis (sweet orange) has shown promising results, although its mechanism of action was not known. The objective of this study was to evaluate the involvement of nitric oxide (NO) neurotransmission in the anxiolytic-like effect of C. sinensis essential oil. Swiss male mice were submitted to 15 min of C. sinensis essential oil inhalation (1%, 2.5%, 5%, and 10%) and tested in the marble-burying test, neophobia-induced hypophagia, and light/dark test. Locomotor activity was evaluated in an automated locomotor activity box. The coadministration of C. sinensis essential oil with L-arginine (200 mg/kg, i.p.), an NO precursor, was used for the behavioral evaluation of nitrergic system mediation. Additionally, the NO synthase activity was measured by nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) analysis in the cerebral cortex. C. sinensis essential oil exerted anxiolytic-like effect at dose that did not change locomotor activity. Moreover, L-arginine pretreatment prevented this anxiolytic-like effect on marble-burying test. Finally, C. sinensis essential oil reduced the NADPH-d positive cells. Thus, the nitrergic neurotransmission plays a relevant role in the anxiolytic-like effect C. sinensis essential oil.

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Involvement of nitric oxide in anticompulsive-like effect of agmatine on marble-burying behaviour in mice

Cited by 22 publications

References 55 publications

Marble burying as compulsive behaviors in male and female mice

Marble burying as compulsive behaviors in male and female mice

A critical inquiry into marble-burying as a preclinical screening paradigm of relevance for anxiety and obsessive–compulsive disorder: Mapping the way forward

The nitrergic neurotransmission contributes to the anxiolytic‐like effect of Citrus sinensis essential oil in animal models

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