Involvement of NPY Y2 receptor subtype in the control of the spontaneous NO/GnRH release at the rat median eminence

Knauf, Claude; Chuoï, Myriam Mariot Tran Van; Jirou-Najou, Jean-Luc; Mortreux, Geneviève; Beauvillain, Jean-Claudé; Croix, Dominique

doi:10.1097/00001756-200110290-00043

Cited by 6 publications

(2 citation statements)

References 23 publications

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“…We suggest from our study that NPY may be a marker for null-cell adenomas. This conclusion is similar to that of Grouzmann et al [9] Our data showed that the NPY expression level in prolactinomas was obviously lower than that of other subtypes of pituitary adenomas. As a neuroendocrine peptide, NPY can be considered to be a signal amplifier that plays different roles in modulating physiological functions.…”

Section: Discussionsupporting

confidence: 93%

Expression of neuropeptide Y in human pituitary adenoma

Chen

Zheng

et al. 2006

Chin. J. Clin. Oncol.

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OBJECTIVE Neuropeptide Y (NPY) acts as a neuroendocrine modulator in the anterior pituitary, and NPY mRNA and NPY-immunoreactivity have been detected in normal human anterior pituitaries. However, only a few studies of NPY expression in human pituitary adenomas have been published. Our study was conducted to determine whether or not adenomatous cells express NPY, to investigate the relationship between NPY expression and the subtypes of pituitary adenoma and to explore the clinical significance of NP¥. METHODS The study included tissues from 58 patients with pituitary adenomas who underwent surgery because of their clinical diagnosis. Using'a highly specific anti-NPY polyclonal antibody, immunohistochemical analysis was performed on the surgically removed pituitary adenomas. Six fresh specimens also were examined using immuno-electron microscopy. NPY was labeled with colloidal gold in order to study the distribution of NPY at the subcellular level. RESULTS The NPY expression level was significantly different among subgroups of pituitary adenomas (P<0.05). NPY was immuno-detected in 58.6% of all adenomas, in 91.7% of gonadotrophic adenomas and in 14.3% of prolactinomas. NPY expression was slightly lower in invasive pituitary adenomas compared to noninvasive adenomas, but the difference was not significant (t=1.81, P>0.05). Of particular interest was the finding that vascular endothelial cells showed positive NPY expression in some pituitary adenomas. Parts of strongly positive tumor cells were seen in channels formed without endothelial cells, but which contained some red blood cells in a formation similar to so-called vasculogenic mimicry. Immuno-electron microscopy demonstrated that 4 of the 6 fresh specimens displayed positive NPY staining with a high density of gold particles located mainly in the secretory granulas. In addition, gold particles were sparsely detected in the rough endoplasmic reticulum and cell matrix. CONCLUSION NPY exists in pituitary adenomas and its expression level was related to the types and biological characteristics of the pituitary adenomas. NPY may have a depressive effect on tumor cellular proliferation in pituitary adenomas. NPY possibly participates in modulating angiogenesis and hemodynamic changes in pituitary adenomas. KEYWORDS: neuropeplide Y, pituitary adenoma, immunohistochemistry, immuno-eleclron microscopy.europeptide Y (NPY) is a widely distributed peptide in the brain, N with its highest concentration found in some hypothalamic nuclei. Cll NPY may function as a neuroendocrine modulator in the anterior pituitary. NPY mRNA and NPY-immunoreactivity have been detected in the normal human anterior pituitary gland and the NPY Y2 receptor subtype has been shown to be present in the neural lobe. E2j However, studies on the expression of NPY in human pituitary adenomas are rare. Our study was conducted to determine whether or not

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Section: Discussionsupporting

confidence: 93%

Expression of neuropeptide Y in human pituitary adenoma

Chen

Zheng

et al. 2006

Chin. J. Clin. Oncol.

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“…NO plays an important role in the female reproductive system and in the release of Gonadotropin-releasing hormone (GnRH) (Knauf et al. 2001). GnRH may stimulate the pituitary gland to release follicle-stimulating hormone and luteinizing hormone, which together, can promote the growth and development of ovarian follicles.…”

Section: Discussionmentioning

confidence: 99%

¹H NMR plasma metabolomic profiling of ovarian quiescence in energy balanced postpartum dairy cows

Zhang

Wang

Bai

et al. 2018

Veterinary Quarterly

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As the milk production of dairy cows increases, the reproductive capacity gradually declines. Ovarian quiescence has become one of the concerns of the dairy industry. Objective: To explore the different plasma metabolite levels between estrus and anestrus in energy balanced (EB) dairy cows. Methods: Ten estrous and 10 anestrus EB Holstein cows in early lactation were selected for the study.1 H nuclear magnetic resonance technology was used to detect plasma metabolites and screen different plasma metabolites between anestrous and estrous cows at 60-90 days postpartum using multivariate statistical analysis. Results: Within an elective waiting period of 60À90 days postpartum mean plasma concentration of total estrogens was significantly higher in estrus cows as compared to anestrus cows (71.2 ± 26.0 and 42.4 ± 16.7 pg/mL, respectively). Seven plasma metabolites (isoleucine, leucine, valine, alanine, arginine, choline and phosphatecholine) demonstrated significant decreases in estrous dairy cows relative to anestrous subjects. The main pathway was leucine, isoleucine and valine biosynthesis. Conclusion: Anestrus in dairy cows is accompanied by alterations in amino acid, glucose and lipid metabolism based on 1 H NMR analysis.

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Neuropeptide Y induces gonadotropin-releasing hormone gene expression directly and through conditioned medium from mHypoE-38 NPY neurons

Dhillon¹,

Gingerich²,

Belsham³

2009

Regulatory Peptides

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Involvement of NPY Y2 receptor subtype in the control of the spontaneous NO/GnRH release at the rat median eminence

Cited by 6 publications

References 23 publications

Expression of neuropeptide Y in human pituitary adenoma

Expression of neuropeptide Y in human pituitary adenoma

¹H NMR plasma metabolomic profiling of ovarian quiescence in energy balanced postpartum dairy cows

Neuropeptide Y induces gonadotropin-releasing hormone gene expression directly and through conditioned medium from mHypoE-38 NPY neurons

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Involvement of NPY Y2 receptor subtype in the control of the spontaneous NO/GnRH release at the rat median eminence

Cited by 6 publications

References 23 publications

Expression of neuropeptide Y in human pituitary adenoma

Expression of neuropeptide Y in human pituitary adenoma

1H NMR plasma metabolomic profiling of ovarian quiescence in energy balanced postpartum dairy cows

Neuropeptide Y induces gonadotropin-releasing hormone gene expression directly and through conditioned medium from mHypoE-38 NPY neurons

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Product

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¹H NMR plasma metabolomic profiling of ovarian quiescence in energy balanced postpartum dairy cows