Involvement of obestatin, cyclin-dependent kinase and protein kinase C in control of feline ovarian cell viability and hormones release

Loncová, Barbora; Fabová, Zuzana; Sirotkin, Alexander V.

doi:10.1016/j.repbio.2021.100560

Cited by 7 publications

(4 citation statements)

References 14 publications

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“…Therefore, the AREG and EREG could promote the S phase of the cell cycle. Our results are consistent with previous findings (Fabová et al., 2022; Loncová, Fabová, & Sirotkin, 2023), which showed similar stimulatory effects of these growth factors on human granulosa cell proliferation, as indicated by the accumulation of PCNA and cyclin B1.…”

Section: Discussionsupporting

confidence: 94%

“…Ovarian granulosa cells were gently scraped from the inner surface of the washed ovarian follicles (800–1200 μm) with a lancet and isolated by centrifugation at 200 g for 10 min as previously described (Loncová, Fabová, & Sirotkin, 2023). Afterwards, the cells were resuspended and cultured in sterile Dulbecco's modified Eagle's medium (DMEM/F12), 1:1 medium (BioWhittaker; Lonza, Verviers, Belgium) supplemented with 10% foetal bovine serum (Bio‐West, Inc., Logan, UT, USA) and 1% antibiotic‐antimycotic solution (Sigma–Aldrich, St. Louis, MO, USA).…”

Section: Methodsmentioning

confidence: 99%

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The growth factors amphiregulin and epiregulin are novel stimulators of feline ovarian granulosa cell functions

Fabová,

Loncová,

Harrath

et al. 2024

Reprod Domestic Animals

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This study aimed to investigate the impact of the epidermal growth factor receptor ligands amphiregulin (AREG) and epiregulin (EREG) on the fundamental functions of feline ovarian granulosa cells. Granulosa cells isolated from feline ovaries were incubated with AREG and EREG (0, 0.1, 1 or 10 ng/mL). The effects of these growth factors on cell viability, proliferation (assessed through BrdU incorporation), nuclear apoptosis (evaluated through nuclear DNA fragmentation) and the release of progesterone and estradiol were determined using Cell Counting Kit‐8 assays, BrdU analysis, TUNEL assays and ELISAs, respectively. Both AREG and EREG increased cell viability, proliferation and steroid hormone release and reduced apoptosis. The present findings suggest that these epidermal growth factor receptor ligands may serve as physiological stimulators of feline ovarian cell functions.

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Section: Discussionsupporting

confidence: 94%

Section: Methodsmentioning

confidence: 99%

The growth factors amphiregulin and epiregulin are novel stimulators of feline ovarian granulosa cell functions

Fabová,

Loncová,

Harrath

et al. 2024

Reprod Domestic Animals

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“…The accuracy of BI–PEG-PSBP NPs in sensing intracellular LDs and HClO was verified by treating the foam cells with calphostin c (Cal), a foaming inhibitor. , On the one hand, Cal could reverse the pathologic progression of the advanced foam cells. As shown in Figure a–c, Cal caused a substantial reduction of LDs, along with a dramatic decrease of the green fluorescence in the B-CF 3 channel and an increase in the red fluorescence intensity.…”

Section: Resultsmentioning

confidence: 99%

“…The accuracy of BI−PEG-PSBP NPs in sensing intracellular LDs and HClO was verified by treating the foam cells with calphostin c (Cal), a foaming inhibitor. 44,45 On the one hand, Cal could reverse the pathologic progression of the advanced foam cells. As shown in Figure 3a suggesting that Cal not only inhibited lipid accumulation in foam cells but also effectively alleviated oxidative stress to reverse cellular foaming.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

In Vivo Fluorescent Labeling of Foam Cell-Derived Extracellular Vesicles as Circulating Biomarkers for In Vitro Detection of Atherosclerosis

Ji,

Wei,

et al. 2024

J. Am. Chem. Soc.

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Real-time monitoring of the development of atherosclerosis (AS) is key to the management of cardiovascular disease (CVD). However, existing laboratory approaches lack sensitivity and specificity, mostly due to the dearth of reliable AS biomarkers. Herein, we developed an in vivo fluorescent labeling strategy that allows specific staining of the foam cell-derived extracellular vesicles (EVs) in atherosclerotic plaques, which are released into the blood as circulating biomarkers for in vitro detection of AS. This strategy relies on a self-assembled nanoprobe that could recognize foam cells specifically, where the probe is degraded by the intracellular HClO to produce a trifluoromethylbearing boron-dipyrromethene fluorophore (termed B-CF 3 ), a lipophilic dye that can be transferred to the exosomal membranes. These circulating B-CF 3 -stained EVs can be detected directly on a fluorescence spectrometer or microplate reader without resorting to any sophisticated analytical method. This liquid-biopsy format enables early detection and real-time differentiation of lesion vulnerability during AS progression, facilitating effective CVD management.

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Role of kisspeptin-10 and betacellulin in control of feline ovarian cell functions

Loncová¹,

Fabová²,

Sirotkin³

2023

Reproductive Biology

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Involvement of obestatin, cyclin-dependent kinase and protein kinase C in control of feline ovarian cell viability and hormones release

Cited by 7 publications

References 14 publications

The growth factors amphiregulin and epiregulin are novel stimulators of feline ovarian granulosa cell functions

The growth factors amphiregulin and epiregulin are novel stimulators of feline ovarian granulosa cell functions

In Vivo Fluorescent Labeling of Foam Cell-Derived Extracellular Vesicles as Circulating Biomarkers for In Vitro Detection of Atherosclerosis

Role of kisspeptin-10 and betacellulin in control of feline ovarian cell functions

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