Involvement of plasmids in lactose metabolism in Lactobacillus casei suggested by genetic experiments

L'état des connaissances en matière de nutrition des bactéries lactiques

“…Trois plasmides sont associés à des étapes semblables du métabolisme du lactose, bien qu'aucune région d'homologie évidente ne puisse être détectée. En dehors des streptocoques du groupe N, des gènes du métabolisme du lactose ont été associés à des plasmides chez L. helveticus subsp jugurti (Smiley et Fryder, 1978), L. casei (Hofer, 1977), L. casei subsp casei (Chassy et al, 1978).…”

Looking backward and forward at the practical applications of genetic researches on lactic acid bacteria

“…Chassy et al [141] were the first to note the presence of plasmids in this genus and publications have since confirmed this [142,143]. A general method for plasmid isolation from lactobacilli has been published [144] and the plasmids have been shown to encode for lactose fermentation [145][146][147], drug resistance [148][149][150][151] and to be involved in lactic acid production and glucosamine fermentation [152]. Lee-Wickner and Chassy [153] communicated a procedure to produce and regenerate Lactobacillus protoplasts, a prerequisite to protoplast transformation and fusion.…”

Characterization and molecular cloning of cryptic plasmids isolated from Lactobacillus casei

“…Suitable vectors might be found among those already used successfully with other gram-positive general; alternatively, vectors based on Lactobacillus indigenous plasmids could be constructed. Plasmids are widely distributed among members of the genus Lactobacillus (4,5,12,14,17,18,24,29,33). Lactose metabolism in L. casei (5,12) has been shown to be dependent on the presence of plasmids.…”

“…Plasmids are widely distributed among members of the genus Lactobacillus (4,5,12,14,17,18,24,29,33). Lactose metabolism in L. casei (5,12) has been shown to be dependent on the presence of plasmids. The phospho-pgalactoside galactohydrolase gene, one of the lactose metabolic genes, has been cloned from 35-kilobase pair (kbp) lactose plasmid pLZ64 into Escherichia coli K-12 (20).…”