Involvement of polyamines in the root nodule regulation of soybeans (Glycine max)

Terakado-Tonooka, Junko; Fujihara, Shinsuke

doi:10.3117/plantroot.2.46

Cited by 14 publications

(6 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…So it might be reasonable that plant hormones regulate nodule development [40]. For example, salicylic acid [41], abscisic acid [42], methyl jasmonic acid [43] and polyamine [44] are proposed to be involved in autoregulation of nodulation in leguminous plants. The autoregulation of nodulation might be regulated through interplay of several signaling compounds.…”

Section: Resultsmentioning

confidence: 99%

Autoregulation of Nodulation in Soybean Plants

Tanabata¹,

Ohyama²

2014

Advances in Biology and Ecology of Nitrogen Fixation

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Autoregulation of Nodulation in Soybean Plants

Tanabata¹,

Ohyama²

2014

Advances in Biology and Ecology of Nitrogen Fixation

View full text Add to dashboard Cite

“…1b , bottom). Whereas the asymmetric distribution of SAM throughout the infection zone sheds a different light in its role in downstream BNF processes, given that SAM occupies a central role in both polyamine [ 8 ] and phosphatidylcholine (PCs) biosynthesis [ 9 ], molecules involved in nodule growth [ 8 ] and rhizobia-plant recognition [ 9 ], respectively. Further, ADP plays a central role during BNF, and its abundance is a key indicator of the energetic and oxidative state within the nodule [ 10 ].…”

mentioning

confidence: 99%

Observed metabolic asymmetry within soybean root nodules reflects unexpected complexity in rhizobacteria-legume metabolite exchange

et al. 2018

View full text Add to dashboard Cite

In this study, the three-dimensional spatial distributions of a number of metabolites involved in regulating symbiosis and biological nitrogen fixation (BNF) within soybean root nodules were revealed using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). While many metabolites exhibited distinct spatial compartmentalization, some metabolites were asymmetrically distributed throughout the nodule (e.g., S-adenosylmethionine). These results establish a more complex metabolic view of plant–bacteria symbiosis (and BNF) within soybean nodules than previously hypothesized. Collectively these findings suggest that spatial perspectives in metabolic regulation should be considered to unravel the overall complexity of interacting organisms, like those relating to associations of nitrogen-fixing bacteria with host plants.

show abstract

“…2-S4c), as expected due to its role in nodule organogenesis. Also, SAM is a polyamine that is known to mediate hormone-induced plant responses in nodulation (Minder et al, 2001;Terakado-Tonooka & Fujihara, 2008). This observation was also made in our previous MALDI-MSI study , where SAM was highly present in the WT nodule compared to the nifH nodule, but showed an asymmetric distribution in the infection zone of the nodule.…”

Section: Carbon and Nitrogen Metabolism Detected In Nitrogen-fixing Soybean Rootssupporting

confidence: 68%

Exploring the symbiotic relationship of plants and microbes by mass spectrometry imaging

Agtuca¹

View full text Add to dashboard Cite

Current practices in agriculture make use of chemical inputs including nitrogen fertilizers to provide high crop yields since nitrogen is often the limiting nutrient in field soil. However, nitrogen fertilizer use has negative impacts; such as due to extensive chemical runoff into waterways, increases in use of fossil fuel-derived energy with concomitant increases in greenhouse gas emissions, and accumulating costs correlated with fertilizer distribution. In contrast, biological nitrogen fixation (BNF), offers an alternative way to provide crops with required nitrogen. BNF is a process by which specific microbes convert nitrogen gas into ammonia, a form readily utilized by the plant. This dissertation focuses on two, symbiotic BNF systems: one, the intimate association between Bradyrhizobium japonicum and soybean and, two, the less intimate, associative BNF interaction between the model grass species Setaria viridis and the endophytic, plant growth promoting bacterium (PGPB) Herbaspirillum seropedicae. While these systems have both been studied at the level of the transcriptome and, to some degree, the proteome, much less is known as to how the metabolome (metabolites) change in response to bacterial colonization and nitrogen fixation. In both of these symbiotic associations, bacterial colonization is limited to specific sites and, therefore, relatively few plant cells are directly exposed to the bacteria. Therefore, in order to limit any dilution of signal due to non-responding tissues, we employed a mass spectroscopy imaging method, which allowed us to specifically target plant cells nearby or in direct contact with the bacteria. We applied an advanced and highthroughput technique called laser ablation electrospray ionization mass spectrometry (LAESI-MS) for in situ metabolic profiling. This reliable approach requires minimal sample preparation, while preserving the spatial information of biomolecules in their native state. The results provide information regarding those metabolic pathways that play a significant role in the symbiosis. Overall, the data demonstrate that LAESI-MS holds tremendous potential for use in further studies of plant-microbe interactions, as well as other plant processes.

show abstract

Involvement of polyamines in the root nodule regulation of soybeans (Glycine max)

Cited by 14 publications

References 57 publications

Autoregulation of Nodulation in Soybean Plants

Autoregulation of Nodulation in Soybean Plants

Observed metabolic asymmetry within soybean root nodules reflects unexpected complexity in rhizobacteria-legume metabolite exchange

Exploring the symbiotic relationship of plants and microbes by mass spectrometry imaging

Contact Info

Product

Resources

About