Involvement of oxidative stress in bactericidal activity of 2-(2-nitrovinyl) furan against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus

Involvement of oxidative stress in protocatechuic acid‐mediated bacterial lethality

“…Imbalance in the antioxidant defense system and ROS generation leading to oxidative damage of cellular macromolecules such as proteins, lipids, and DNA have been implicated in antibiotics and antimicrobials mediated bacterial lethality (Ajiboye et al., ,b; Dwyer et al., ; Lobritz et al., ; Samoilova, Smirnova, Muzyka, & Oktyabrsky, ; Wang, Zhao, Malik, & Drlica, ; Zhao & Drlica, ; Zhao, Hong, & Drlica, ). Generation of ROS (superoxide anion radical) have been demonstrated in the prooxidant activity of protocatechuic acid (Zeraik et al., ).…”

“…Susceptibility of E. coli , P. aeruginosa , and S. aureus to protocatechuic acid was investigated using the procedure described by Ajiboye et al. (). Briefly, organisms were grown overnight in LB medium, harvested by centrifugation, and resuspended in 50 ml fresh medium (LB) to OD 600 = 0.1, and grown aerobically at 37°C in 250 ml flask to OD 600 = 0.2.…”

“…The superoxide anion of the cells was calculated using the molar extinction coefficient of 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide formazan (17,000 M −1 cm −1 at pH 7.4–8.0) as described by Ajiboye et al. ().…”

“…Recent studies have documented the involvement of ROS in bacterial lethality of antibiotics and antimicrobials (Ajiboye et al., ,b,c,d; Kohanski, Dwyer, Hayete, Lawrence, & Collins, ). In these studies, hydroxyl radical, generated through the catalytic action of ferrous ion on hydrogen peroxide (Fenton chemistry) (Lemire, Harrison, & Turner, ), is responsible for bacterial death.…”

Lophirones B and C prevent aflatoxin B1-induced oxidative stress and DNA fragmentation in rat hepatocytes

“…The concentration of intracellular superoxide radical anion in rat hepatocytes was measured using the procedure described by Ajiboye et al (2016). Briefly, hepatocytes (0.5 mL) were incubated with 2.5 mL nitroblue tetrazolium (1 mg/mL) for 30 min at 37 C. Then 0.5 mL of 0.1 M HCl was added.…”