Rice, as a staple food for a large proportion of the world, is a vitally important food crop. More than 3.5 billion people in the world depend on rice, which provides 20% of human calorific needs per day. This paper aims to analyze several sociodemographic factors that affect stakeholders’ attitudes toward genetically modified (GM) rice in Malaysia. This research involved a total of 509 adult respondents in the Klang Valley. The results of the study showed that Malaysian stakeholders’ attitudes toward GM rice can be classified as positive. They perceived GM rice as having moderate benefits and risks, and they considered the moral aspects to be moderate, as was religious acceptance. One-way MANOVA initially detected a significant difference in attitude toward GM rice across stakeholder groups, educational level, and age. Additionally, univariate as well as post hoc analysis, confirmed significant differences in attitudes across stakeholders (perceived moral concerns, religious acceptance, and encouragement); educational level (perceived benefits, religious acceptance); and age (religious acceptance). These findings are useful for scientists and government regulators in terms of understanding the effects of sociodemographic factors on attitudes toward GM rice in Malaysia.