Involvement of opioid system in behavioral despair induced by social isolation stress in mice

Haj-Mirzaian, Arvin; Nikbakhsh, Rajan; Ramezanzadeh, Kiana; Rezaee, Mehdi; Amini-Khoei, Hossein; Haj‐Mirzaian, Arya; Ghesmati, Maria; Afshari, Khashayar; Haddadi, Nazgol‐Sadat; Dehpour, Ahmad Reza

doi:10.1016/j.biopha.2018.10.144

Cited by 39 publications

(21 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…temperature: 22 ± 2 °C, humidity: 50 ± 10%, 12-h light-dark cycle, and ad-libitum access to food and water for a period of 5 weeks. Socially conditioned mice were placed in Plexiglas boxes (25 cm × 25 cm × 15 cm) (6 mice per cage) and IC animals were placed individually in Plexiglas boxes (24 cm × 17 cm × 12 cm) [4,24]. In order to diminish handling and social interaction cages of IC animals were cleaned weekly by the same experimenter.…”

Section: Animals and Housing Conditionsmentioning

confidence: 99%

“…In hippocampus samples, after sacrificing animals in all our experimental groups, the hippocampal formation was separated and then homogenized based on our previous studies [4,37]. The homogenized samples immediately were frozen by liquid nitrogen and stored in -80 degrees C.…”

Section: Assessment Of Very Small Embryonic-like Stem Cells (Vsels) 2mentioning

confidence: 99%

“…The social isolation stress (SIS), as a chronic stressor, is a valid paradigm, which has been used as an animal model to study neurobehavioral changes [2,3]. It has been reported that social isolation could induce depressiveand anxiety-like behavior as well as decreasing seizure threshold through various mechanism including opioid system, cannabinoid system, nitrergic system, and neuro-inflammation [3][4][5][6]. Based on clinical studies and psychiatric guidelines, the diagnosis of psychiatric disorders is limited to clinical criteria [7].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The potential role of very small embryonic-like stem cells in the neuroinflammation induced by social isolation stress: Introduction of a new paradigm

Haj-Mirzaian

Khosravi

Haj‐Mirzaian

et al. 2020

Brain Research Bulletin

Self Cite

View full text Add to dashboard Cite

Background: Lack of social contacts could induce psychiatric features and lead to various behavioral and neurochemical abnormalities in rodents. Social isolation stress (SIS) is a valid paradigm of depressive-and anxiety-like behaviors in animals. It has demonstrated that psychiatric disorder could affect the peripheral blood population of very small embryonic-like stem cells (VSELs). The aim of the current study is to evaluate the role of VSELs in behavioral impairments induced by SIS through neuroinflammation in mice. Methods: Behavioral experiments were evaluated by using forced swimming test (FST), open field test (OFT), and splash test in male NMRI mice. In addition, plasma and bone marrow samples, as well as hippocampus, were collected to evaluate the population of VSELs, nitrite level, and inflammatory cytokines by using flow cytometry and ELISA. Results: Behavioral tasks showed that SIS could induce depressive-and anxiety-like behaviors in mice. Data obtained from flow cytometry showed that VSELs significantly increased in socially isolated animals in bone marrow, peripheral blood, and hippocampus. Also, TNF-α, IL-1β, and IL-6 significantly increased in hippocampal and plasma samples in socially isolated animals. Correlation analysis indicated that mice with higher VSELs counts have better results in behavioral tasks, and lower pro-inflammatory cytokines as well as nitrite level in mice. Conclusion: VSELs could be used as a biological marker to enhance diagnostic accuracy as well as predicting the prognosis. Also, increment in the VSELs counts might decrease the neuro-inflammation and subsequently improve the behavioral impairments induced by SIS.

show abstract

Section: Animals and Housing Conditionsmentioning

confidence: 99%

Section: Assessment Of Very Small Embryonic-like Stem Cells (Vsels) 2mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The potential role of very small embryonic-like stem cells in the neuroinflammation induced by social isolation stress: Introduction of a new paradigm

Haj-Mirzaian

Khosravi

Haj‐Mirzaian

et al. 2020

Brain Research Bulletin

Self Cite

View full text Add to dashboard Cite

show abstract

“…These mechanisms are thought to include dysphoria and anhedonia, which are two of the main features observed in clinical depression (Fried et al, 2016; Kendler, 2016). The κ-receptor/dynorphin system also undergoes adaptations, including changes in mRNA levels and receptor levels after repeated exposure to drugs of abuse, such as cocaine (Unterwald et al, 1994), as well as after exposure to diverse stressors (Browne et al, 2018; Haj-Mirzaian et al, 2019; Shirayama et al, 2004). Exposure to such stressors can result in depressant-like effects and anhedonia, and can also reinstate drug-taking (Beardsley et al, 2005; Haj-Mirzaian et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Profile of a short-acting κ-antagonist, LY2795050, on self-grooming behaviors, forced swim test and locomotor activity: sex comparison in mice

et al. 2021

View full text Add to dashboard Cite

Background: Novel short-acting κ(kappa)-opioid receptor selective antagonists are translational tools to examine the impact of the κ-receptor/dynorphin system in assays related to central nervous system dysfunction (e.g., substance use disorders, anhedonia and depression). The effects of such compounds have been compared in males and females under very limited conditions. Aims: The goal of this study was to examine potential sex differences in the effects of a κ-agonist and a short-acting κ-antagonist in an ethologically relevant test of anhedonia, the “splash test” of self-grooming, and also in the forced swim test and in locomotor activity. Methods: We examined the dose-dependence of grooming deficits caused by the κ-agonist U50,488 (0.1–3.2 mg/kg intraperitoneal (i.p.)) in gonadally intact adult male and female C57BL/6J mice. We then compared the effects of the short-acting κ-antagonist LY2795050 ((3-chloro-4-(4-(((2S)-2-pyridin-3-ylpyrrolidin-1-yl)methyl) phenoxy)benzamide)); 0.032–0.1 mg/kg i.p.) in blocking grooming deficits caused by U50,488 (3.2 mg/kg). The effects of LY2795050 were also studied in the forced swim test (FST). The effects of LY2795050 in blocking the locomotor depressant effects of U50,488 (10 mg/kg) were also studied. Results: U50,488 produced dose-dependent grooming deficits in male and female mice, and LY2795050 prevented these effects. In contrast, LY2795050 decreased immobility in the FST in males at a dose of 0.1 mg/kg, but not in females, up to a dose of 0.32 mg/kg. Also, LY2795050 (0.32 mg/kg) prevented and also reversed the locomotor-depressant effects of U50,488 (10 mg/kg), in males and females. Conclusions: This study further implicates the κ-receptor system in ethologically relevant aspects of anhedonia, and confirms sexual dimorphism in some behavioral effects of novel κ-antagonists.

show abstract

“…The same effect was not observed in non-predisposed Wistar rats, suggesting that predisposition to the effects of stress is a determining factor for behavior despair following SIS ( Zanier-Gomes et al, 2015 ). Moreover, several reports also revealed that SIS significantly increased the immobility time of NMRI and C57BL/6J mice or Sprague-Dawley rats submitted to the FST ( Chan et al, 2017 ; Cho et al, 2017 ; Dávila-Hernández et al, 2018 ; Haj-Mirzaian et al, 2015 , Haj-Mirzaian et al, 2019 ). The discrepancies of our results in the behavior despair test, compared to the literature data, could be due to several factors, including biological factors such as strain, age, body weight, gender, and individual differences between animals.…”

Section: Discussionmentioning

confidence: 93%

The effect of voluntary wheel running on the antioxidant status is dependent on sociability conditions

Cunha

Machado

Mancini

et al. 2020

Pharmacology Biochemistry and Behavior

View full text Add to dashboard Cite

Voluntary wheel running is widely used as a physical activity (PA) model in rodents, but most studies investigate the beneficial effects of this intervention in socially isolated mice. Social isolation stress (SIS) is associated with vulnerability to oxidative stress and reduced mitochondrial activity. Thus, the aim of this study was to investigate the effects of free access to a running wheel for 21 days on the various markers of the cellular redox/antioxidant status as well as mitochondrial function of mice subjected to SIS or maintained in groups of 3 in the homecage. SIS increased thiobarbituric acid reactive substance (TBARS) levels in the cerebral cortex, and PA intervention was not able to reverse such alteration. PA reduced TBARS levels in the liver of grouped mice and gastrocnemius of socially isolated mice. PA increased nonprotein thiol (NPSH) levels in the cerebral cortex of grouped mice. Furthermore, socially isolated mice presented lower glutathione peroxidase (GPx) activity in the cerebellum and gastrocnemius, and glutathione reductase (GR) activity in the cerebral cortex and liver. By contrast, SIS induced higher GPx activity in the cerebral cortex and heart. PA reduced GPx (cerebral cortex) and GR (cerebral cortex and liver) activities of socially isolated mice. SIS caused higher activity of mitochondrial complexes I and II in the cerebral cortex, and the PA paradigm was not able to alter this effect. Interestingly, the PA produced antidepressant-like effect at both SIS and control groups. In conclusion, the results showed the influence of SIS for the effects of PA on the antioxidant status, but not on the mitochondrial function and emotionality.

show abstract

Involvement of opioid system in behavioral despair induced by social isolation stress in mice

Cited by 39 publications

References 66 publications

The potential role of very small embryonic-like stem cells in the neuroinflammation induced by social isolation stress: Introduction of a new paradigm

The potential role of very small embryonic-like stem cells in the neuroinflammation induced by social isolation stress: Introduction of a new paradigm

Profile of a short-acting κ-antagonist, LY2795050, on self-grooming behaviors, forced swim test and locomotor activity: sex comparison in mice

The effect of voluntary wheel running on the antioxidant status is dependent on sociability conditions

Contact Info

Product

Resources

About